PhyB/PIF3 & PhyB/PIF6

Photoreceptor PhyB
Binding partner PIF3 or PIF6
Cofactor PCB
Source organism Arabidopsis thaliana
Mode of action heterodimerization
Excitation wavelength 660 nm
Reversion wavelength 740 nm
Excitation time milliseconds
Reversion time milliseconds
Crystal structure of the PhyB photoreceptor, without PIF. (PDB ID: 4OUR)

Related original publications

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  1. OptoREACT: Optogenetic Receptor Activation on Nonengineered Human T Cells. ACS Synth Biol, 2024
  2. Engineering of an Optogenetic T Cell Receptor Compatible with Fluorescence-Based Readouts. ACS Synth Biol, 2023
  3. Light-inducible T cell engagers trigger, tune, and shape the activation of primary T cells. Proc Natl Acad Sci U S A, 2023
  4. A biological camera that captures and stores images directly into DNA. Nat Commun, 2023
  5. Multidimensional characterization of inducible promoters and a highly light-sensitive LOV-transcription factor. Nat Commun, 2023
  6. A Photoreceptor-Based Hydrogel with Red Light-Responsive Reversible Sol-Gel Transition as Transient Cellular Matrix. Adv Mater Technol, 2023
  7. PhiReX 2.0: A Programmable and Red Light-Regulated CRISPR-dCas9 System for the Activation of Endogenous Genes in Saccharomyces cerevisiae. ACS Synth Biol, 2023
  8. Opto-APC: Engineering of cells that display phytochrome B on their surface for optogenetic studies of cell-cell interactions. Front Mol Biosci, 2023
  9. Optical Control of Cell Signaling with Red/Far-Red Light-Responsive Optogenetic Tools in Caenorhabditis elegans. ACS Synth Biol, 2023
  10. Orthogonal Light-Dependent Membrane Adhesion Induces Social Self-Sorting and Member-Specific DNA Communication in Synthetic Cell Communities. Small, 2023
  11. Integration of light and temperature sensing by liquid-liquid phase separation of phytochrome B. Mol Cell, 2022
  12. OptoAssay - Light-controlled Dynamic Bioassay Using Optogenetic Switches. bioRxiv, 2021
  13. Spatiotemporally confined red light-controlled gene delivery at single-cell resolution using adeno-associated viral vectors. Sci Adv, 2021
  14. Cross-TCR Antagonism Revealed by Optogenetically Tuning the Half-Life of the TCR Ligand Binding. Int J Mol Sci, 2021
  15. Photo-dependent membrane-less organelles formed from plant phyB and PIF6 proteins in mammalian cells. Int J Biol Macromol, 2021
  16. Liquid-liquid phase separation of light-inducible transcription factors increases transcription activation in mammalian cells and mice. Sci Adv, 2021
  17. Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse. Proc Natl Acad Sci U S A, 2020
  18. Improvement of Phycocyanobilin Synthesis for Genetically Encoded Phytochrome-Based Optogenetics. ACS Chem Biol, 2020
  19. Multichromatic Control of Signaling Pathways in Mammalian Cells. Adv Biosyst, 2020
  20. Optogenetic control of protein binding using light-switchable nanobodies. Nat Commun, 2020
  21. Optogenetic control of heterologous metabolism in E. coli. ACS Synth Biol, 2020
  22. CreLite: An Optogenetically Controlled Cre/loxP System Using Red Light. Dev Dyn, 2020
  23. Optogenetic Rescue of a Patterning Mutant. Curr Biol, 2020
  24. Optogenetic control of gene expression in plants in the presence of ambient white light. Nat Methods, 2020
  25. Development of optogenetic tools to manipulate cell cycle checkpoints. bioRxiv, 2020
  26. PhotoGal4: A Versatile Light-Dependent Switch for Spatiotemporal Control of Gene Expression in Drosophila Explants. iScience, 2020
  27. Optogenetic Tuning of Ligand Binding to The Human T cell Receptor Using The opto-ligand-TCR System. Bio Protoc, 2020
  28. Production, Purification and Characterization of Recombinant Biotinylated Phytochrome B for Extracellular Optogenetics. Bio Protoc, 2020
  29. Deconstructing and repurposing the light-regulated interplay between Arabidopsis phytochromes and interacting factors. Commun Biol, 2019
  30. Red/Far-Red Light Switchable Cargo Attachment and Release in Bacteria-Driven Microswimmers. Adv Healthc Mater, 2019
  31. Production of Phytochromes by High-Cell-Density E. coli Fermentation. ACS Synth Biol, 2019
  32. High-throughput multicolor optogenetics in microwell plates. Nat Protoc, 2019
  33. Optogenomic Interfaces: Bridging Biological Networks With the Electronic Digital World. IEEE, 2019
  34. Optogenetic control shows that kinetic proofreading regulates the activity of the T cell receptor. Elife, 2019
  35. Light-Controlled Affinity Purification of Protein Complexes Exemplified by the Resting ZAP70 Interactome. Front Immunol, 2019
  36. Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos. Methods Mol Biol, 2019
  37. Optogenetic control of integrin-matrix interaction. Commun Biol, 2019
  38. A size-invariant bud-duration timer enables robustness in yeast cell size control. PLoS ONE, 2018
