AsLOV2

Photoreceptor AsLOV2
Binding partner /
Cofactor FMN
Source organism Avena sativa
Mode of action intramolecular conformational change
Excitation wavelength 450 nm
Reversion wavelength dark
Excitation time seconds
Reversion time tens of seconds
Crystal structure of AsLOV2, ground state. (PDB ID: 2V1A)

AsLOV2-derived switches:

Related original publications

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  1. Optogenetic manipulation of YAP cellular localisation and function. bioRxiv, 2021
  2. Structural Determinants for Light-Dependent Membrane Binding of a Photoswitchable Polybasic Domain. ACS Synth Biol, 2021
  3. A single-chain and fast-responding light-inducible Cre recombinase as a novel optogenetic switch. Elife, 2021
  4. Blue Light‐Operated CRISPR/Cas13b‐Mediated mRNA Knockdown (Lockdown). Adv Biol, 2021
  5. Optogenetic Control of Myocardin‐Related Transcription Factor A Subcellular Localization and Transcriptional Activity Steers Membrane Blebbing and Invasive Cancer Cell Motility. Adv Biol, 2021
  6. High levels of Dorsal transcription factor downregulate, not promote, snail expression by regulating enhancer action. bioRxiv, 2021
  7. Design of smart antibody mimetics with photosensitive switches. bioRxiv, 2020
  8. Livecell reporters reveal bidirectional acceleration of nucleocytoplasmic transport by O-GlcNAc modification of the nuclear pore complex. bioRxiv, 2020
  9. An optogenetic switch for the Set2 methyltransferase provides evidence for transcription-dependent and -independent dynamics of H3K36 methylation. Genome Res, 2020
  10. Optogenetic activation of heterotrimeric G-proteins by LOV2GIVe, a rationally engineered modular protein. Elife, 2020
  11. Optogenetic Control Reveals Differential Promoter Interpretation of Transcription Factor Nuclear Translocation Dynamics. Cell Syst, 2020
  12. Combination of photoactivation with lattice light-sheet imaging reveals untemplated, lamellar ruffle generation by PA-Rac1. bioRxiv, 2020
  13. Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold. Nat Commun, 2020
  14. Optogenetic control of protein binding using light-switchable nanobodies. Nat Commun, 2020
  15. Photo-SNAP-tag, a Light-Regulated Chemical Labeling System. ACS Chem Biol, 2020
  16. Optogenetic Downregulation of Protein Levels to Control Programmed Cell Death in Mammalian Cells with a Dual Blue-Light Switch. Methods Mol Biol, 2020
  17. Optogenetic Control of Nucleocytoplasmic Protein Transport. Methods Mol Biol, 2020
  18. Light-Inducible CRISPR Labeling. Methods Mol Biol, 2020
  19. Long-Range Optogenetic Control of Axon Guidance Overcomes Developmental Boundaries and Defects. Dev Cell, 2020
  20. A monogenic and fast-responding Light-Inducible Cre recombinase as a novel optogenetic switch. bioRxiv, 2020
  21. Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron. Genes Dev, 2020
  22. Combining optogenetics with sensitive FRET imaging to monitor local microtubule manipulations. Sci Rep, 2020
  23. The histone H4 basic patch regulates SAGA-mediated H2B deubiquitylation and histone acetylation. J Biol Chem, 2020
  24. An optogenetic switch for the Set2 methyltransferase provides evidence for rapid transcription-dependent and independent dynamics of H3K36 methylation. bioRxiv, 2020
  25. Optogenetic Control for Investigating Subcellular Localization of Fyn Kinase Activity in Single Live Cells. J Mol Biol, 2020
  26. Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling. Nat Commun, 2020
  27. Engineering light-controllable CAR T cells for cancer immunotherapy. Sci Adv, 2020
  28. Optogenetic Rac1 engineered from membrane lipid-binding RGS-LOV for inducible lamellipodia formation. Photochem Photobiol Sci, 2020
  29. Minimally disruptive optical control of protein tyrosine phosphatase 1B. Nat Commun, 2020
  30. Optogenetic regulation of endogenous proteins. Nat Commun, 2020
  31. Golgi-associated microtubules are fast cargo tracks and required for persistent cell migration. EMBO Rep, 2020
  32. Optogenetic control of spine-head JNK reveals a role in dendritic spine regression. eNeuro, 2020
  33. Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms. Nat Cell Biol, 2019
  34. Directed evolution improves the catalytic efficiency of TEV protease. Nat Methods, 2019
  35. Optogenetic control of Neisseria meningitidis Cas9 genome editing using an engineered, light-switchable anti-CRISPR protein. bioRxiv, 2019
  36. Visualization of a blue light transmission area in living animals using light-induced nuclear translocation of fluorescent proteins. Biochem Biophys Res Commun, 2019
