Curated Optogenetic Publication Database

Search precisely and efficiently by using the advantage of the hand-assigned publication tags that allow you to search for papers involving a specific trait, e.g. a particular optogenetic switch or a host organism.

Showing 1 - 2 of 2 results

LOVTRAP: an optogenetic system for photoinduced protein dissociation.

blue LOVTRAP HEK293 HeLa in vitro Control of cytoskeleton / cell motility / cell shape
Nat Methods, 18 Jul 2016 DOI: 10.1038/nmeth.3926 Link to full text
Abstract: LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.

Manipulation of endogenous kinase activity in living cells using photoswitchable inhibitory peptides.

blue AsLOV2 Cos-7 HEK293 primary mouse cortical neurons Signaling cascade control Control of cytoskeleton / cell motility / cell shape
ACS Synth Biol, 13 Jun 2014 DOI: 10.1021/sb5001356 Link to full text
Abstract: Optogenetic control of endogenous signaling can be an important tool for probing cell behavior. Using the photoresponse of the LOV2 domain of Avena sativa phototropin 1, we developed analogues of kinase inhibitors whose activity is light dependent. Inhibitory peptides were appended to the Jα helix, where they potently inhibited kinases in the light but were sterically blocked from kinase interaction in the dark. Photoactivatable inhibitors for cyclic-AMP dependent kinase (PKA) and myosin light chain kinase (MLCK) are described, together with studies that shed light on proper positioning of the peptides in the LOV domain. These inhibitors altered endogenous signaling in living cells and produced light-dependent changes in cell morphodynamics.
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