bPAC (BlaC)

Photoreceptor bPAC (BlaC)
Binding partner /
Cofactor FAD or FMN
Source organism Beggiatoa sp.
Mode of action cAMP production
Excitation wavelength 450 nm
Reversion wavelength dark
Excitation time seconds
Reversion time tens of seconds
Crystal structure of the bPAC homodimer, ground state. (PDB ID: 5MBE)

bPAC (BlaC)-derived switches:

Related original publications

View in Publication Search
  1. The caloric value of food intake structurally adjusts a neuronal mushroom body circuit mediating olfactory learning in Drosophila. Learn Mem, 2024
  2. Optogenetic induction of chronic glucocorticoid exposure in early-life leads to blunted stress-response in larval zebrafish. Eur J Neurosci, 2024
  3. Live-cell fluorescence imaging and optogenetic control of PKA kinase activity in fission yeast Schizosaccharomyces pombe. Yeast, 2024
  4. Light-Mediated Enhancement of Glucose-Stimulated Insulin Release of Optogenetically Engineered Human Pancreatic Beta-Cells. ACS Synth Biol, 2024
  5. Mapping the Cellular Distribution of an Optogenetic Protein Using a Light-Stimulation Grid Mapping the Cellular Distribution of an Optogenetic Protein Using a Light-Stimulation Grid. J Vis Exp, 2024
  6. Live-cell fluorescence imaging and optogenetic control of PKA kinase activity in fission yeast Schizosaccharomyces pombe. bioRxiv, 2024
  7. Neuropeptidergic regulation of neuromuscular signaling in larval zebrafish alters swimming behavior and synaptic transmission. bioRxiv, 2024
  8. Lifelong molecular consequences of high Glucocorticoids exposure during development bioRxiv, 2024
  9. Pathogen infection induces sickness behaviors by recruiting neuromodulatory systems linked to stress and satiety in C. elegans. bioRxiv, 2024
  10. Optogenetic demonstration of the involvement of SMA-negative mural cells in the regulation of cerebral blood flow. Front Physiol, 2023
  11. Rab3 mediates cyclic AMP-dependent presynaptic plasticity and olfactory learning. bioRxiv, 2023
  12. An Integrated Optogenetic and Bioelectronic Platform for Regulating Cardiomyocyte Function. bioRxiv, 2023
  13. Optogenetic manipulation of neuronal and cardiomyocyte functions in zebrafish using microbial rhodopsins and adenylyl cyclases. Elife, 2023
  14. All-optical mapping of cAMP transport reveals rules of sub-cellular localization. bioRxiv, 2023
  15. Soluble cyclase-mediated nuclear cAMP synthesis is sufficient for cell proliferation. Proc Natl Acad Sci U S A, 2023
  16. Optogenetic Stimulation Array for Confocal Microscopy Fast Transient Monitoring. TBioCAS, 2022
  17. Photoactivated adenylyl cyclases attenuate sepsis-induced cardiomyopathy by suppressing macrophage-mediated inflammation. Front Immunol, 2022
  18. GPCR-dependent spatiotemporal cAMP generation confers functional specificity in cardiomyocytes and cardiac responses. bioRxiv, 2022
  19. A cAMP signalosome in primary cilia drives gene expression and kidney cyst formation. EMBO Rep, 2022
  20. Morphogen Directed Coordination of GPCR Activity Promotes Primary Cilium Function for Downstream Signaling. bioRxiv, 2022
  21. Hypothalamic dopamine neurons motivate mating through persistent cAMP signalling. Nature, 2021
  22. Endosomal cAMP production broadly impacts the cellular phosphoproteome. J Biol Chem, 2021
  23. mem-iLID, a fast and economic protein purification method. Biosci Rep, 2021
  24. Vertebrate cells differentially interpret ciliary and extraciliary cAMP. Cell, 2021
  25. Optogenetic tools for manipulation of cyclic nucleotides, functionally coupled to CNG-channels. Br J Pharmacol, 2021
  26. Optogenetic modulation of real-time nanoscale dynamics of HCN channels using photoactivated adenylyl cyclases. RSC Chem Biol, 2021