  37. Locally Activating TrkB Receptor Generates Actin Waves and Specifies Axonal Fate. Cell Chem Biol, 2019
  38. Chemokine Receptor CCR7 Triggers an Endomembrane Signaling Complex for Spatial Rac Activation. Cell Rep, 2019
  39. Repurposing protein degradation for optogenetic modulation of protein activities. ACS Synth Biol, 2019
  40. Optogenetic Repressors of Gene Expression in Yeasts Using Light-Controlled Nuclear Localization. CMBE, 2019
  41. FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics. Nat Methods, 2019
  42. Coordination of protrusion dynamics within and between collectively migrating border cells by myosin II. Mol Biol Cell, 2019
  43. Reversible photocontrol of oxidase activity by inserting a photosensitive domain into the oxidase. BIOB, 2019
  44. VIEW-MOD: a versatile illumination engine with a modular optical design for fluorescence microscopy. Opt Express, 2019
  45. Optical control of transcription - genetically encoded photoswitchable variants of T7 RNA polymerase. Chembiochem, 2019
  46. Engineering Optogenetic Control of Endogenous p53 Protein Levels. Appl Sci, 2019
  47. Engineering proteins for allosteric control by light or ligands. Nat Protoc, 2019
  48. Optogenetic downregulation of protein levels with an ultrasensitive switch. ACS Synth Biol, 2019
  49. Optogenetic manipulation of stomatal kinetics improves carbon assimilation, water use, and growth. Science, 2019
  50. A yeast system for discovering optogenetic inhibitors of eukaryotic translation initiation. ACS Synth Biol, 2019
  51. Optogenetic control of cell morphogenesis on protein micropatterns. bioRxiv, 2019
  52. Optogenetic Navigation of Routes Leading to Protein Amyloidogenesis in Bacteria. J Mol Biol, 2019
  53. Photo‐ECM: A Blue Light Photoswitchable Synthetic Extracellular Matrix Protein for Reversible Control over Cell–Matrix Adhesion. Adv Biosyst, 2019
  54. Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing. Nat Commun, 2019
  55. Engineering Improved Photoswitches for the Control of Nucleocytoplasmic Distribution. ACS Synth Biol, 2018
  56. Integrating chemical and mechanical signals through dynamic coupling between cellular protrusions and pulsed ERK activation. Nat Commun, 2018
  57. Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. Nat Methods, 2018
  58. A light-gated potassium channel for sustained neuronal inhibition. Nat Methods, 2018
  59. Optogenetic manipulation of intracellular calcium by BACCS promotes differentiation of MC3T3-E1 cells. Biochem Biophys Res Commun, 2018
  60. Cyclic Stiffness Modulation of Cell‐Laden Protein–Polymer Hydrogels in Response to User‐Specified Stimuli Including Light. Adv Biosyst, 2018
  61. Dual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells. Sci Rep, 2018
  62. Fam49/CYRI interacts with Rac1 and locally suppresses protrusions. Nat Cell Biol, 2018
  63. Discovering selective binders for photoswitchable proteins using phage display. ACS Synth Biol, 2018
  64. Near-infrared light-controlled systems for gene transcription regulation, protein targeting and spectral multiplexing. Nat Protoc, 2018
  65. A Rac1-FMNL2 signaling module affects cell-cell contact formation independent of Cdc42 and membrane protrusions. PLoS ONE, 2018
  66. Rewiring Calcium Signaling for Precise Transcriptional Reprogramming. ACS Synth Biol, 2018
  67. Near-infrared light-controlled gene expression and protein targeting in neurons and non-neuronal cells. Chembiochem, 2018
  68. Time-gated detection of protein-protein interactions with transcriptional readout. Elife, 2017
  69. Engineering a light-activated caspase-3 for precise ablation of neurons in vivo. Proc Natl Acad Sci USA, 2017
  70. An engineered photoswitchable mammalian pyruvate kinase. FEBS J, 2017
  71. A calcium- and light-gated switch to induce gene expression in activated neurons. Nat Biotechnol, 2017
  72. A light- and calcium-gated transcription factor for imaging and manipulating activated neurons. Nat Biotechnol, 2017
  73. Rac1 switching at the right time and location is essential for Fcγ receptor-mediated phagosome formation. J Cell Sci, 2017
  74. Optical control of membrane tethering and interorganellar communication at nanoscales. Chem Sci, 2017
  75. A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat Commun, 2017
  76. Light-induced protein degradation in human-derived cells. Biochem Biophys Res Commun, 2017
  77. Near-infrared optogenetic pair for protein regulation and spectral multiplexing. Nat Chem Biol, 2017
