A single-chain and fast-responding light-inducible Cre recombinase as a novel optogenetic switch. Elife, 2021
Optical regulation of endogenous RhoA reveals switching of cellular responses by signal amplitude. bioRxiv, 2021
Transient light-activated gene expression in Chinese hamster ovary cells. BMC Biotechnol, 2021
A CRISPR-Cas9-Based Near-Infrared Upconversion-Activated DNA Methylation Editing System. ACS Appl Mater Interfaces, 2021
Transcription activation is enhanced by multivalent interactions independent of liquid-liquid phase separation. bioRxiv, 2021
A synthetic BRET-based optogenetic device for pulsatile transgene expression enabling glucose homeostasis in mice. Nat Commun, 2021
Control of SRC molecular dynamics encodes distinct cytoskeletal responses by specifying signaling pathway usage. J Cell Sci, 2021
Optogenetic Control of Phosphatidylinositol (3,4,5)‐triphosphate Production by Light‐sensitive Cryptochrome Proteins on the Plasma Membrane. Chin J Chem, 2021
Random sub-diffusion and capture of genes by the nuclear pore reduces dynamics and coordinates interchromosomal movement. bioRxiv, 2021
Optogenetic control of small GTPases reveals RhoA mediates intracellular calcium signaling. J Biol Chem, 2021
Synthetic gene networks recapitulate dynamic signal decoding and differential gene expression. bioRxiv, 2021
A Light-Inducible Split-dCas9 System for Inhibiting the Progression of Bladder Cancer Cells by Activating p53 and E-cadherin. Front Mol Biosci, 2021
Liquid-liquid phase separation of light-inducible transcription factors increases transcription activation in mammalian cells and mice. Sci Adv, 2021
Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse. Proc Natl Acad Sci U S A, 2020
Regulating enzymatic reactions in Escherichia coli utilizing light-responsive cellular compartments based on liquid-liquid phase separation. bioRxiv, 2020
Spatio-temporal Control of ERK Pulse Frequency Coordinates Fate Decisions during Mammary Acinar Morphogenesis. bioRxiv, 2020
Single-Protein Tracking to Study Protein Interactions During Integrin-Based Migration. Methods Mol Biol, 2020
Light-inducible Deformation of Mitochondria in Live Cells. bioRxiv, 2020
Mechanical competition alters the cellular interpretation of an endogenous genetic programme. bioRxiv, 2020
Multichromatic Control of Signaling Pathways in Mammalian Cells. Adv Biosyst, 2020
Optical control of ERK and AKT signaling promotes axon regeneration and functional recovery of PNS and CNS in Drosophila. Elife, 2020
Control of Cell Migration Using Optogenetics. Methods Mol Biol, 2020
Activation of Cdc42 GTPase upon CRY2-Induced Cortical Recruitment Is Antagonized by GAPs in Fission Yeast. Cells, 2020
CL6mN: Rationally Designed Optogenetic Photoswitches with Tunable Dissociation Dynamics. ACS Synth Biol, 2020
A non-invasive far-red light-induced split-Cre recombinase system for controllable genome engineering in mice. Nat Commun, 2020
Photo-SNAP-tag, a Light-Regulated Chemical Labeling System. ACS Chem Biol, 2020
Optogenetic Control of Gene Expression Using Cryptochrome 2 and a Light-Activated Degron. Methods Mol Biol, 2020
βH-spectrin is required for ratcheting apical pulsatile constrictions during tissue invagination. EMBO Rep, 2020
Early But Not Delayed Optogenetic RAF Activation Promotes Astrocytogenesis in Mouse Neural Progenitors. J Mol Biol, 2020
Photoactivatable RNA N6 -Methyladenosine Editing with CRISPR-Cas13. Small, 2020
Dual Function of PI(4,5)P2 in Insulin-Regulated Exocytic Trafficking of GLUT4 in Adipocytes. J Mol Biol, 2020
CofActor: A light- and stress-gated optogenetic clustering tool to study disease-associated cytoskeletal dynamics in living cells. J Biol Chem, 2020
Optical Activation of TrkB Signaling. J Mol Biol, 2020
CLIC4 is a cytokinetic cleavage furrow protein that regulates cortical cytoskeleton stability during cell division. J Cell Sci, 2020
