CRY2/CIB1

Photoreceptor CRY2
Binding partner CIB1
Cofactor FAD
Source organism Arabidopsis thaliana
Mode of action heterodimerization
Excitation wavelength 450 nm
Reversion wavelength dark
Excitation time seconds
Reversion time minutes
Crystal structure of CRY2, without CIB1. (PDB ID: 6K8I)

Related original publications

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  1. A single-chain and fast-responding light-inducible Cre recombinase as a novel optogenetic switch. Elife, 2021
  2. Optical regulation of endogenous RhoA reveals switching of cellular responses by signal amplitude. bioRxiv, 2021
  3. Transient light-activated gene expression in Chinese hamster ovary cells. BMC Biotechnol, 2021
  4. A CRISPR-Cas9-Based Near-Infrared Upconversion-Activated DNA Methylation Editing System. ACS Appl Mater Interfaces, 2021
  5. Transcription activation is enhanced by multivalent interactions independent of liquid-liquid phase separation. bioRxiv, 2021
  6. A synthetic BRET-based optogenetic device for pulsatile transgene expression enabling glucose homeostasis in mice. Nat Commun, 2021
  7. Control of SRC molecular dynamics encodes distinct cytoskeletal responses by specifying signaling pathway usage. J Cell Sci, 2021
  8. Optogenetic Control of Phosphatidylinositol (3,4,5)‐triphosphate Production by Light‐sensitive Cryptochrome Proteins on the Plasma Membrane. Chin J Chem, 2021
  9. Random sub-diffusion and capture of genes by the nuclear pore reduces dynamics and coordinates interchromosomal movement. bioRxiv, 2021
  10. Optogenetic control of small GTPases reveals RhoA mediates intracellular calcium signaling. J Biol Chem, 2021
  11. Synthetic gene networks recapitulate dynamic signal decoding and differential gene expression. bioRxiv, 2021
  12. A Light-Inducible Split-dCas9 System for Inhibiting the Progression of Bladder Cancer Cells by Activating p53 and E-cadherin. Front Mol Biosci, 2021
  13. Liquid-liquid phase separation of light-inducible transcription factors increases transcription activation in mammalian cells and mice. Sci Adv, 2021
  14. Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse. Proc Natl Acad Sci U S A, 2020
  15. Regulating enzymatic reactions in Escherichia coli utilizing light-responsive cellular compartments based on liquid-liquid phase separation. bioRxiv, 2020
  16. Spatio-temporal Control of ERK Pulse Frequency Coordinates Fate Decisions during Mammary Acinar Morphogenesis. bioRxiv, 2020
  17. Single-Protein Tracking to Study Protein Interactions During Integrin-Based Migration. Methods Mol Biol, 2020
  18. Light-inducible Deformation of Mitochondria in Live Cells. bioRxiv, 2020
  19. Mechanical competition alters the cellular interpretation of an endogenous genetic programme. bioRxiv, 2020
  20. Multichromatic Control of Signaling Pathways in Mammalian Cells. Adv Biosyst, 2020
  21. Optical control of ERK and AKT signaling promotes axon regeneration and functional recovery of PNS and CNS in Drosophila. Elife, 2020
  22. Control of Cell Migration Using Optogenetics. Methods Mol Biol, 2020
  23. Activation of Cdc42 GTPase upon CRY2-Induced Cortical Recruitment Is Antagonized by GAPs in Fission Yeast. Cells, 2020
  24. CL6mN: Rationally Designed Optogenetic Photoswitches with Tunable Dissociation Dynamics. ACS Synth Biol, 2020
  25. A non-invasive far-red light-induced split-Cre recombinase system for controllable genome engineering in mice. Nat Commun, 2020
  26. Photo-SNAP-tag, a Light-Regulated Chemical Labeling System. ACS Chem Biol, 2020
  27. Optogenetic Control of Gene Expression Using Cryptochrome 2 and a Light-Activated Degron. Methods Mol Biol, 2020
  28. βH-spectrin is required for ratcheting apical pulsatile constrictions during tissue invagination. EMBO Rep, 2020
  29. Early But Not Delayed Optogenetic RAF Activation Promotes Astrocytogenesis in Mouse Neural Progenitors. J Mol Biol, 2020
  30. Photoactivatable RNA N6 -Methyladenosine Editing with CRISPR-Cas13. Small, 2020
  31. Dual Function of PI(4,5)P2 in Insulin-Regulated Exocytic Trafficking of GLUT4 in Adipocytes. J Mol Biol, 2020
  32. Collective ERK/Akt activity waves orchestrate epithelial homeostasis by driving apoptosis-induced survival. bioRxiv, 2020
