AsLOV2

Photoreceptor AsLOV2
Binding partner /
Cofactor FMN
Source organism Avena sativa
Mode of action intramolecular conformational change
Excitation wavelength 450 nm
Reversion wavelength dark
Excitation time seconds
Reversion time tens of seconds
Crystal structure of AsLOV2, ground state. (PDB ID: 2V1A)

AsLOV2-derived switches:

Related original publications

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  1. Combining optogenetics with sensitive FRET imaging to monitor local microtubule manipulations. Sci Rep, 2020
  2. The histone H4 basic patch regulates SAGA-mediated H2B deubiquitylation and histone acetylation. J Biol Chem, 2020
  3. An optogenetic switch for the Set2 methyltransferase provides evidence for rapid transcription-dependent and independent dynamics of H3K36 methylation. bioRxiv, 2020
  4. Optogenetic Control for Investigating Subcellular Localization of Fyn Kinase Activity in Single Live Cells. J Mol Biol, 2020
  5. Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling. Nat Commun, 2020
  6. Engineering light-controllable CAR T cells for cancer immunotherapy. Sci Adv, 2020
  7. Optogenetic Rac1 engineered from membrane lipid-binding RGS-LOV for inducible lamellipodia formation. Photochem Photobiol Sci, 2020
  8. Minimally disruptive optical control of protein tyrosine phosphatase 1B. Nat Commun, 2020
  9. Optogenetic regulation of endogenous proteins. Nat Commun, 2020
  10. Golgi-associated microtubules are fast cargo tracks and required for persistent cell migration. EMBO Rep, 2020
  11. Optogenetic control of spine-head JNK reveals a role in dendritic spine regression. eNeuro, 2020
  12. Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms. Nat Cell Biol, 2019
  13. Optogenetic control of Neisseria meningitidis Cas9 genome editing using an engineered, light-switchable anti-CRISPR protein. bioRxiv, 2019
  14. Visualization of a blue light transmission area in living animals using light-induced nuclear translocation of fluorescent proteins. Biochem Biophys Res Commun, 2019
  15. Locally Activating TrkB Receptor Generates Actin Waves and Specifies Axonal Fate. Cell Chem Biol, 2019
  16. Chemokine Receptor CCR7 Triggers an Endomembrane Signaling Complex for Spatial Rac Activation. Cell Rep, 2019
  17. Repurposing protein degradation for optogenetic modulation of protein activities. ACS Synth Biol, 2019
  18. Optogenetic Repressors of Gene Expression in Yeasts Using Light-Controlled Nuclear Localization. CMBE, 2019
  19. FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics. Nat Methods, 2019
  20. Optogenetic control of protein binding using light-switchable nanobodies. bioRxiv, 2019
  21. Coordination of protrusion dynamics within and between collectively migrating border cells by myosin II. Mol Biol Cell, 2019
  22. Reversible photocontrol of oxidase activity by inserting a photosensitive domain into the oxidase. BIOB, 2019
  23. VIEW-MOD: a versatile illumination engine with a modular optical design for fluorescence microscopy. Opt Express, 2019
  24. Optical control of transcription - genetically encoded photoswitchable variants of T7 RNA polymerase. Chembiochem, 2019
  25. Engineering Optogenetic Control of Endogenous p53 Protein Levels. Appl Sci, 2019
  26. Engineering proteins for allosteric control by light or ligands. Nat Protoc, 2019
  27. Optogenetic downregulation of protein levels with an ultrasensitive switch. ACS Synth Biol, 2019
  28. Optogenetic manipulation of stomatal kinetics improves carbon assimilation, water use, and growth. Science, 2019
  29. A yeast system for discovering optogenetic inhibitors of eukaryotic translation initiation. ACS Synth Biol, 2019
  30. Optogenetic control of cell morphogenesis on protein micropatterns. bioRxiv, 2019
  31. Optogenetic Navigation of Routes Leading to Protein Amyloidogenesis in Bacteria. J Mol Biol, 2019
  32. Photo‐ECM: A Blue Light Photoswitchable Synthetic Extracellular Matrix Protein for Reversible Control over Cell–Matrix Adhesion. Adv Biosyst, 2019
  33. Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing. Nat Commun, 2019
  34. Engineering Improved Photoswitches for the Control of Nucleocytoplasmic Distribution. ACS Synth Biol, 2018
  35. Integrating chemical and mechanical signals through dynamic coupling between cellular protrusions and pulsed ERK activation. Nat Commun, 2018
  36. Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. Nat Methods, 2018
  37. A light-gated potassium channel for sustained neuronal inhibition. Nat Methods, 2018
  38. Optogenetic manipulation of intracellular calcium by BACCS promotes differentiation of MC3T3-E1 cells. Biochem Biophys Res Commun, 2018
  39. Cyclic Stiffness Modulation of Cell‐Laden Protein–Polymer Hydrogels in Response to User‐Specified Stimuli Including Light. Adv Biosyst, 2018
  40. Dual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells. Sci Rep, 2018
  41. Fam49/CYRI interacts with Rac1 and locally suppresses protrusions. Nat Cell Biol, 2018
  42. Discovering selective binders for photoswitchable proteins using phage display. ACS Synth Biol, 2018
  43. Near-infrared light-controlled systems for gene transcription regulation, protein targeting and spectral multiplexing. Nat Protoc, 2018
  44. A Rac1-FMNL2 signaling module affects cell-cell contact formation independent of Cdc42 and membrane protrusions. PLoS ONE, 2018
  45. Rewiring Calcium Signaling for Precise Transcriptional Reprogramming. ACS Synth Biol, 2018
