LOVTRAP

Photoreceptor AsLOV2
Binding partner Zdk
Cofactor FMN
Source organism Avena sativa
Mode of action heterodimerization, dissociation
Excitation wavelength 450 nm
Reversion wavelength dark
Excitation time seconds
Reversion time seconds to minutes (variants available)
Crystal structure of the LOVTRAP heterodimer, with Zdk variant Zdk1. (PDB ID: 5EFW)

Related original publications

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  1. Desensitisation of Notch signalling through dynamic adaptation in the nucleus. EMBO J, 2021
  2. An optogenetic method for interrogating YAP1 and TAZ nuclear-cytoplasmic shuttling. J Cell Sci, 2021
  3. Circularly permuted LOV2 as a modular photoswitch for optogenetic engineering. Nat Chem Biol, 2021
  4. Optogenetic Control of Non-Apoptotic Cell Death. Adv Biology, 2021
  5. Synthetic Protein Condensates That Inducibly Recruit and Release Protein Activity in Living Cells. J Am Chem Soc, 2021
  6. A synthetic BRET-based optogenetic device for pulsatile transgene expression enabling glucose homeostasis in mice. Nat Commun, 2021
  7. Engineering Supramolecular Organizing Centers for Optogenetic Control of Innate Immune Responses. Adv Biol, 2020
  8. Synthetic protein condensates that recruit and release protein activity in living cells. bioRxiv, 2020
  9. Quantifying signal persistence in the T cell signaling network using an optically controllable antigen receptor. bioRxiv, 2020
  10. Optogenetic Control Reveals Differential Promoter Interpretation of Transcription Factor Nuclear Translocation Dynamics. Cell Syst, 2020
  11. Optogenetic Tuning of Protein-protein Binding in Bilayers Using LOVTRAP. Bio Protoc, 2020
  12. LITESEC-T3SS - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution. Nat Commun, 2020
  13. The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics. bioRxiv, 2020
  14. Actin waves transport RanGTP to the neurite tip to regulate non-centrosomal microtubules in neurons. J Cell Sci, 2020
  15. Optogenetics: Rho GTPases Activated by Light in Living Macrophages. Methods Mol Biol, 2020
  16. A Computational Protocol for Regulating Protein Binding Reactions with a Light-Sensitive Protein Dimer. J Mol Biol, 2019
  17. Optogenetic Control of Microtubule Dynamics. Methods Mol Biol, 2019
  18. Optogenetic control of cofilin and αTAT in living cells using Z-lock. Nat Chem Biol, 2019
  19. Using Tools from Optogenetics to Create Light-Responsive Biomaterials: LOVTRAP-PEG Hydrogels for Dynamic Peptide Immobilization. Ann Biomed Eng, 2019
  20. ESCRT-mediated phagophore sealing during mitophagy. Autophagy, 2019
  21. Optogenetic downregulation of protein levels with an ultrasensitive switch. ACS Synth Biol, 2019
  22. Light-based tuning of ligand half-life supports kinetic proofreading model of T cell signaling. Elife, 2019
  23. Luciferase-LOV BRET enables versatile and specific transcriptional readout of cellular protein-protein interactions. Elife, 2019
  24. Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET. Nat Chem Biol, 2018
  25. Rewiring Calcium Signaling for Precise Transcriptional Reprogramming. ACS Synth Biol, 2018
  26. Light-dependent cytoplasmic recruitment enhances the dynamic range of a nuclear import photoswitch. Chembiochem, 2018
  27. Local control of intracellular microtubule dynamics by EB1 photodissociation. Nat Cell Biol, 2018
  28. Discovery of long-range inhibitory signaling to ensure single axon formation. Nat Commun, 2017
  29. LOVTRAP: A Versatile Method to Control Protein Function with Light. Curr Protoc Cell Biol, 2016
  30. LOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat Methods, 2016
  31. Quantifying persistence in the T-cell signaling network using an optically controllable antigen receptor. Mol Syst Biol, 2021