  33. CofActor: A light- and stress-gated optogenetic clustering tool to study disease-associated cytoskeletal dynamics in living cells. J Biol Chem, 2020
  34. Optical Activation of TrkB Signaling. J Mol Biol, 2020
  35. CLIC4 is a cytokinetic cleavage furrow protein that regulates cortical cytoskeleton stability during cell division. J Cell Sci, 2020
  36. Flotillin-upregulation acts as an epithelial-mesenchymal transition driver by promoting sphingosine kinase 2-dependent AXL stabilization. bioRxiv, 2020
  37. Photoactivatable Cre recombinase 3.0 for in vivo mouse applications. Nat Commun, 2020
  38. Optogenetic stimulation of phosphoinositides reveals a critical role of primary cilia in eye pressure regulation. Sci Adv, 2020
  39. Phosphatidylinositol 4,5-bisphosphate directly interacts with the β and γ subunits of the sodium channel ENaC. J Biol Chem, 2020
  40. A Single-Objective Light-Sheet Microscope with 200 nm-Scale Resolution. bioRxiv, 2020
  41. Exosome-based delivery of super-repressor IκBα relieves sepsis-associated organ damage and mortality. Sci Adv, 2020
  42. A Generalizable Optogenetic Strategy to Regulate Receptor Tyrosine Kinases during Vertebrate Embryonic Development. J Mol Biol, 2020
  43. Cytokinetic bridge triggers de novo lumen formation in vivo. Nat Commun, 2020
  44. Tissue-Scale Mechanical Coupling Reduces Morphogenetic Noise to Ensure Precision during Epithelial Folding. Dev Cell, 2020
  45. Spatiotemporal control of phosphatidic acid signaling with optogenetic, engineered phospholipase Ds. J Cell Biol, 2020
  46. Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling. Nat Commun, 2020
  47. Engineering light-controllable CAR T cells for cancer immunotherapy. Sci Adv, 2020
  48. Pulsatile MAPK Signaling Modulates p53 Activity to Control Cell Fate Decisions at the G2 Checkpoint for DNA Damage. Cell Rep, 2020
  49. Optogenetic control of mRNA localization and translation in live cells. Nat Cell Biol, 2020
  50. Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy. Sci Rep, 2020
  51. Optogenetics reveals Cdc42 local activation by scaffold-mediated positive feedback and Ras GTPase. PLoS Biol, 2020
  52. Shape-morphing living composites. Sci Adv, 2020
  53. Tunable light and drug induced depletion of target proteins. Nat Commun, 2020
  54. Stick-slip dynamics of cell adhesion triggers spontaneous symmetry breaking and directional migration of mesenchymal cells on one-dimensional lines. Sci Adv, 2020
  55. ERK-mediated mechanochemical waves direct collective cell polarization. bioRxiv, 2019
  56. Engineered BRET-Based Biologic Light Sources Enable Spatiotemporal Control over Diverse Optogenetic Systems. ACS Synth Biol, 2019
  57. Primary Cilia Signaling Promotes Axonal Tract Development and Is Disrupted in Joubert Syndrome-Related Disorders Models. Dev Cell, 2019
  58. Optical activation of TrkB receptors. bioRxiv, 2019
  59. Directed evolution improves the catalytic efficiency of TEV protease. Nat Methods, 2019
  60. Blue light-triggered optogenetic system for treating uveal melanoma. Oncogene, 2019
  61. CofActor: A light and redox-gated optogenetic clustering tool to study abnormal cytoskeletal dynamics in stressed cells. bioRxiv, 2019
  62. A yeast optogenetic toolkit (yOTK) for gene expression control in Saccharomyces cerevisiae. Biotechnol Bioeng, 2019
  63. Optogenetic inhibition of Delta reveals digital Notch signaling output during tissue differentiation. EMBO Rep, 2019
  64. Imaging of Morphological and Biochemical Hallmarks of Apoptosis with Optimized Optogenetic Actuators. PLoS ONE, 2019
  65. Flotillins promote T cell receptor sorting through a fast Rab5-Rab11 endocytic recycling axis. Nat Commun, 2019
  66. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat Biotechnol, 2019
  67. FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics. Nat Methods, 2019
  68. Secretory Vesicle Clustering in Fungal Filamentous Cells Does Not Require Directional Growth. Cell Rep, 2019
  69. Light-Induced Transcription Activation for Time-Lapse Microscopy Experiments in Living Cells. Methods Mol Biol, 2019
  70. Transient Activations of Rac1 at the Lamellipodium Tip Trigger Membrane Protrusion. Curr Biol, 2019
  71. Optogenetic gene editing in regional skin. Cell Res, 2019