  46. Near-infrared light-controlled gene expression and protein targeting in neurons and non-neuronal cells. Chembiochem, 2018
  47. Time-gated detection of protein-protein interactions with transcriptional readout. Elife, 2017
  48. Engineering a light-activated caspase-3 for precise ablation of neurons in vivo. Proc Natl Acad Sci USA, 2017
  49. An engineered photoswitchable mammalian pyruvate kinase. FEBS J, 2017
  50. A calcium- and light-gated switch to induce gene expression in activated neurons. Nat Biotechnol, 2017
  51. A light- and calcium-gated transcription factor for imaging and manipulating activated neurons. Nat Biotechnol, 2017
  52. Rac1 switching at the right time and location is essential for Fcγ receptor-mediated phagosome formation. J Cell Sci, 2017
  53. Optical control of membrane tethering and interorganellar communication at nanoscales. Chem Sci, 2017
  54. A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat Commun, 2017
  55. Light-induced protein degradation in human-derived cells. Biochem Biophys Res Commun, 2017
  56. Near-infrared optogenetic pair for protein regulation and spectral multiplexing. Nat Chem Biol, 2017
  57. Kinetics of Endogenous CaMKII Required for Synaptic Plasticity Revealed by Optogenetic Kinase Inhibitor. Neuron, 2017
  58. Investigations of human myosin VI targeting using optogenetically controlled cargo loading. Proc Natl Acad Sci USA, 2017
  59. Engineering extrinsic disorder to control protein activity in living cells. Science, 2016
  60. Optogenetic Control of Nuclear Protein Import in Living Cells Using Light-Inducible Nuclear Localization Signals (LINuS). Curr Protoc Chem Biol, 2016
  61. Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol, 2016
  62. Post-translational control of protein function with light using a LOV-intein fusion protein. Mol Biosyst, 2016
  63. Optogenetic control of nuclear protein export. Nat Commun, 2016
  64. Regulation of neural gene transcription by optogenetic inhibition of the RE1-silencing transcription factor. Proc Natl Acad Sci USA, 2015
  65. Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation. Elife, 2015
  66. Photocontrolled Exposure of Pro-apoptotic Peptide Sequences in LOV Proteins Modulates Bcl-2 Family Interactions. Chembiochem, 2015
  67. Optical Control of Peroxisomal Trafficking. ACS Synth Biol, 2015
  68. Optogenetic control of endogenous Ca(2+) channels in vivo. Nat Biotechnol, 2015
  69. An Engineered Split Intein for Photoactivated Protein Trans-Splicing. PLoS ONE, 2015
  70. Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS. Nat Commun, 2015
  71. Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation. PLoS ONE, 2015
  72. Junctional actin assembly is mediated by Formin-like 2 downstream of Rac1. J Cell Biol, 2015
  73. Optogenetics. Engineering of a light-gated potassium channel. Science, 2015
  74. An optogenetic upgrade for the Tet-OFF system. Biotechnol Bioeng, 2015
  75. Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins. Proc Natl Acad Sci USA, 2014
  76. Structural details of light activation of the LOV2-based photoswitch PA-Rac1. ACS Chem Biol, 2014
  77. Remote control of myosin and kinesin motors using light-activated gearshifting. Nat Nanotechnol, 2014
  78. Engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells. Nat Commun, 2014
  79. Manipulation of endogenous kinase activity in living cells using photoswitchable inhibitory peptides. ACS Synth Biol, 2014
  80. Rac1-dependent lamellipodial motility in prostate cancer PC-3 cells revealed by optogenetic control of Rac1 activity. PLoS ONE, 2014
  81. Optical control of the Ca2+ concentration in a live specimen with a genetically encoded Ca2+-releasing molecular tool. ACS Chem Biol, 2014
  82. A fully genetically encoded protein architecture for optical control of peptide ligand concentration. Nat Commun, 2014
  83. General method for regulating protein stability with light. ACS Chem Biol, 2013
  84. RasGRF2 Rac-GEF activity couples NMDA receptor calcium flux to enhanced synaptic transmission. Proc Natl Acad Sci USA, 2013
  85. An optogenetic tool for the activation of endogenous diaphanous-related formins induces thickening of stress fibers without an increase in contractility. Cytoskeleton (Hoboken), 2013
  86. Nuclear actin network assembly by formins regulates the SRF coactivator MAL. Science, 2013
  87. Light activated cell migration in synthetic extracellular matrices. Biomaterials, 2012
  88. Controlling the DNA cleavage activity of light-inducible chimeric endonucleases by bidirectional photoactivation. Bioconjug Chem, 2012
  89. Designing photoswitchable peptides using the AsLOV2 domain. Chem Biol, 2012
  90. Engineering a photoactivated caspase-7 for rapid induction of apoptosis. ACS Synth Biol, 2011
  91. A synthetic photoactivated protein to generate local or global Ca(2+) signals. Chem Biol, 2011
  92. Rationally improving LOV domain-based photoswitches. Nat Methods, 2010
  93. Light-mediated activation reveals a key role for Rac in collective guidance of cell movement in vivo. Nat Cell Biol, 2010
  94. A genetically encoded photoactivatable Rac controls the motility of living cells. Nature, 2009
  95. Surface sites for engineering allosteric control in proteins. Science, 2008
  96. Light-activated DNA binding in a designed allosteric protein. Proc Natl Acad Sci USA, 2008
  97. Rac1 is essential in cocaine-induced structural plasticity of nucleus accumbens neurons. Nat Neurosci, 2012
  98. LOV2-Controlled Photoactivation of Protein Trans-Splicing. Methods Mol Biol, 2017