Curated Optogenetic Publication Database

101.

Strategies for the photo-control of endogenous protein activity.

blue Cryptochromes Fluorescent proteins LOV domains Review

Curr Opin Struct Biol, 28 Nov 2016 DOI: 10.1016/j.sbi.2016.11.014 Link to full text

Abstract: Photo-controlled or 'optogenetic' effectors interfacing with endogenous protein machinery allow the roles of endogenous proteins to be probed. There are two main approaches being used to develop optogenetic effectors: (i) caging strategies using photo-controlled conformational changes, and (ii) protein relocalization strategies using photo-controlled protein-protein interactions. Numerous specific examples of these approaches have been reported and efforts to develop general methods for photo-control of endogenous proteins are a current focus. The development of improved screening and selection methods for photo-switchable proteins would advance the field.

102.

LOVTRAP: an optogenetic system for photoinduced protein dissociation.

blue LOVTRAP HEK293 HeLa in vitro Control of cytoskeleton / cell motility / cell shape

Nat Methods, 18 Jul 2016 DOI: 10.1038/nmeth.3926 Link to full text

Abstract: LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.

103.

A comparison of the substrate specificities of endo-beta-N-acetylglucosaminidases from Streptomyces griseus and Diplococcus Pneumoniae.

blue BLUF domains Cryptochromes LOV domains CHO DP-12 Background

Biochem Biophys Res Commun, 3 Nov 1975 DOI: 10.1007/s00253-024-13363-4 Link to full text

Abstract: Abstract not available.