CRY2/CIB1

Photoreceptor CRY2
Binding partner CIB1
Cofactor FAD
Source organism Arabidopsis thaliana
Mode of action heterodimerization
Excitation wavelength 450 nm
Reversion wavelength dark
Excitation time seconds
Reversion time minutes

Related original publications

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  1. Stick-slip dynamics of cell adhesion triggers spontaneous symmetry breaking and directional migration of mesenchymal cells on one-dimensional lines. Sci Adv, 2020
  2. Engineered BRET-Based Biologic Light Sources Enable Spatiotemporal Control over Diverse Optogenetic Systems. ACS Synth Biol, 2019
  3. Primary Cilia Signaling Promotes Axonal Tract Development and Is Disrupted in Joubert Syndrome-Related Disorders Models. Dev Cell, 2019
  4. Blue light-triggered optogenetic system for treating uveal melanoma. Oncogene, 2019
  5. A yeast optogenetic toolkit (yOTK) for gene expression control in Saccharomyces cerevisiae. Biotechnol Bioeng, 2019
  6. Optogenetic inhibition of Delta reveals digital Notch signaling output during tissue differentiation. EMBO Rep, 2019
  7. Imaging of Morphological and Biochemical Hallmarks of Apoptosis with Optimized Optogenetic Actuators. PLoS ONE, 2019
  8. Flotillins promote T cell receptor sorting through a fast Rab5-Rab11 endocytic recycling axis. Nat Commun, 2019
  9. FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics. Nat Methods, 2019
  10. Secretory Vesicle Clustering in Fungal Filamentous Cells Does Not Require Directional Growth. Cell Rep, 2019
  11. CLIC4, a new component of the cytokinetic ring, regulates actin cytoskeleton dynamics during the anaphase-to-telophase transition. bioRxiv, 2019
  12. Transient Activations of Rac1 at the Lamellipodium Tip Trigger Membrane Protrusion. Curr Biol, 2019
  13. Optogenetic gene editing in regional skin. Cell Res, 2019
  14. Optogenetics reveals Cdc42 local activation by scaffold-mediated positive feedback and Ras GTPase. bioRxiv, 2019
  15. Achieving tight control of a photoactivatable Cre recombinase gene switch: new design strategies and functional characterization in mammalian cells and rodent. Nucleic Acids Res, 2019
  16. Cross-linker-mediated regulation of actin network organization controls tissue morphogenesis. J Cell Biol, 2019
  17. LADL: light-activated dynamic looping for endogenous gene expression control. Nat Methods, 2019
  18. OpEn-Tag-A Customizable Optogenetic Toolbox To Dissect Subcellular Signaling. ACS Synth Biol, 2019
  19. A yeast optogenetic toolkit (yOTK) for gene expression control in Saccharomyces cerevisiae. bioRxiv, 2019
  20. Mps1 releases Mad1 from nuclear pores to ensure a robust mitotic checkpoint and accurate chromosome segregation. bioRxiv, 2019
  21. Accurate manipulation of optogenetic proteins with wavelength tunable femtosecond laser system. J Appl Phys, 2019
  22. Reversible Optogenetic Control of Growth Factor Signaling During Cell Differentiation and Vertebrate Embryonic Development. OSA Technical Digest, 2019
  23. Self-Organized Nuclear Positioning Synchronizes the Cell Cycle in Drosophila Embryos. Cell, 2019
  24. Cortical mitochondria regulate insulin secretion by local Ca2+ buffering. J Cell Sci, 2019
  25. Advances in optogenetic regulation of gene expression in mammalian cells using cryptochrome 2 (CRY2). Methods, 2019
  26. Optogenetic perturbation of the biochemical pathways that control cell behavior. Meth Enzymol, 2019
  27. Chapter Ten - Design, construction, and validation of optogenetic proteins. Lancet Diabetes Endocrinol, 2019
  28. Physical Plasma Membrane Perturbation Using Subcellular Optogenetics Drives Integrin-Activated Cell Migration. ACS Synth Biol, 2019
