Curated Optogenetic Publication Database

Search precisely and efficiently by using the advantage of the hand-assigned publication tags that allow you to search for papers involving a specific trait, e.g. a particular optogenetic switch or a host organism.

Showing 1 - 2 of 2 results

Transient light-activated gene expression in Chinese hamster ovary cells.

blue CRY2/CIB1 CHO-DG44 CHO-K1 Transgene expression
BMC Biotechnol, 4 Feb 2021 DOI: 10.1186/s12896-021-00670-1 Link to full text
Abstract: Chinese hamster ovary (CHO) cells are widely used for industrial production of biopharmaceuticals. Many genetic, chemical, and environmental approaches have been developed to modulate cellular pathways to improve titers. However, these methods are often irreversible or have off-target effects. Development of techniques which are precise, tunable, and reversible will facilitate temporal regulation of target pathways to maximize titers. In this study, we investigate the use of optogenetics in CHO cells. The light-activated CRISPR-dCas9 effector (LACE) system was first transiently transfected to express eGFP in a light-inducible manner. Then, a stable system was tested using lentiviral transduction.

Light induced expression of β-glucosidase in Escherichia coli with autolysis of cell.

blue YtvA E. coli Transgene expression
BMC Biotechnol, 7 Nov 2017 DOI: 10.1186/s12896-017-0402-1 Link to full text
Abstract: β-Glucosidase has attracted substantial attention in the scientific community because of its pivotal role in cellulose degradation, glycoside transformation and many other industrial processes. However, the tedious and costly expression and purification procedures have severely thwarted the industrial applications of β-glucosidase. Thus development of new strategies to express β-glucosidases with cost-effective and simple procedure to meet the increasing demands on enzymes for biocatalysis is of paramount importance.
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