Flotillin-upregulation acts as an epithelial-mesenchymal transition driver by promoting sphingosine kinase 2-dependent AXL stabilization. bioRxiv, 2020
Photoactivatable Cre recombinase 3.0 for in vivo mouse applications. Nat Commun, 2020
Optogenetic stimulation of phosphoinositides reveals a critical role of primary cilia in eye pressure regulation. Sci Adv, 2020
Phosphatidylinositol 4,5-bisphosphate directly interacts with the β and γ subunits of the sodium channel ENaC. J Biol Chem, 2020
A Single-Objective Light-Sheet Microscope with 200 nm-Scale Resolution. bioRxiv, 2020
Exosome-based delivery of super-repressor IκBα relieves sepsis-associated organ damage and mortality. Sci Adv, 2020
A Generalizable Optogenetic Strategy to Regulate Receptor Tyrosine Kinases during Vertebrate Embryonic Development. J Mol Biol, 2020
Cytokinetic bridge triggers de novo lumen formation in vivo. Nat Commun, 2020
Tissue-Scale Mechanical Coupling Reduces Morphogenetic Noise to Ensure Precision during Epithelial Folding. Dev Cell, 2020
Spatiotemporal control of phosphatidic acid signaling with optogenetic, engineered phospholipase Ds. J Cell Biol, 2020
Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling. Nat Commun, 2020
Engineering light-controllable CAR T cells for cancer immunotherapy. Sci Adv, 2020
Pulsatile MAPK Signaling Modulates p53 Activity to Control Cell Fate Decisions at the G2 Checkpoint for DNA Damage. Cell Rep, 2020
Optogenetic control of mRNA localization and translation in live cells. Nat Cell Biol, 2020
Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy. Sci Rep, 2020
Optogenetics reveals Cdc42 local activation by scaffold-mediated positive feedback and Ras GTPase. PLoS Biol, 2020
Shape-morphing living composites. Sci Adv, 2020
Tunable light and drug induced depletion of target proteins. Nat Commun, 2020
Stick-slip dynamics of cell adhesion triggers spontaneous symmetry breaking and directional migration of mesenchymal cells on one-dimensional lines. Sci Adv, 2020
ERK-mediated mechanochemical waves direct collective cell polarization. bioRxiv, 2019
Engineered BRET-Based Biologic Light Sources Enable Spatiotemporal Control over Diverse Optogenetic Systems. ACS Synth Biol, 2019
Primary Cilia Signaling Promotes Axonal Tract Development and Is Disrupted in Joubert Syndrome-Related Disorders Models. Dev Cell, 2019
Optical activation of TrkB receptors. bioRxiv, 2019
Directed evolution improves the catalytic efficiency of TEV protease. Nat Methods, 2019
Blue light-triggered optogenetic system for treating uveal melanoma. Oncogene, 2019
CofActor: A light and redox-gated optogenetic clustering tool to study abnormal cytoskeletal dynamics in stressed cells. bioRxiv, 2019
A yeast optogenetic toolkit (yOTK) for gene expression control in Saccharomyces cerevisiae. Biotechnol Bioeng, 2019
Optogenetic inhibition of Delta reveals digital Notch signaling output during tissue differentiation. EMBO Rep, 2019
Imaging of Morphological and Biochemical Hallmarks of Apoptosis with Optimized Optogenetic Actuators. PLoS ONE, 2019
Flotillins promote T cell receptor sorting through a fast Rab5-Rab11 endocytic recycling axis. Nat Commun, 2019
Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat Biotechnol, 2019
FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics. Nat Methods, 2019
Secretory Vesicle Clustering in Fungal Filamentous Cells Does Not Require Directional Growth. Cell Rep, 2019
Light-Induced Transcription Activation for Time-Lapse Microscopy Experiments in Living Cells. Methods Mol Biol, 2019
Transient Activations of Rac1 at the Lamellipodium Tip Trigger Membrane Protrusion. Curr Biol, 2019
Optogenetic gene editing in regional skin. Cell Res, 2019
Achieving tight control of a photoactivatable Cre recombinase gene switch: new design strategies and functional characterization in mammalian cells and rodent. Nucleic Acids Res, 2019
Light-Induced Protein Clustering for Optogenetic Interference and Protein Interaction Analysis in Drosophila S2 Cells. Biomolecules, 2019