  29. Light-Induced Protein Clustering for Optogenetic Interference and Protein Interaction Analysis in Drosophila S2 Cells. Biomolecules, 2019
  30. Near-infrared light remotely up-regulate autophagy with spatiotemporal precision via upconversion optogenetic nanosystem. Biomaterials, 2019
  31. Noninvasive optical activation of Flp recombinase for genetic manipulation in deep mouse brain regions. Nat Commun, 2019
  32. Blue Light Switchable Cell–Cell Interactions Provide Reversible and Spatiotemporal Control Towards Bottom-Up Tissue Engineering. Adv Biosyst, 2019
  33. Enhanced intrinsic CYP3A4 activity in human hepatic C3A cells with optically controlled CRISPR/dCas9 activator complex. Integr Biol (Camb), 2018
  34. Development of a Wireless-Controlled LED Array for the Tunable Optogenetic Control of Cellular Activities. Engineering, 2018
  35. Optogenetic dissection of Rac1 and Cdc42 gradient shaping. Nat Commun, 2018
  36. Downregulation of basal myosin-II is required for cell shape changes and tissue invagination. EMBO J, 2018
  37. Membrane dynamics induced by a PIP3 optogenetic tool. Anal Sci, 2018
  38. Real-Time Genetic Compensation Defines the Dynamic Demands of Feedback Control. Cell, 2018
  39. RalB directly triggers invasion downstream Ras by mobilizing the Wave complex. Elife, 2018
  40. Light Control of the Tet Gene Expression System in Mammalian Cells. Cell Rep, 2018
  41. Optogenetic control of epithelial-mesenchymal transition in cancer cells. Sci Rep, 2018
  42. A Single-Component Optogenetic System Allows Stringent Switch of Gene Expression in Yeast Cells. ACS Synth Biol, 2018
  43. Spatiotemporal control of zebrafish (Danio rerio) gene expression using a light-activated CRISPR activation system. Gene, 2018
  44. Optical activation of TrkA signaling. ACS Synth Biol, 2018
  45. Synthetic far-red light-mediated CRISPR-dCas9 device for inducing functional neuronal differentiation. Proc Natl Acad Sci USA, 2018
  46. Guided morphogenesis through optogenetic activation of Rho signalling during early Drosophila embryogenesis. Nat Commun, 2018
  47. Independent Control over Multiple Cell Types in Space and Time Using Orthogonal Blue and Red Light Switchable Cell Interactions. Adv Sci, 2018
  48. A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging. Sci Rep, 2018
  49. Regulation of cell cycle progression by cell-cell and cell-matrix forces. Nat Cell Biol, 2018
  50. Optogenetic inhibition of Gαq protein signaling reduces calcium oscillation stochasticity. ACS Synth Biol, 2018
  51. A mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation. Nat Commun, 2018
  52. Analysis of the CaMKIIα and β splice-variant distribution among brain regions reveals isoform-specific differences in holoenzyme formation. Sci Rep, 2018
  53. A biochemical network controlling basal myosin oscillation. Nat Commun, 2018
  54. Optogenetic Control of Cell Migration. Methods Mol Biol, 2018
  55. Light-activated protein interaction with high spatial subcellular confinement. Proc Natl Acad Sci USA, 2018
  56. Generation of Optogenetically Modified Adenovirus Vector for Spatiotemporally Controllable Gene Therapy. ACS Chem Biol, 2018
  57. Unique Roles of β-Arrestin in GPCR Trafficking Revealed by Photoinducible Dimerizers. Sci Rep, 2018
  58. Spatiotemporal Control of TGF-β Signaling with Light. ACS Synth Biol, 2017
  59. Gradients of Rac1 Nanoclusters Support Spatial Patterns of Rac1 Signaling. Cell Rep, 2017
  60. Real-time observation of light-controlled transcription in living cells. J Cell Sci, 2017
  61. Optogenetic regulation of artificial microRNA improves H2 production in green alga Chlamydomonas reinhardtii. Biotechnol Biofuels, 2017
  62. Propagating Wave of ERK Activation Orients Collective Cell Migration. Dev Cell, 2017
