PhyB/PIF3 & PhyB/PIF6

Photoreceptor PhyB
Binding partner PIF3 or PIF6
Cofactor PCB
Source organism Arabidopsis thaliana
Mode of action heterodimerization
Excitation wavelength 660 nm
Reversion wavelength 740 nm
Excitation time milliseconds
Reversion time milliseconds
Crystal structure of the PhyB photoreceptor, without PIF. (PDB ID: 4OUR)

Related original publications

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  1. Cancer mutations and targeted drugs can disrupt dynamic signal encoding by the Ras-Erk pathway. Science, 2018
  2. Generic and reversible opto-trapping of biomolecules. Acta Biomater, 2018
  3. Four Key Steps Control Glycolytic Flux in Mammalian Cells. Cell Syst, 2018
  4. Independent Control over Multiple Cell Types in Space and Time Using Orthogonal Blue and Red Light Switchable Cell Interactions. Adv Sci, 2018
  5. Light-controllable Transcription System by Nucleocytoplasmic Shuttling of a Truncated Phytochrome B. Photochem Photobiol, 2018
  6. L-SCRaMbLE as a tool for light-controlled Cre-mediated recombination in yeast. Nat Commun, 2018
  7. Synthetic Biology Makes Polymer Materials Count. Adv Mater, 2018
  8. Biosynthesis of Orthogonal Molecules Using Ferredoxin and Ferredoxin-NADP+ Reductase Systems Enables Genetically Encoded PhyB Optogenetics. ACS Synth Biol, 2018
  9. Efficient synthesis of phycocyanobilin in mammalian cells for optogenetic control of cell signaling. Proc Natl Acad Sci USA, 2017
  10. Tracing Information Flow from Erk to Target Gene Induction Reveals Mechanisms of Dynamic and Combinatorial Control. Mol Cell, 2017
  11. PhiReX: a programmable and red light-regulated protein expression switch for yeast. Nucleic Acids Res, 2017
  12. Optogenetic Control of Ras/Erk Signaling Using the Phy-PIF System. Methods Mol Biol, 2017
  13. A module for Rac temporal signal integration revealed with optogenetics. J Cell Biol, 2017
  14. A Phytochrome-Derived Photoswitch for Intracellular Transport. ACS Synth Biol, 2017
  15. The Spatiotemporal Limits of Developmental Erk Signaling. Dev Cell, 2017
  16. An open-hardware platform for optogenetics and photobiology. Sci Rep, 2016
  17. Optical manipulation of the alpha subunits of heterotrimeric G proteins using photoswitchable dimerization systems. Sci Rep, 2016
  18. A bacterial phytochrome-based optogenetic system controllable with near-infrared light. Nat Methods, 2016
  19. Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression. Methods Mol Biol, 2016
  20. Reversible Optogenetic Control of Subcellular Protein Localization in a Live Vertebrate Embryo. Dev Cell, 2016
  21. Signalling to the nucleus under the control of light and small molecules. Mol Biosyst, 2015
  22. Light-Activated Nuclear Translocation of Adeno-Associated Virus Nanoparticles Using Phytochrome B for Enhanced, Tunable, and Spatially Programmable Gene Delivery. ACS Nano, 2015
  23. Iterative experiment design guides the characterization of a light-inducible gene expression circuit. Proc Natl Acad Sci USA, 2015
  24. Probing Yeast Polarity with Acute, Reversible, Optogenetic Inhibition of Protein Function. ACS Synth Biol, 2015
  25. Red Light-Regulated Reversible Nuclear Localization of Proteins in Mammalian Cells and Zebrafish. ACS Synth Biol, 2015
  26. Benchmarking of optical dimerizer systems. ACS Synth Biol, 2014
  27. Orthogonal optogenetic triple-gene control in Mammalian cells. ACS Synth Biol, 2014
  28. Control of gene expression using a red- and far-red light-responsive bi-stable toggle switch. Nat Protoc, 2014
  29. A red light-controlled synthetic gene expression switch for plant systems. Mol Biosyst, 2014
  30. Using optogenetics to interrogate the dynamic control of signal transmission by the Ras/Erk module. Cell, 2013
  31. Synthesis of phycocyanobilin in mammalian cells. Chem Commun (Camb), 2013
  32. A light-inducible organelle-targeting system for dynamically activating and inactivating signaling in budding yeast. Mol Biol Cell, 2013
  33. Multi-chromatic control of mammalian gene expression and signaling. Nucleic Acids Res, 2013
  34. A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells. Nucleic Acids Res, 2013
  35. Light-mediated control of DNA transcription in yeast. Methods, 2012
  36. In silico feedback for in vivo regulation of a gene expression circuit. Nat Biotechnol, 2011
  37. Light-based feedback for controlling intracellular signaling dynamics. Nat Methods, 2011
  38. Light control of plasma membrane recruitment using the Phy-PIF system. Meth Enzymol, 2011
  39. Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature, 2009
  40. Genetically encoded photoswitching of actin assembly through the Cdc42-WASP-Arp2/3 complex pathway. Proc Natl Acad Sci USA, 2008
  41. Activation of protein splicing with light in yeast. Nat Methods, 2008
  42. A light-switchable gene promoter system. Nat Biotechnol, 2002