bPAC (BlaC)

Photoreceptor bPAC (BlaC)
Binding partner /
Cofactor FAD or FMN
Source organism Beggiatoa sp.
Mode of action cAMP production
Excitation wavelength 450 nm
Reversion wavelength dark
Excitation time seconds
Reversion time tens of seconds
Crystal structure of the bPAC homodimer, ground state. (PDB ID: 5MBE)

bPAC (BlaC)-derived switches:

Related original publications

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  1. Optogenetic manipulation of neuronal and cardiomyocyte functions in zebrafish using microbial rhodopsins and adenylyl cyclases. Elife, 2023
  2. All-optical mapping of cAMP transport reveals rules of sub-cellular localization. bioRxiv, 2023
  3. Optogenetic Stimulation Array for Confocal Microscopy Fast Transient Monitoring. TBioCAS, 2022
  4. Photoactivated adenylyl cyclases attenuate sepsis-induced cardiomyopathy by suppressing macrophage-mediated inflammation. Front Immunol, 2022
  5. GPCR-dependent spatiotemporal cAMP generation confers functional specificity in cardiomyocytes and cardiac responses. bioRxiv, 2022
  6. A cAMP signalosome in primary cilia drives gene expression and kidney cyst formation. EMBO Rep, 2022
  7. Soluble cyclase-mediated nuclear cAMP synthesis is sufficient for cell proliferation. bioRxiv, 2022
  8. Morphogen Directed Coordination of GPCR Activity Promotes Primary Cilium Function for Downstream Signaling. bioRxiv, 2022
  9. Hypothalamic dopamine neurons motivate mating through persistent cAMP signalling. Nature, 2021
  10. Endosomal cAMP production broadly impacts the cellular phosphoproteome. J Biol Chem, 2021
  11. mem-iLID, a fast and economic protein purification method. Biosci Rep, 2021
  12. Endosomal cAMP production broadly impacts the cellular phosphoproteome. bioRxiv, 2021
  13. Vertebrate cells differentially interpret ciliary and extraciliary cAMP. Cell, 2021
  14. Optogenetic tools for manipulation of cyclic nucleotides, functionally coupled to CNG-channels. Br J Pharmacol, 2021
  15. Optogenetic modulation of real-time nanoscale dynamics of HCN channels using photoactivated adenylyl cyclases. RSC Chem Biol, 2021
  16. Light-Induced Change of Arginine Conformation Modulates the Rate of Adenosine Triphosphate to Cyclic Adenosine Monophosphate Conversion in the Optogenetic System Containing Photoactivated Adenylyl Cyclase. J Chem Inf Model, 2021
  17. Optogenetic Modification of Pseudomonas aeruginosa Enables Controllable Twitching Motility and Host Infection. ACS Synth Biol, 2021
  18. Biphasic Response of Protein Kinase A to Cyclic Adenosine Monophosphate Triggers Distinct Epithelial Phenotypes. bioRxiv, 2020
  19. SynaptoPAC, an optogenetic tool for induction of presynaptic plasticity. J Neurochem, 2020
  20. Dual Activation of cAMP Production Through Photostimulation or Chemical Stimulation. Methods Mol Biol, 2020
  21. Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium. Elife, 2020
  22. Optogenetic Manipulation of Postsynaptic cAMP Using a Novel Transgenic Mouse Line Enables Synaptic Plasticity and Enhances Depolarization Following Tetanic Stimulation in the Hippocampal Dentate Gyrus. Front Neural Circuits, 2020
  23. Booster, a Red-Shifted Genetically Encoded Förster Resonance Energy Transfer (FRET) Biosensor Compatible with Cyan Fluorescent Protein/Yellow Fluorescent Protein-Based FRET Biosensors and Blue Light-Responsive Optogenetic Tools. ACS Sens, 2020
  24. An optogenetic tool for induced protein stabilization based on the Phaeodactylum tricornutum aureochrome 1a LOV domain. J Mol Biol, 2020
  25. Primary Cilia Signaling Promotes Axonal Tract Development and Is Disrupted in Joubert Syndrome-Related Disorders Models. Dev Cell, 2019
  26. Amelioration of Diabetes in a Murine Model upon Transplantation of Pancreatic β-Cells with Optogenetic Control of Cyclic Adenosine Monophosphate. ACS Synth Biol, 2019
  27. Compartmentalized cAMP Generation by Engineered Photoactivated Adenylyl Cyclases. Cell Chem Biol, 2019
  28. Using a Robust and Sensitive GFP-Based cGMP Sensor for Real Time Imaging in Intact Caenorhabditis elegans. Genetics, 2019
  29. Cyclic Nucleotide-Specific Optogenetics Highlights Compartmentalization of the Sperm Flagellum into cAMP Microdomains. Cells, 2019
  30. Luminescence-activated nucleotide cyclase regulates spatial and temporal cAMP synthesis. J Biol Chem, 2018
  31. Potassium channel-based optogenetic silencing. Nat Commun, 2018
  32. Synthetic Light-Activated Ion Channels for Optogenetic Activation and Inhibition. Front Neurosci, 2018
  33. Modulation of cyclic nucleotide-mediated cellular signaling and gene expression using photoactivated adenylyl cyclase as an optogenetic tool. Sci Rep, 2017
  34. Optogenetic regulation of insulin secretion in pancreatic β-cells. Sci Rep, 2017
  35. Red fluorescent protein-based cAMP indicator applicable to optogenetics and in vivo imaging. Sci Rep, 2017
  36. Fast cAMP Modulation of Neurotransmission via Neuropeptide Signals and Vesicle Loading. Curr Biol, 2017
  37. Model-guided optogenetic study of PKA signaling in budding yeast. Mol Biol Cell, 2016
  38. Structural insight into photoactivation of an adenylate cyclase from a photosynthetic cyanobacterium. Proc Natl Acad Sci USA, 2016
  39. The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling. Sci Signal, 2015
  40. Manipulation of Interrenal Cell Function in Developing Zebrafish Using Genetically Targeted Ablation and an Optogenetic Tool. Endocrinology, 2015
  41. Controlling fertilization and cAMP signaling in sperm by optogenetics. Elife, 2015
  42. Optogenetic elevation of endogenous glucocorticoid level in larval zebrafish. Front Neural Circuits, 2013
  43. Natural and engineered photoactivated nucleotidyl cyclases for optogenetic applications. J Biol Chem, 2010
  44. Light modulation of cellular cAMP by a small bacterial photoactivated adenylyl cyclase, bPAC, of the soil bacterium Beggiatoa. J Biol Chem, 2010