  63. Near-Infrared Light Triggered Upconversion Optogenetic Nanosystem for Cancer Therapy. ACS Nano, 2017
  64. Design and Profiling of a Subcellular Targeted Optogenetic cAMP-Dependent Protein Kinase. Cell Chem Biol, 2017
  65. Optimized light-inducible transcription in mammalian cells using Flavin Kelch-repeat F-box1/GIGANTEA and CRY2/CIB1. Nucleic Acids Res, 2017
  66. A single-chain photoswitchable CRISPR-Cas9 architecture for light-inducible gene editing and transcription. ACS Chem Biol, 2017
  67. Understanding CRY2 interactions for optical control of intracellular signaling. Nat Commun, 2017
  68. CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation. Nat Methods, 2017
  69. Two independent but synchronized Gβγ subunit-controlled pathways are essential for trailing-edge retraction during macrophage migration. J Biol Chem, 2017
  70. Photo-Activatable Akt Probe - A New Tool to Study the Akt-Dependent Physiopathology of Cancer Cells. Oncol Res, 2017
  71. An Engineered Optogenetic Switch for Spatiotemporal Control of Gene Expression, Cell Differentiation, and Tissue Morphogenesis. ACS Synth Biol, 2017
  72. Vesicle Docking Is a Key Target of Local PI(4,5)P2 Metabolism in the Secretory Pathway of INS-1 Cells. Cell Rep, 2017
  73. A calcium- and light-gated switch to induce gene expression in activated neurons. Nat Biotechnol, 2017
  74. Optogenetic activation of Plexin-B1 reveals contact repulsion between osteoclasts and osteoblasts. Nat Commun, 2017
  75. Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development. J Vis Exp, 2017
  76. Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Nucleic Acids Res, 2017
  77. Cell-matrix adhesion and cell-cell adhesion differentially control basal myosin oscillation and Drosophila egg chamber elongation. Nat Commun, 2017
  78. Light-Regulated Protein Kinases Based on the CRY2-CIB1 System. Methods Mol Biol, 2017
  79. Optogenetic control of cellular forces and mechanotransduction. Nat Commun, 2017
  80. Epigenetic Editing of Ascl1 Gene in Neural Stem Cells by Optogenetics. Sci Rep, 2017
  81. Optogenetic Control of Synaptic Composition and Function. Neuron, 2017
  82. Transcription activator-like effector-mediated regulation of gene expression based on the inducible packaging and delivery via designed extracellular vesicles. Biochem Biophys Res Commun, 2017
  83. A Photoactivatable Innate Immune Receptor for Optogenetic Inflammation. ACS Chem Biol, 2016
  84. Plasma Membrane Association but Not Midzone Recruitment of RhoGEF ECT2 Is Essential for Cytokinesis. Cell Rep, 2016
  85. Optogenetic inhibition of apical constriction during Drosophila embryonic development. Methods Cell Biol, 2016
  86. An open-hardware platform for optogenetics and photobiology. Sci Rep, 2016
  87. A photoactivatable Cre-loxP recombination system for optogenetic genome engineering. Nat Chem Biol, 2016
  88. Reversible optogenetic control of kinase activity during differentiation and embryonic development. Development, 2016
  89. Optogenetic regulation of site-specific subtelomeric DNA methylation. Oncotarget, 2016
  90. Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein-protein interaction module. Nat Commun, 2016
  91. Using HEK293T Expression System to Study Photoactive Plant Cryptochromes. Front Plant Sci, 2016
  92. The Timing of Raf/ERK and AKT Activation in Protecting PC12 Cells against Oxidative Stress. PLoS ONE, 2016
  93. Optogenetic activation reveals distinct roles of PIP3 and Akt in adipocyte insulin action. J Cell Sci, 2016
  94. Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase. Nat Chem Biol, 2016
  95. Optogenetic oligomerization of Rab GTPases regulates intracellular membrane trafficking. Nat Chem Biol, 2016