  72. Achieving tight control of a photoactivatable Cre recombinase gene switch: new design strategies and functional characterization in mammalian cells and rodent. Nucleic Acids Res, 2019
  73. Cross-linker-mediated regulation of actin network organization controls tissue morphogenesis. J Cell Biol, 2019
  74. LADL: light-activated dynamic looping for endogenous gene expression control. Nat Methods, 2019
  75. OpEn-Tag-A Customizable Optogenetic Toolbox To Dissect Subcellular Signaling. ACS Synth Biol, 2019
  76. Mps1 releases Mad1 from nuclear pores to ensure a robust mitotic checkpoint and accurate chromosome segregation. bioRxiv, 2019
  77. Accurate manipulation of optogenetic proteins with wavelength tunable femtosecond laser system. J Appl Phys, 2019
  78. Reversible Optogenetic Control of Growth Factor Signaling During Cell Differentiation and Vertebrate Embryonic Development. OSA Technical Digest, 2019
  79. Self-Organized Nuclear Positioning Synchronizes the Cell Cycle in Drosophila Embryos. Cell, 2019
  80. Cortical mitochondria regulate insulin secretion by local Ca2+ buffering. J Cell Sci, 2019
  81. Advances in optogenetic regulation of gene expression in mammalian cells using cryptochrome 2 (CRY2). Methods, 2019
  82. Optogenetic perturbation of the biochemical pathways that control cell behavior. Meth Enzymol, 2019
  83. Chapter Ten - Design, construction, and validation of optogenetic proteins. Lancet Diabetes Endocrinol, 2019
  84. Physical Plasma Membrane Perturbation Using Subcellular Optogenetics Drives Integrin-Activated Cell Migration. ACS Synth Biol, 2019
  85. Light-Induced Protein Clustering for Optogenetic Interference and Protein Interaction Analysis in Drosophila S2 Cells. Biomolecules, 2019
  86. Near-infrared light remotely up-regulate autophagy with spatiotemporal precision via upconversion optogenetic nanosystem. Biomaterials, 2019
  87. Noninvasive optical activation of Flp recombinase for genetic manipulation in deep mouse brain regions. Nat Commun, 2019
  88. Blue Light Switchable Cell–Cell Interactions Provide Reversible and Spatiotemporal Control Towards Bottom-Up Tissue Engineering. Adv Biosyst, 2019
  89. Enhanced intrinsic CYP3A4 activity in human hepatic C3A cells with optically controlled CRISPR/dCas9 activator complex. Integr Biol (Camb), 2018
  90. Development of a Wireless-Controlled LED Array for the Tunable Optogenetic Control of Cellular Activities. Engineering, 2018
  91. Optogenetic dissection of Rac1 and Cdc42 gradient shaping. Nat Commun, 2018
  92. Downregulation of basal myosin-II is required for cell shape changes and tissue invagination. EMBO J, 2018
  93. Membrane dynamics induced by a PIP3 optogenetic tool. Anal Sci, 2018
  94. Real-Time Genetic Compensation Defines the Dynamic Demands of Feedback Control. Cell, 2018
  95. RalB directly triggers invasion downstream Ras by mobilizing the Wave complex. Elife, 2018
  96. Light Control of the Tet Gene Expression System in Mammalian Cells. Cell Rep, 2018
  97. Optogenetic control of epithelial-mesenchymal transition in cancer cells. Sci Rep, 2018
  98. A Single-Component Optogenetic System Allows Stringent Switch of Gene Expression in Yeast Cells. ACS Synth Biol, 2018
  99. Spatiotemporal control of zebrafish (Danio rerio) gene expression using a light-activated CRISPR activation system. Gene, 2018
  100. Optical activation of TrkA signaling. ACS Synth Biol, 2018
  101. Synthetic far-red light-mediated CRISPR-dCas9 device for inducing functional neuronal differentiation. Proc Natl Acad Sci USA, 2018
  102. Guided morphogenesis through optogenetic activation of Rho signalling during early Drosophila embryogenesis. Nat Commun, 2018
  103. Independent Control over Multiple Cell Types in Space and Time Using Orthogonal Blue and Red Light Switchable Cell Interactions. Adv Sci, 2018
  104. A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging. Sci Rep, 2018
  105. Regulation of cell cycle progression by cell-cell and cell-matrix forces. Nat Cell Biol, 2018
  106. Optogenetic inhibition of Gαq protein signaling reduces calcium oscillation stochasticity. ACS Synth Biol, 2018
  107. A mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation. Nat Commun, 2018