Near-infrared light remotely up-regulate autophagy with spatiotemporal precision via upconversion optogenetic nanosystem. Biomaterials, 2019
Noninvasive optical activation of Flp recombinase for genetic manipulation in deep mouse brain regions. Nat Commun, 2019
Blue Light Switchable Cell–Cell Interactions Provide
Reversible and Spatiotemporal Control Towards
Bottom-Up Tissue Engineering. Adv Biosyst, 2019
Enhanced intrinsic CYP3A4 activity in human hepatic C3A cells with optically controlled CRISPR/dCas9 activator complex. Integr Biol (Camb), 2018
Development of a Wireless-Controlled LED Array for the Tunable Optogenetic Control of Cellular Activities. Engineering, 2018
Optogenetic dissection of Rac1 and Cdc42 gradient shaping. Nat Commun, 2018
Downregulation of basal myosin-II is required for cell shape changes and tissue invagination. EMBO J, 2018
Membrane dynamics induced by a PIP3 optogenetic tool. Anal Sci, 2018
Real-Time Genetic Compensation Defines the Dynamic Demands of Feedback Control. Cell, 2018
RalB directly triggers invasion downstream Ras by mobilizing the Wave complex. Elife, 2018
Light Control of the Tet Gene Expression System in Mammalian Cells. Cell Rep, 2018
Optogenetic control of epithelial-mesenchymal transition in cancer cells. Sci Rep, 2018
A Single-Component Optogenetic System Allows Stringent Switch of Gene Expression in Yeast Cells. ACS Synth Biol, 2018
Spatiotemporal control of zebrafish (Danio rerio) gene expression using a light-activated CRISPR activation system. Gene, 2018
Optical activation of TrkA signaling. ACS Synth Biol, 2018
Synthetic far-red light-mediated CRISPR-dCas9 device for inducing functional neuronal differentiation. Proc Natl Acad Sci USA, 2018
Guided morphogenesis through optogenetic activation of Rho signalling during early Drosophila embryogenesis. Nat Commun, 2018
Independent Control over Multiple Cell Types in Space and Time Using Orthogonal Blue and Red Light Switchable Cell Interactions. Adv Sci, 2018
A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging. Sci Rep, 2018
Regulation of cell cycle progression by cell-cell and cell-matrix forces. Nat Cell Biol, 2018
Optogenetic inhibition of Gαq protein signaling reduces calcium oscillation stochasticity. ACS Synth Biol, 2018
A mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation. Nat Commun, 2018
Analysis of the CaMKIIα and β splice-variant distribution among brain regions reveals isoform-specific differences in holoenzyme formation. Sci Rep, 2018
A biochemical network controlling basal myosin oscillation. Nat Commun, 2018
Optogenetic Control of Cell Migration. Methods Mol Biol, 2018
Light-activated protein interaction with high spatial subcellular confinement. Proc Natl Acad Sci USA, 2018
Generation of Optogenetically Modified Adenovirus Vector for Spatiotemporally Controllable Gene Therapy. ACS Chem Biol, 2018
Unique Roles of β-Arrestin in GPCR Trafficking Revealed by Photoinducible Dimerizers. Sci Rep, 2018
Spatiotemporal Control of TGF-β Signaling with Light. ACS Synth Biol, 2017
Gradients of Rac1 Nanoclusters Support Spatial Patterns of Rac1 Signaling. Cell Rep, 2017
Real-time observation of light-controlled transcription in living cells. J Cell Sci, 2017
Optogenetic regulation of artificial microRNA improves H2 production in green alga Chlamydomonas reinhardtii. Biotechnol Biofuels, 2017
Propagating Wave of ERK Activation Orients Collective Cell Migration. Dev Cell, 2017
Near-Infrared Light Triggered Upconversion Optogenetic Nanosystem for Cancer Therapy. ACS Nano, 2017
Design and Profiling of a Subcellular Targeted Optogenetic cAMP-Dependent Protein Kinase. Cell Chem Biol, 2017
Optimized light-inducible transcription in mammalian cells using Flavin Kelch-repeat F-box1/GIGANTEA and CRY2/CIB1. Nucleic Acids Res, 2017
A single-chain photoswitchable CRISPR-Cas9 architecture for light-inducible gene editing and transcription. ACS Chem Biol, 2017
