PhyB/PIF3 & PhyB/PIF6

Photoreceptor PhyB
Binding partner PIF3 or PIF6
Cofactor PCB
Source organism Arabidopsis thaliana
Mode of action heterodimerization
Excitation wavelength 660 nm
Reversion wavelength 740 nm
Excitation time milliseconds
Reversion time milliseconds
Crystal structure of the PhyB photoreceptor, without PIF. (PDB ID: 4OUR)

Related original publications

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  1. High-throughput multicolor optogenetics in microwell plates. Nat Protoc, 2019
  2. Optogenomic Interfaces: Bridging Biological Networks With the Electronic Digital World. IEEE, 2019
  3. Optogenetic control shows that kinetic proofreading regulates the activity of the T cell receptor. Elife, 2019
  4. Light-Controlled Affinity Purification of Protein Complexes Exemplified by the Resting ZAP70 Interactome. Front Immunol, 2019
  5. Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos. Methods Mol Biol, 2019
  6. Optogenetic control of integrin-matrix interaction. Commun Biol, 2019
  7. A size-invariant bud-duration timer enables robustness in yeast cell size control. PLoS ONE, 2018
  8. Light-Induced Printing of Protein Structures on Membranes in Vitro. Nano Lett, 2018
  9. Cancer mutations and targeted drugs can disrupt dynamic signal encoding by the Ras-Erk pathway. Science, 2018
  10. Generic and reversible opto-trapping of biomolecules. Acta Biomater, 2018
  11. Four Key Steps Control Glycolytic Flux in Mammalian Cells. Cell Syst, 2018
  12. Independent Control over Multiple Cell Types in Space and Time Using Orthogonal Blue and Red Light Switchable Cell Interactions. Adv Sci, 2018
  13. Light-controllable Transcription System by Nucleocytoplasmic Shuttling of a Truncated Phytochrome B. Photochem Photobiol, 2018
  14. L-SCRaMbLE as a tool for light-controlled Cre-mediated recombination in yeast. Nat Commun, 2018
  15. Synthetic Biology Makes Polymer Materials Count. Adv Mater, 2018
  16. Biosynthesis of Orthogonal Molecules Using Ferredoxin and Ferredoxin-NADP+ Reductase Systems Enables Genetically Encoded PhyB Optogenetics. ACS Synth Biol, 2018
  17. Efficient synthesis of phycocyanobilin in mammalian cells for optogenetic control of cell signaling. Proc Natl Acad Sci USA, 2017
  18. Tracing Information Flow from Erk to Target Gene Induction Reveals Mechanisms of Dynamic and Combinatorial Control. Mol Cell, 2017
  19. PhiReX: a programmable and red light-regulated protein expression switch for yeast. Nucleic Acids Res, 2017
  20. Optogenetic Control of Ras/Erk Signaling Using the Phy-PIF System. Methods Mol Biol, 2017
  21. A module for Rac temporal signal integration revealed with optogenetics. J Cell Biol, 2017
  22. A Phytochrome-Derived Photoswitch for Intracellular Transport. ACS Synth Biol, 2017
  23. The Spatiotemporal Limits of Developmental Erk Signaling. Dev Cell, 2017
  24. An open-hardware platform for optogenetics and photobiology. Sci Rep, 2016
  25. Optical manipulation of the alpha subunits of heterotrimeric G proteins using photoswitchable dimerization systems. Sci Rep, 2016
  26. A bacterial phytochrome-based optogenetic system controllable with near-infrared light. Nat Methods, 2016
  27. Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression. Methods Mol Biol, 2016
  28. Reversible Optogenetic Control of Subcellular Protein Localization in a Live Vertebrate Embryo. Dev Cell, 2016
  29. Signalling to the nucleus under the control of light and small molecules. Mol Biosyst, 2015
  30. Light-Activated Nuclear Translocation of Adeno-Associated Virus Nanoparticles Using Phytochrome B for Enhanced, Tunable, and Spatially Programmable Gene Delivery. ACS Nano, 2015
  31. Iterative experiment design guides the characterization of a light-inducible gene expression circuit. Proc Natl Acad Sci USA, 2015
  32. Probing Yeast Polarity with Acute, Reversible, Optogenetic Inhibition of Protein Function. ACS Synth Biol, 2015
  33. Red Light-Regulated Reversible Nuclear Localization of Proteins in Mammalian Cells and Zebrafish. ACS Synth Biol, 2015
  34. Benchmarking of optical dimerizer systems. ACS Synth Biol, 2014
  35. Orthogonal optogenetic triple-gene control in Mammalian cells. ACS Synth Biol, 2014
  36. Control of gene expression using a red- and far-red light-responsive bi-stable toggle switch. Nat Protoc, 2014
  37. A red light-controlled synthetic gene expression switch for plant systems. Mol Biosyst, 2014
  38. Using optogenetics to interrogate the dynamic control of signal transmission by the Ras/Erk module. Cell, 2013
  39. Synthesis of phycocyanobilin in mammalian cells. Chem Commun (Camb), 2013
  40. A light-inducible organelle-targeting system for dynamically activating and inactivating signaling in budding yeast. Mol Biol Cell, 2013
  41. Multi-chromatic control of mammalian gene expression and signaling. Nucleic Acids Res, 2013
  42. A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells. Nucleic Acids Res, 2013
  43. Light-mediated control of DNA transcription in yeast. Methods, 2012
  44. In silico feedback for in vivo regulation of a gene expression circuit. Nat Biotechnol, 2011
  45. Light-based feedback for controlling intracellular signaling dynamics. Nat Methods, 2011
  46. Light control of plasma membrane recruitment using the Phy-PIF system. Meth Enzymol, 2011
  47. Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature, 2009
  48. Genetically encoded photoswitching of actin assembly through the Cdc42-WASP-Arp2/3 complex pathway. Proc Natl Acad Sci USA, 2008
  49. Activation of protein splicing with light in yeast. Nat Methods, 2008
  50. A light-switchable gene promoter system. Nat Biotechnol, 2002