  108. Analysis of the CaMKIIα and β splice-variant distribution among brain regions reveals isoform-specific differences in holoenzyme formation. Sci Rep, 2018
  109. A biochemical network controlling basal myosin oscillation. Nat Commun, 2018
  110. Optogenetic Control of Cell Migration. Methods Mol Biol, 2018
  111. Light-activated protein interaction with high spatial subcellular confinement. Proc Natl Acad Sci USA, 2018
  112. Generation of Optogenetically Modified Adenovirus Vector for Spatiotemporally Controllable Gene Therapy. ACS Chem Biol, 2018
  113. Unique Roles of β-Arrestin in GPCR Trafficking Revealed by Photoinducible Dimerizers. Sci Rep, 2018
  114. Spatiotemporal Control of TGF-β Signaling with Light. ACS Synth Biol, 2017
  115. Gradients of Rac1 Nanoclusters Support Spatial Patterns of Rac1 Signaling. Cell Rep, 2017
  116. Real-time observation of light-controlled transcription in living cells. J Cell Sci, 2017
  117. Optogenetic regulation of artificial microRNA improves H2 production in green alga Chlamydomonas reinhardtii. Biotechnol Biofuels, 2017
  118. Propagating Wave of ERK Activation Orients Collective Cell Migration. Dev Cell, 2017
  119. Near-Infrared Light Triggered Upconversion Optogenetic Nanosystem for Cancer Therapy. ACS Nano, 2017
  120. Design and Profiling of a Subcellular Targeted Optogenetic cAMP-Dependent Protein Kinase. Cell Chem Biol, 2017
  121. Optimized light-inducible transcription in mammalian cells using Flavin Kelch-repeat F-box1/GIGANTEA and CRY2/CIB1. Nucleic Acids Res, 2017
  122. A single-chain photoswitchable CRISPR-Cas9 architecture for light-inducible gene editing and transcription. ACS Chem Biol, 2017
  123. Understanding CRY2 interactions for optical control of intracellular signaling. Nat Commun, 2017
  124. CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation. Nat Methods, 2017
  125. Two independent but synchronized Gβγ subunit-controlled pathways are essential for trailing-edge retraction during macrophage migration. J Biol Chem, 2017
  126. Photo-Activatable Akt Probe - A New Tool to Study the Akt-Dependent Physiopathology of Cancer Cells. Oncol Res, 2017
  127. An Engineered Optogenetic Switch for Spatiotemporal Control of Gene Expression, Cell Differentiation, and Tissue Morphogenesis. ACS Synth Biol, 2017
  128. Vesicle Docking Is a Key Target of Local PI(4,5)P2 Metabolism in the Secretory Pathway of INS-1 Cells. Cell Rep, 2017
  129. A calcium- and light-gated switch to induce gene expression in activated neurons. Nat Biotechnol, 2017
  130. Optogenetic activation of Plexin-B1 reveals contact repulsion between osteoclasts and osteoblasts. Nat Commun, 2017
  131. Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development. J Vis Exp, 2017
  132. Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Nucleic Acids Res, 2017
  133. Cell-matrix adhesion and cell-cell adhesion differentially control basal myosin oscillation and Drosophila egg chamber elongation. Nat Commun, 2017
  134. Light-Regulated Protein Kinases Based on the CRY2-CIB1 System. Methods Mol Biol, 2017
  135. Optogenetic control of cellular forces and mechanotransduction. Nat Commun, 2017
  136. Epigenetic Editing of Ascl1 Gene in Neural Stem Cells by Optogenetics. Sci Rep, 2017
  137. Optogenetic Control of Synaptic Composition and Function. Neuron, 2017
  138. Transcription activator-like effector-mediated regulation of gene expression based on the inducible packaging and delivery via designed extracellular vesicles. Biochem Biophys Res Commun, 2017
  139. A Photoactivatable Innate Immune Receptor for Optogenetic Inflammation. ACS Chem Biol, 2016
  140. Plasma Membrane Association but Not Midzone Recruitment of RhoGEF ECT2 Is Essential for Cytokinesis. Cell Rep, 2016
  141. Optogenetic inhibition of apical constriction during Drosophila embryonic development. Methods Cell Biol, 2016
  142. An open-hardware platform for optogenetics and photobiology. Sci Rep, 2016
  143. A photoactivatable Cre-loxP recombination system for optogenetic genome engineering. Nat Chem Biol, 2016
  144. Reversible optogenetic control of kinase activity during differentiation and embryonic development. Development, 2016
  145. Optogenetic regulation of site-specific subtelomeric DNA methylation. Oncotarget, 2016
