PhyB/PIF3 & PhyB/PIF6

Photoreceptor PhyB
Binding partner PIF3 or PIF6
Cofactor PCB
Source organism Arabidopsis thaliana
Mode of action heterodimerization
Excitation wavelength 660 nm
Reversion wavelength 740 nm
Excitation time milliseconds
Reversion time milliseconds
Crystal structure of the PhyB photoreceptor, without PIF. (PDB ID: 4OUR)

Related original publications

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  1. Optogenetic control of cell signaling with red/far-red light-responsive optogenetic tools in Caenorhabditis elegans. bioRxiv, 2022
  2. Integration of light and temperature sensing by liquid-liquid phase separation of phytochrome B. Mol Cell, 2022
  3. Light-inducible T cell engagers trigger, tune and shape the activation of primary T cells. bioRxiv, 2022
  4. OptoAssay - Light-controlled Dynamic Bioassay Using Optogenetic Switches. bioRxiv, 2021
  5. Spatiotemporally confined red light-controlled gene delivery at single-cell resolution using adeno-associated viral vectors. Sci Adv, 2021
  6. Cross-TCR Antagonism Revealed by Optogenetically Tuning the Half-Life of the TCR Ligand Binding. Int J Mol Sci, 2021
  7. Photo-dependent membrane-less organelles formed from plant phyB and PIF6 proteins in mammalian cells. Int J Biol Macromol, 2021
  8. Liquid-liquid phase separation of light-inducible transcription factors increases transcription activation in mammalian cells and mice. Sci Adv, 2021
  9. Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse. Proc Natl Acad Sci U S A, 2020
  10. Improvement of Phycocyanobilin Synthesis for Genetically Encoded Phytochrome-Based Optogenetics. ACS Chem Biol, 2020
  11. Multichromatic Control of Signaling Pathways in Mammalian Cells. Adv Biosyst, 2020
  12. Optogenetic control of protein binding using light-switchable nanobodies. Nat Commun, 2020
  13. Optogenetic control of heterologous metabolism in E. coli. ACS Synth Biol, 2020
  14. CreLite: An Optogenetically Controlled Cre/loxP System Using Red Light. Dev Dyn, 2020
  15. Optogenetic Rescue of a Patterning Mutant. Curr Biol, 2020
  16. Optogenetic control of gene expression in plants in the presence of ambient white light. Nat Methods, 2020
  17. Development of optogenetic tools to manipulate cell cycle checkpoints. bioRxiv, 2020
  18. PhotoGal4: A Versatile Light-Dependent Switch for Spatiotemporal Control of Gene Expression in Drosophila Explants. iScience, 2020
  19. Optogenetic Tuning of Ligand Binding to The Human T cell Receptor Using The opto-ligand-TCR System. Bio Protoc, 2020
  20. Production, Purification and Characterization of Recombinant Biotinylated Phytochrome B for Extracellular Optogenetics. Bio Protoc, 2020
  21. Deconstructing and repurposing the light-regulated interplay between Arabidopsis phytochromes and interacting factors. Commun Biol, 2019
  22. Red/Far-Red Light Switchable Cargo Attachment and Release in Bacteria-Driven Microswimmers. Adv Healthc Mater, 2019
  23. Production of Phytochromes by High-Cell-Density E. coli Fermentation. ACS Synth Biol, 2019
  24. High-throughput multicolor optogenetics in microwell plates. Nat Protoc, 2019
  25. Optogenomic Interfaces: Bridging Biological Networks With the Electronic Digital World. IEEE, 2019
  26. Optogenetic control shows that kinetic proofreading regulates the activity of the T cell receptor. Elife, 2019
  27. Light-Controlled Affinity Purification of Protein Complexes Exemplified by the Resting ZAP70 Interactome. Front Immunol, 2019
  28. Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos. Methods Mol Biol, 2019
  29. Optogenetic control of integrin-matrix interaction. Commun Biol, 2019
  30. A size-invariant bud-duration timer enables robustness in yeast cell size control. PLoS ONE, 2018
  31. Light-Induced Printing of Protein Structures on Membranes in Vitro. Nano Lett, 2018
  32. Cancer mutations and targeted drugs can disrupt dynamic signal encoding by the Ras-Erk pathway. Science, 2018
  33. Generic and reversible opto-trapping of biomolecules. Acta Biomater, 2018
  34. Four Key Steps Control Glycolytic Flux in Mammalian Cells. Cell Syst, 2018
  35. Independent Control over Multiple Cell Types in Space and Time Using Orthogonal Blue and Red Light Switchable Cell Interactions. Adv Sci, 2018