  78. Kinetics of Endogenous CaMKII Required for Synaptic Plasticity Revealed by Optogenetic Kinase Inhibitor. Neuron, 2017
  79. Investigations of human myosin VI targeting using optogenetically controlled cargo loading. Proc Natl Acad Sci USA, 2017
  80. Engineering extrinsic disorder to control protein activity in living cells. Science, 2016
  81. Optogenetic Control of Nuclear Protein Import in Living Cells Using Light-Inducible Nuclear Localization Signals (LINuS). Curr Protoc Chem Biol, 2016
  82. Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol, 2016
  83. Post-translational control of protein function with light using a LOV-intein fusion protein. Mol Biosyst, 2016
  84. Optogenetic control of nuclear protein export. Nat Commun, 2016
  85. Regulation of neural gene transcription by optogenetic inhibition of the RE1-silencing transcription factor. Proc Natl Acad Sci USA, 2015
  86. Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation. Elife, 2015
  87. Photocontrolled Exposure of Pro-apoptotic Peptide Sequences in LOV Proteins Modulates Bcl-2 Family Interactions. Chembiochem, 2015
  88. Optical Control of Peroxisomal Trafficking. ACS Synth Biol, 2015
  89. Optogenetic control of endogenous Ca(2+) channels in vivo. Nat Biotechnol, 2015
  90. Labelling and optical erasure of synaptic memory traces in the motor cortex. Nature, 2015
  91. An Engineered Split Intein for Photoactivated Protein Trans-Splicing. PLoS ONE, 2015
  92. Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS. Nat Commun, 2015
  93. Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation. PLoS ONE, 2015
  94. Junctional actin assembly is mediated by Formin-like 2 downstream of Rac1. J Cell Biol, 2015
  95. Optogenetics. Engineering of a light-gated potassium channel. Science, 2015
  96. An optogenetic upgrade for the Tet-OFF system. Biotechnol Bioeng, 2015
  97. Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins. Proc Natl Acad Sci USA, 2014
  98. Structural details of light activation of the LOV2-based photoswitch PA-Rac1. ACS Chem Biol, 2014
  99. Remote control of myosin and kinesin motors using light-activated gearshifting. Nat Nanotechnol, 2014
  100. Engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells. Nat Commun, 2014
  101. Manipulation of endogenous kinase activity in living cells using photoswitchable inhibitory peptides. ACS Synth Biol, 2014
  102. Rac1-dependent lamellipodial motility in prostate cancer PC-3 cells revealed by optogenetic control of Rac1 activity. PLoS ONE, 2014
  103. Optical control of the Ca2+ concentration in a live specimen with a genetically encoded Ca2+-releasing molecular tool. ACS Chem Biol, 2014
  104. A fully genetically encoded protein architecture for optical control of peptide ligand concentration. Nat Commun, 2014
  105. General method for regulating protein stability with light. ACS Chem Biol, 2013
  106. RasGRF2 Rac-GEF activity couples NMDA receptor calcium flux to enhanced synaptic transmission. Proc Natl Acad Sci USA, 2013
  107. An optogenetic tool for the activation of endogenous diaphanous-related formins induces thickening of stress fibers without an increase in contractility. Cytoskeleton (Hoboken), 2013
  108. Nuclear actin network assembly by formins regulates the SRF coactivator MAL. Science, 2013
  109. Light activated cell migration in synthetic extracellular matrices. Biomaterials, 2012
  110. Controlling the DNA cleavage activity of light-inducible chimeric endonucleases by bidirectional photoactivation. Bioconjug Chem, 2012
  111. Designing photoswitchable peptides using the AsLOV2 domain. Chem Biol, 2012
  112. Engineering a photoactivated caspase-7 for rapid induction of apoptosis. ACS Synth Biol, 2011
  113. A synthetic photoactivated protein to generate local or global Ca(2+) signals. Chem Biol, 2011
  114. Rationally improving LOV domain-based photoswitches. Nat Methods, 2010
  115. Light-mediated activation reveals a key role for Rac in collective guidance of cell movement in vivo. Nat Cell Biol, 2010
  116. A genetically encoded photoactivatable Rac controls the motility of living cells. Nature, 2009
  117. Surface sites for engineering allosteric control in proteins. Science, 2008
  118. Light-activated DNA binding in a designed allosteric protein. Proc Natl Acad Sci USA, 2008
  119. Rac1 is essential in cocaine-induced structural plasticity of nucleus accumbens neurons. Nat Neurosci, 2012
  120. LOV2-Controlled Photoactivation of Protein Trans-Splicing. Methods Mol Biol, 2017