  39. Light-Induced Printing of Protein Structures on Membranes in Vitro. Nano Lett, 2018
  40. Cancer mutations and targeted drugs can disrupt dynamic signal encoding by the Ras-Erk pathway. Science, 2018
  41. Generic and reversible opto-trapping of biomolecules. Acta Biomater, 2018
  42. Four Key Steps Control Glycolytic Flux in Mammalian Cells. Cell Syst, 2018
  43. Independent Control over Multiple Cell Types in Space and Time Using Orthogonal Blue and Red Light Switchable Cell Interactions. Adv Sci, 2018
  44. Light-controllable Transcription System by Nucleocytoplasmic Shuttling of a Truncated Phytochrome B. Photochem Photobiol, 2018
  45. L-SCRaMbLE as a tool for light-controlled Cre-mediated recombination in yeast. Nat Commun, 2018
  46. Synthetic Biology Makes Polymer Materials Count. Adv Mater, 2018
  47. Biosynthesis of Orthogonal Molecules Using Ferredoxin and Ferredoxin-NADP+ Reductase Systems Enables Genetically Encoded PhyB Optogenetics. ACS Synth Biol, 2018
  48. Efficient synthesis of phycocyanobilin in mammalian cells for optogenetic control of cell signaling. Proc Natl Acad Sci USA, 2017
  49. Tracing Information Flow from Erk to Target Gene Induction Reveals Mechanisms of Dynamic and Combinatorial Control. Mol Cell, 2017
  50. PhiReX: a programmable and red light-regulated protein expression switch for yeast. Nucleic Acids Res, 2017
  51. Optogenetic Control of Ras/Erk Signaling Using the Phy-PIF System. Methods Mol Biol, 2017
  52. A module for Rac temporal signal integration revealed with optogenetics. J Cell Biol, 2017
  53. A Phytochrome-Derived Photoswitch for Intracellular Transport. ACS Synth Biol, 2017
  54. The Spatiotemporal Limits of Developmental Erk Signaling. Dev Cell, 2017
  55. An open-hardware platform for optogenetics and photobiology. Sci Rep, 2016
  56. Optical manipulation of the alpha subunits of heterotrimeric G proteins using photoswitchable dimerization systems. Sci Rep, 2016
  57. A bacterial phytochrome-based optogenetic system controllable with near-infrared light. Nat Methods, 2016
  58. Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression. Methods Mol Biol, 2016
  59. Reversible Optogenetic Control of Subcellular Protein Localization in a Live Vertebrate Embryo. Dev Cell, 2016
  60. Signalling to the nucleus under the control of light and small molecules. Mol Biosyst, 2015
  61. Light-Activated Nuclear Translocation of Adeno-Associated Virus Nanoparticles Using Phytochrome B for Enhanced, Tunable, and Spatially Programmable Gene Delivery. ACS Nano, 2015
  62. Iterative experiment design guides the characterization of a light-inducible gene expression circuit. Proc Natl Acad Sci USA, 2015
  63. Probing Yeast Polarity with Acute, Reversible, Optogenetic Inhibition of Protein Function. ACS Synth Biol, 2015
  64. Red Light-Regulated Reversible Nuclear Localization of Proteins in Mammalian Cells and Zebrafish. ACS Synth Biol, 2015
  65. Benchmarking of optical dimerizer systems. ACS Synth Biol, 2014
  66. Orthogonal optogenetic triple-gene control in Mammalian cells. ACS Synth Biol, 2014
  67. Control of gene expression using a red- and far-red light-responsive bi-stable toggle switch. Nat Protoc, 2014
  68. A red light-controlled synthetic gene expression switch for plant systems. Mol Biosyst, 2014
  69. Using optogenetics to interrogate the dynamic control of signal transmission by the Ras/Erk module. Cell, 2013
  70. Synthesis of phycocyanobilin in mammalian cells. Chem Commun (Camb), 2013
  71. A light-inducible organelle-targeting system for dynamically activating and inactivating signaling in budding yeast. Mol Biol Cell, 2013
  72. Multi-chromatic control of mammalian gene expression and signaling. Nucleic Acids Res, 2013
  73. A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells. Nucleic Acids Res, 2013
  74. Light-mediated control of DNA transcription in yeast. Methods, 2012
  75. In silico feedback for in vivo regulation of a gene expression circuit. Nat Biotechnol, 2011
  76. Light-based feedback for controlling intracellular signaling dynamics. Nat Methods, 2011
  77. Light control of plasma membrane recruitment using the Phy-PIF system. Meth Enzymol, 2011
  78. Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature, 2009
  79. Genetically encoded photoswitching of actin assembly through the Cdc42-WASP-Arp2/3 complex pathway. Proc Natl Acad Sci USA, 2008
  80. Activation of protein splicing with light in yeast. Nat Methods, 2008
  81. A light-switchable gene promoter system. Nat Biotechnol, 2002
  82. Engineered Cas9 extracellular vesicles as a novel gene editing tool. J Extracell Vesicles, 2022
  83. Spatially Defined Gene Delivery into Native Cells with the Red Light-Controlled OptoAAV Technology. Curr Protoc, 2022