Materials

A collection of useful material sources for optogenetic experiments.

Plasmids

AddGene - The optogenetics collection

Illumination devices aka optogenetics light boxes

“LAVA device” — a DIY tutorial — A system for experiments in 24- and 96-well plates, which allows user-defined illumination patterns. It can be optimized either for high illumination intensities or for high illumination uniformity.

+ A detailed step-by-step tutorial for the above system (v. 2020)

“optoPlate-96” — a comprehensive step-by-step DIY tutorial (v. 2019) — A system for high-throughput three-color optogenetics experiments that allows simultaneous manipulation of common red- and blue-light-sensitive optogenetic probes. It enables illumination of individual wells in 96-well plates without cross-illumination between wells, and includes an active cooling system to minimize sample heating during light-intensive experiments. Suitable also for experiments in 384-well plates.

+ "optoConfig-96" — A Python-based graphical user interface available for Windows and MacOS for configuration of the optoPlate-96.

“Light Plate Apparatus” — a comprehensive DIY tutorial (v. 2016) — A system for experiments in a 24-well format, allows for simultaneous illumination with two independently programmable custom (310 to 1550 nm) colors of illumination.

+ A detailed step-by-step tutorial for the improved above system (v. 2019)

“Optogenetics Light Box” — a DIY tutorial and commissioning (v. 2018) — A system for experiments in a 12-well format, allows for one custom color illumination. To build one of these light boxes, ask the author (J. Vrana) for further step-by-step details. The materials list, Arduino code and the laser cutter files are already available on the page.

"opto biolabs" — a spin-off from the University of Freiburg, Germany — opto biolabs develops illumination devices and is your development partner for tailor-made solutions for optogenetics research expanding the experimental spectrum and increasing efficiency in research.

Learning

An Introduction to Optogenetics — a mini (~40 min) online course from EMBL
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