Understanding CRY2 interactions for optical control of intracellular signaling. Nat Commun, 2017
CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation. Nat Methods, 2017
Two independent but synchronized Gβγ subunit-controlled pathways are essential for trailing-edge retraction during macrophage migration. J Biol Chem, 2017
Photo-Activatable Akt Probe - A New Tool to Study the Akt-Dependent Physiopathology of Cancer Cells. Oncol Res, 2017
An Engineered Optogenetic Switch for Spatiotemporal Control of Gene Expression, Cell Differentiation, and Tissue Morphogenesis. ACS Synth Biol, 2017
Vesicle Docking Is a Key Target of Local PI(4,5)P2 Metabolism in the Secretory Pathway of INS-1 Cells. Cell Rep, 2017
A calcium- and light-gated switch to induce gene expression in activated neurons. Nat Biotechnol, 2017
Optogenetic activation of Plexin-B1 reveals contact repulsion between osteoclasts and osteoblasts. Nat Commun, 2017
Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development. J Vis Exp, 2017
Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Nucleic Acids Res, 2017
Cell-matrix adhesion and cell-cell adhesion differentially control basal myosin oscillation and Drosophila egg chamber elongation. Nat Commun, 2017
Light-Regulated Protein Kinases Based on the CRY2-CIB1 System. Methods Mol Biol, 2017
Optogenetic control of cellular forces and mechanotransduction. Nat Commun, 2017
Epigenetic Editing of Ascl1 Gene in Neural Stem Cells by Optogenetics. Sci Rep, 2017
Optogenetic Control of Synaptic Composition and Function. Neuron, 2017
Transcription activator-like effector-mediated regulation of gene expression based on the inducible packaging and delivery via designed extracellular vesicles. Biochem Biophys Res Commun, 2017
A Photoactivatable Innate Immune Receptor for Optogenetic Inflammation. ACS Chem Biol, 2016
Plasma Membrane Association but Not Midzone Recruitment of RhoGEF ECT2 Is Essential for Cytokinesis. Cell Rep, 2016
Optogenetic inhibition of apical constriction during Drosophila embryonic development. Methods Cell Biol, 2016
An open-hardware platform for optogenetics and photobiology. Sci Rep, 2016
A photoactivatable Cre-loxP recombination system for optogenetic genome engineering. Nat Chem Biol, 2016
Reversible optogenetic control of kinase activity during differentiation and embryonic development. Development, 2016
Optogenetic regulation of site-specific subtelomeric DNA methylation. Oncotarget, 2016
Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein-protein interaction module. Nat Commun, 2016
Using HEK293T Expression System to Study Photoactive Plant Cryptochromes. Front Plant Sci, 2016
The Timing of Raf/ERK and AKT Activation in Protecting PC12 Cells against Oxidative Stress. PLoS ONE, 2016
Optogenetic activation reveals distinct roles of PIP3 and Akt in adipocyte insulin action. J Cell Sci, 2016
Optogenetic apoptosis: light-triggered cell death. Angew Chem Int Ed Engl, 2015
Photoactivatable CRISPR-Cas9 for optogenetic genome editing. Nat Biotechnol, 2015
The Dual Characteristics of Light-Induced Cryptochrome 2, Homo-oligomerization and Heterodimerization, for Optogenetic Manipulation in Mammalian Cells. ACS Synth Biol, 2015
Optogenetic control of molecular motors and organelle distributions in cells. Chem Biol, 2015
A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol, 2015
Optogenetic control of organelle transport and positioning. Nature, 2015
Single-molecule tracking of small GTPase Rac1 uncovers spatial regulation of membrane translocation and mechanism for polarized signaling. Proc Natl Acad Sci USA, 2015
Benchmarking of optical dimerizer systems. ACS Synth Biol, 2014
Engineered UV-A light-responsive gene expression system for measuring sun cream efficacy in mammalian cell culture. J Biotechnol, 2014
Optogenetic engineering: light-directed cell motility. Angew Chem Int Ed Engl, 2014
Subcellular optogenetic inhibition of G proteins generates signaling gradients and cell migration. Mol Biol Cell, 2014