LOVTRAP

Photoreceptor AsLOV2
Binding partner Zdk
Cofactor FMN
Source organism Avena sativa
Mode of action heterodimerization, dissociation
Excitation wavelength 450 nm
Reversion wavelength dark
Excitation time seconds
Reversion time seconds to minutes (variants available)
Crystal structure of the LOVTRAP heterodimer, with Zdk variant Zdk1. (PDB ID: 5EFW)

Related original publications

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  1. RudLOV—a new optically synchronized cargo transport method reveals unexpected effect of dynasore. bioRxiv, 2023
  2. Optogenetic cleavage of the Miro GTPase reveals the direct consequences of real-time loss of function in Drosophila. PLoS Biol, 2023
  3. Optogenetic strategies for optimizing the performance of biosensors of membrane phospholipids in live cells. bioRxiv, 2023
  4. Optogenetic control of kinesins -1, -2, -3 and dynein reveals their specific roles in vesicular transport. bioRxiv, 2023
  5. Transcription factor localization dynamics and DNA binding drive distinct promoter interpretations. Cell Rep, 2023
  6. Progressive enhancement of kinetic proofreading in T cell antigen discrimination from receptor activation to DAG generation. Elife, 2022
  7. Optogenetic control of RelA reveals effect of transcription factor dynamics on downstream gene expression. bioRxiv, 2022
  8. Optogenetic control of NOTCH1 signaling. Cell Commun Signal, 2022
  9. A novel mechanism of bulk cytoplasmic transport by cortical dynein in Drosophila ovary. Elife, 2022
  10. Optogenetic EB1 inactivation shortens metaphase spindles by disrupting cortical force-producing interactions with astral microtubules. Curr Biol, 2022
  11. Analysis of Three Architectures for Controlling PTP1B with Light. ACS Synth Biol, 2021
  12. Gezielte Injektion von Effektoren durch Kontrolle der Proteindynamik. BIOspektrum (Heidelb), 2021
  13. An Optogenetic Toolbox for Synergistic Regulation of Protein Abundance. ACS Synth Biol, 2021
  14. Desensitisation of Notch signalling through dynamic adaptation in the nucleus. EMBO J, 2021
  15. Light-Responsive Dynamic Protein Hydrogels Based on LOVTRAP. Langmuir, 2021
  16. An optogenetic method for interrogating YAP1 and TAZ nuclear-cytoplasmic shuttling. J Cell Sci, 2021
  17. Circularly permuted LOV2 as a modular photoswitch for optogenetic engineering. Nat Chem Biol, 2021
  18. Optogenetic Control of Non-Apoptotic Cell Death. Adv Biology, 2021
  19. Synthetic Protein Condensates That Inducibly Recruit and Release Protein Activity in Living Cells. J Am Chem Soc, 2021
  20. A synthetic BRET-based optogenetic device for pulsatile transgene expression enabling glucose homeostasis in mice. Nat Commun, 2021
  21. Engineering Supramolecular Organizing Centers for Optogenetic Control of Innate Immune Responses. Adv Biol, 2020
  22. The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics. J Cell Biol, 2020
  23. Optogenetic Tuning Reveals Rho Amplification-Dependent Dynamics of a Cell Contraction Signal Network. Cell Rep, 2020
  24. Optogenetic Control Reveals Differential Promoter Interpretation of Transcription Factor Nuclear Translocation Dynamics. Cell Syst, 2020
  25. Optogenetic Tuning of Protein-protein Binding in Bilayers Using LOVTRAP. Bio Protoc, 2020
  26. LITESEC-T3SS - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution. Nat Commun, 2020
  27. Actin waves transport RanGTP to the neurite tip to regulate non-centrosomal microtubules in neurons. J Cell Sci, 2020
  28. Optogenetics: Rho GTPases Activated by Light in Living Macrophages. Methods Mol Biol, 2020
  29. A Computational Protocol for Regulating Protein Binding Reactions with a Light-Sensitive Protein Dimer. J Mol Biol, 2019
  30. Optogenetic Control of Microtubule Dynamics. Methods Mol Biol, 2019
  31. Optogenetic control of cofilin and αTAT in living cells using Z-lock. Nat Chem Biol, 2019
  32. Using Tools from Optogenetics to Create Light-Responsive Biomaterials: LOVTRAP-PEG Hydrogels for Dynamic Peptide Immobilization. Ann Biomed Eng, 2019
  33. ESCRT-mediated phagophore sealing during mitophagy. Autophagy, 2019
  34. Optogenetic downregulation of protein levels with an ultrasensitive switch. ACS Synth Biol, 2019
  35. Light-based tuning of ligand half-life supports kinetic proofreading model of T cell signaling. Elife, 2019
  36. Luciferase-LOV BRET enables versatile and specific transcriptional readout of cellular protein-protein interactions. Elife, 2019
  37. Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET. Nat Chem Biol, 2018
  38. Rewiring Calcium Signaling for Precise Transcriptional Reprogramming. ACS Synth Biol, 2018
  39. Light-dependent cytoplasmic recruitment enhances the dynamic range of a nuclear import photoswitch. Chembiochem, 2018
  40. Local control of intracellular microtubule dynamics by EB1 photodissociation. Nat Cell Biol, 2018
  41. Discovery of long-range inhibitory signaling to ensure single axon formation. Nat Commun, 2017
  42. LOVTRAP: A Versatile Method to Control Protein Function with Light. Curr Protoc Cell Biol, 2016
  43. LOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods, 2016
  44. Quantifying persistence in the T-cell signaling network using an optically controllable antigen receptor. Mol Syst Biol, 2021
  45. Controlling the Potency of T Cell Activation Using an Optically Tunable Chimeric Antigen Receptor. Methods Mol Biol, 2024