  146. Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein-protein interaction module. Nat Commun, 2016
  147. Using HEK293T Expression System to Study Photoactive Plant Cryptochromes. Front Plant Sci, 2016
  148. The Timing of Raf/ERK and AKT Activation in Protecting PC12 Cells against Oxidative Stress. PLoS ONE, 2016
  149. Optogenetic activation reveals distinct roles of PIP3 and Akt in adipocyte insulin action. J Cell Sci, 2016
  150. Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase. Nat Chem Biol, 2016
  151. Optogenetic oligomerization of Rab GTPases regulates intracellular membrane trafficking. Nat Chem Biol, 2016
  152. Optogenetically controlled RAF to characterize BRAF and CRAF protein kinase inhibitors. Sci Rep, 2016
  153. Frequency and amplitude control of cortical oscillations by phosphoinositide waves. Nat Chem Biol, 2016
  154. An Optogenetic Method to Modulate Cell Contractility during Tissue Morphogenesis. Dev Cell, 2015
  155. Predictive Spatiotemporal Manipulation of Signaling Perturbations Using Optogenetics. Biophys J, 2015
  156. Correlating in Vitro and in Vivo Activities of Light-Inducible Dimers: A Cellular Optogenetics Guide. ACS Synth Biol, 2015
  157. An optogenetic system for interrogating the temporal dynamics of Akt. Sci Rep, 2015
  158. Optogenetic Control of Gene Expression in Drosophila. PLoS ONE, 2015
  159. Photo-activatable Cre recombinase regulates gene expression in vivo. Sci Rep, 2015
  160. Optogenetic apoptosis: light-triggered cell death. Angew Chem Int Ed Engl, 2015
  161. Photoactivatable CRISPR-Cas9 for optogenetic genome editing. Nat Biotechnol, 2015
  162. The Dual Characteristics of Light-Induced Cryptochrome 2, Homo-oligomerization and Heterodimerization, for Optogenetic Manipulation in Mammalian Cells. ACS Synth Biol, 2015
  163. Optogenetic control of molecular motors and organelle distributions in cells. Chem Biol, 2015
  164. A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol, 2015
  165. CRISPR-Cas9-based photoactivatable transcription system. Chem Biol, 2015
  166. Optogenetic control of organelle transport and positioning. Nature, 2015
  167. Single-molecule tracking of small GTPase Rac1 uncovers spatial regulation of membrane translocation and mechanism for polarized signaling. Proc Natl Acad Sci USA, 2015
  168. Benchmarking of optical dimerizer systems. ACS Synth Biol, 2014
  169. Engineered UV-A light-responsive gene expression system for measuring sun cream efficacy in mammalian cell culture. J Biotechnol, 2014
  170. Optogenetic engineering: light-directed cell motility. Angew Chem Int Ed Engl, 2014
  171. Subcellular optogenetic inhibition of G proteins generates signaling gradients and cell migration. Mol Biol Cell, 2014
  172. Light-inducible receptor tyrosine kinases that regulate neurotrophin signalling. Nat Commun, 2014
  173. Reversible protein inactivation by optogenetic trapping in cells. Nat Methods, 2014
  174. Bidirectional regulation of mRNA translation in mammalian cells by using PUF domains. Angew Chem Int Ed Engl, 2014
  175. Light-mediated kinetic control reveals the temporal effect of the Raf/MEK/ERK pathway in PC12 cell neurite outgrowth. PLoS ONE, 2014
  176. Real-time optogenetic control of intracellular protein concentration in microbial cell cultures. Integr Biol (Camb), 2014
  177. Stochastic ERK activation induced by noise and cell-to-cell propagation regulates cell density-dependent proliferation. Mol Cell, 2013
  178. Light-inducible activation of target mRNA translation in mammalian cells. Chem Commun (Camb), 2013
  179. Optical control of mammalian endogenous transcription and epigenetic states. Nature, 2013
  180. Optogenetic control of PIP3: PIP3 is sufficient to induce the actin-based active part of growth cones and is regulated via endocytosis. PLoS ONE, 2013
  181. Live imaging of multicolor-labeled cells in Drosophila. Development, 2013
  182. Optogenetic control of transcription in zebrafish. PLoS ONE, 2012
  183. Light-mediated control of DNA transcription in yeast. Methods, 2012
  184. Optogenetic control of phosphoinositide metabolism. Proc Natl Acad Sci USA, 2012
  185. Rapid blue-light-mediated induction of protein interactions in living cells. Nat Methods, 2010