  27. Light-Induced Change of Arginine Conformation Modulates the Rate of Adenosine Triphosphate to Cyclic Adenosine Monophosphate Conversion in the Optogenetic System Containing Photoactivated Adenylyl Cyclase. J Chem Inf Model, 2021
  28. Optogenetic Modification of Pseudomonas aeruginosa Enables Controllable Twitching Motility and Host Infection. ACS Synth Biol, 2021
  29. Biphasic Response of Protein Kinase A to Cyclic Adenosine Monophosphate Triggers Distinct Epithelial Phenotypes. bioRxiv, 2020
  30. SynaptoPAC, an optogenetic tool for induction of presynaptic plasticity. J Neurochem, 2020
  31. Dual Activation of cAMP Production Through Photostimulation or Chemical Stimulation. Methods Mol Biol, 2020
  32. Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium. Elife, 2020
  33. Optogenetic Manipulation of Postsynaptic cAMP Using a Novel Transgenic Mouse Line Enables Synaptic Plasticity and Enhances Depolarization Following Tetanic Stimulation in the Hippocampal Dentate Gyrus. Front Neural Circuits, 2020
  34. Booster, a Red-Shifted Genetically Encoded Förster Resonance Energy Transfer (FRET) Biosensor Compatible with Cyan Fluorescent Protein/Yellow Fluorescent Protein-Based FRET Biosensors and Blue Light-Responsive Optogenetic Tools. ACS Sens, 2020
  35. An optogenetic tool for induced protein stabilization based on the Phaeodactylum tricornutum aureochrome 1a LOV domain. J Mol Biol, 2020
  36. Primary Cilia Signaling Promotes Axonal Tract Development and Is Disrupted in Joubert Syndrome-Related Disorders Models. Dev Cell, 2019
  37. Amelioration of Diabetes in a Murine Model upon Transplantation of Pancreatic β-Cells with Optogenetic Control of Cyclic Adenosine Monophosphate. ACS Synth Biol, 2019
  38. Compartmentalized cAMP Generation by Engineered Photoactivated Adenylyl Cyclases. Cell Chem Biol, 2019
  39. Using a Robust and Sensitive GFP-Based cGMP Sensor for Real Time Imaging in Intact Caenorhabditis elegans. Genetics, 2019
  40. Cyclic Nucleotide-Specific Optogenetics Highlights Compartmentalization of the Sperm Flagellum into cAMP Microdomains. Cells, 2019
  41. Luminescence-activated nucleotide cyclase regulates spatial and temporal cAMP synthesis. J Biol Chem, 2018
  42. Potassium channel-based optogenetic silencing. Nat Commun, 2018
  43. Synthetic Light-Activated Ion Channels for Optogenetic Activation and Inhibition. Front Neurosci, 2018
  44. Modulation of cyclic nucleotide-mediated cellular signaling and gene expression using photoactivated adenylyl cyclase as an optogenetic tool. Sci Rep, 2017
  45. Optogenetic regulation of insulin secretion in pancreatic β-cells. Sci Rep, 2017
  46. Red fluorescent protein-based cAMP indicator applicable to optogenetics and in vivo imaging. Sci Rep, 2017
  47. Fast cAMP Modulation of Neurotransmission via Neuropeptide Signals and Vesicle Loading. Curr Biol, 2017
  48. Model-guided optogenetic study of PKA signaling in budding yeast. Mol Biol Cell, 2016
  49. Structural insight into photoactivation of an adenylate cyclase from a photosynthetic cyanobacterium. Proc Natl Acad Sci USA, 2016
  50. The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling. Sci Signal, 2015
  51. Manipulation of Interrenal Cell Function in Developing Zebrafish Using Genetically Targeted Ablation and an Optogenetic Tool. Endocrinology, 2015
  52. Controlling fertilization and cAMP signaling in sperm by optogenetics. Elife, 2015
  53. Serotonin and the neuropeptide PDF initiate and extend opposing behavioral states in C. elegans. Cell, 2013
  54. Optogenetic elevation of endogenous glucocorticoid level in larval zebrafish. Front Neural Circuits, 2013
  55. Natural and engineered photoactivated nucleotidyl cyclases for optogenetic applications. J Biol Chem, 2010
  56. Light modulation of cellular cAMP by a small bacterial photoactivated adenylyl cyclase, bPAC, of the soil bacterium Beggiatoa. J Biol Chem, 2010