  36. Light-controllable Transcription System by Nucleocytoplasmic Shuttling of a Truncated Phytochrome B. Photochem Photobiol, 2018
  37. L-SCRaMbLE as a tool for light-controlled Cre-mediated recombination in yeast. Nat Commun, 2018
  38. Synthetic Biology Makes Polymer Materials Count. Adv Mater, 2018
  39. Biosynthesis of Orthogonal Molecules Using Ferredoxin and Ferredoxin-NADP+ Reductase Systems Enables Genetically Encoded PhyB Optogenetics. ACS Synth Biol, 2018
  40. Efficient synthesis of phycocyanobilin in mammalian cells for optogenetic control of cell signaling. Proc Natl Acad Sci USA, 2017
  41. Tracing Information Flow from Erk to Target Gene Induction Reveals Mechanisms of Dynamic and Combinatorial Control. Mol Cell, 2017
  42. PhiReX: a programmable and red light-regulated protein expression switch for yeast. Nucleic Acids Res, 2017
  43. Optogenetic Control of Ras/Erk Signaling Using the Phy-PIF System. Methods Mol Biol, 2017
  44. A module for Rac temporal signal integration revealed with optogenetics. J Cell Biol, 2017
  45. A Phytochrome-Derived Photoswitch for Intracellular Transport. ACS Synth Biol, 2017
  46. The Spatiotemporal Limits of Developmental Erk Signaling. Dev Cell, 2017
  47. An open-hardware platform for optogenetics and photobiology. Sci Rep, 2016
  48. Optical manipulation of the alpha subunits of heterotrimeric G proteins using photoswitchable dimerization systems. Sci Rep, 2016
  49. A bacterial phytochrome-based optogenetic system controllable with near-infrared light. Nat Methods, 2016
  50. Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression. Methods Mol Biol, 2016
  51. Reversible Optogenetic Control of Subcellular Protein Localization in a Live Vertebrate Embryo. Dev Cell, 2016
  52. Signalling to the nucleus under the control of light and small molecules. Mol Biosyst, 2015
  53. Light-Activated Nuclear Translocation of Adeno-Associated Virus Nanoparticles Using Phytochrome B for Enhanced, Tunable, and Spatially Programmable Gene Delivery. ACS Nano, 2015
  54. Iterative experiment design guides the characterization of a light-inducible gene expression circuit. Proc Natl Acad Sci USA, 2015
  55. Probing Yeast Polarity with Acute, Reversible, Optogenetic Inhibition of Protein Function. ACS Synth Biol, 2015
  56. Red Light-Regulated Reversible Nuclear Localization of Proteins in Mammalian Cells and Zebrafish. ACS Synth Biol, 2015
  57. Benchmarking of optical dimerizer systems. ACS Synth Biol, 2014
  58. Orthogonal optogenetic triple-gene control in Mammalian cells. ACS Synth Biol, 2014
  59. Control of gene expression using a red- and far-red light-responsive bi-stable toggle switch. Nat Protoc, 2014
  60. A red light-controlled synthetic gene expression switch for plant systems. Mol Biosyst, 2014
  61. Using optogenetics to interrogate the dynamic control of signal transmission by the Ras/Erk module. Cell, 2013
  62. Synthesis of phycocyanobilin in mammalian cells. Chem Commun (Camb), 2013
  63. A light-inducible organelle-targeting system for dynamically activating and inactivating signaling in budding yeast. Mol Biol Cell, 2013
  64. Multi-chromatic control of mammalian gene expression and signaling. Nucleic Acids Res, 2013
  65. A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells. Nucleic Acids Res, 2013
  66. Light-mediated control of DNA transcription in yeast. Methods, 2012
  67. In silico feedback for in vivo regulation of a gene expression circuit. Nat Biotechnol, 2011
  68. Light-based feedback for controlling intracellular signaling dynamics. Nat Methods, 2011
  69. Light control of plasma membrane recruitment using the Phy-PIF system. Meth Enzymol, 2011
  70. Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature, 2009
  71. Genetically encoded photoswitching of actin assembly through the Cdc42-WASP-Arp2/3 complex pathway. Proc Natl Acad Sci USA, 2008
  72. Activation of protein splicing with light in yeast. Nat Methods, 2008
  73. A light-switchable gene promoter system. Nat Biotechnol, 2002
  74. Engineered Cas9 extracellular vesicles as a novel gene editing tool. J Extracell Vesicles, 2022
  75. Spatially Defined Gene Delivery into Native Cells with the Red Light-Controlled OptoAAV Technology. Curr Protoc, 2022