  96. Optogenetically controlled RAF to characterize BRAF and CRAF protein kinase inhibitors. Sci Rep, 2016
  97. Frequency and amplitude control of cortical oscillations by phosphoinositide waves. Nat Chem Biol, 2016
  98. An Optogenetic Method to Modulate Cell Contractility during Tissue Morphogenesis. Dev Cell, 2015
  99. Predictive Spatiotemporal Manipulation of Signaling Perturbations Using Optogenetics. Biophys J, 2015
  100. Correlating in Vitro and in Vivo Activities of Light-Inducible Dimers: A Cellular Optogenetics Guide. ACS Synth Biol, 2015
  101. An optogenetic system for interrogating the temporal dynamics of Akt. Sci Rep, 2015
  102. Optogenetic Control of Gene Expression in Drosophila. PLoS ONE, 2015
  103. Photo-activatable Cre recombinase regulates gene expression in vivo. Sci Rep, 2015
  104. Optogenetic apoptosis: light-triggered cell death. Angew Chem Int Ed Engl, 2015
  105. Photoactivatable CRISPR-Cas9 for optogenetic genome editing. Nat Biotechnol, 2015
  106. The Dual Characteristics of Light-Induced Cryptochrome 2, Homo-oligomerization and Heterodimerization, for Optogenetic Manipulation in Mammalian Cells. ACS Synth Biol, 2015
  107. Optogenetic control of molecular motors and organelle distributions in cells. Chem Biol, 2015
  108. A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol, 2015
  109. CRISPR-Cas9-based photoactivatable transcription system. Chem Biol, 2015
  110. Optogenetic control of organelle transport and positioning. Nature, 2015
  111. Single-molecule tracking of small GTPase Rac1 uncovers spatial regulation of membrane translocation and mechanism for polarized signaling. Proc Natl Acad Sci USA, 2015
  112. Benchmarking of optical dimerizer systems. ACS Synth Biol, 2014
  113. Engineered UV-A light-responsive gene expression system for measuring sun cream efficacy in mammalian cell culture. J Biotechnol, 2014
  114. Optogenetic engineering: light-directed cell motility. Angew Chem Int Ed Engl, 2014
  115. Subcellular optogenetic inhibition of G proteins generates signaling gradients and cell migration. Mol Biol Cell, 2014
  116. Light-inducible receptor tyrosine kinases that regulate neurotrophin signalling. Nat Commun, 2014
  117. Reversible protein inactivation by optogenetic trapping in cells. Nat Methods, 2014
  118. Bidirectional regulation of mRNA translation in mammalian cells by using PUF domains. Angew Chem Int Ed Engl, 2014
  119. Light-mediated kinetic control reveals the temporal effect of the Raf/MEK/ERK pathway in PC12 cell neurite outgrowth. PLoS ONE, 2014
  120. Real-time optogenetic control of intracellular protein concentration in microbial cell cultures. Integr Biol (Camb), 2014
  121. Stochastic ERK activation induced by noise and cell-to-cell propagation regulates cell density-dependent proliferation. Mol Cell, 2013
  122. Light-inducible activation of target mRNA translation in mammalian cells. Chem Commun (Camb), 2013
  123. Optical control of mammalian endogenous transcription and epigenetic states. Nature, 2013
  124. Optogenetic control of PIP3: PIP3 is sufficient to induce the actin-based active part of growth cones and is regulated via endocytosis. PLoS ONE, 2013
  125. Live imaging of multicolor-labeled cells in Drosophila. Development, 2013
  126. Optogenetic control of transcription in zebrafish. PLoS ONE, 2012
  127. Light-mediated control of DNA transcription in yeast. Methods, 2012
  128. Optogenetic control of phosphoinositide metabolism. Proc Natl Acad Sci USA, 2012
  129. Rapid blue-light-mediated induction of protein interactions in living cells. Nat Methods, 2010
  130. Light-Induced Transcription Activation for Time-Lapse Microscopy Experiments in Living Cells. Methods Mol Biol, 2019