AsLOV2

Photoreceptor AsLOV2
Binding partner /
Cofactor FMN
Source organism Avena sativa
Mode of action intramolecular conformational change
Excitation wavelength 450 nm
Reversion wavelength dark
Excitation time seconds
Reversion time tens of seconds
Crystal structure of AsLOV2, ground state. (PDB ID: 2V1A)

AsLOV2-derived switches:

Related original publications

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  1. Integrating chemical and mechanical signals through dynamic coupling between cellular protrusions and pulsed ERK activation. Nat Commun, 2018
  2. Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. Nat Methods, 2018
  3. Cyclic Stiffness Modulation of Cell‐Laden Protein–Polymer Hydrogels in Response to User‐Specified Stimuli Including Light. Adv Biosyst, 2018
  4. Dual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells. Sci Rep, 2018
  5. Fam49/CYRI interacts with Rac1 and locally suppresses protrusions. Nat Cell Biol, 2018
  6. Discovering selective binders for photoswitchable proteins using phage display. ACS Synth Biol, 2018
  7. Near-infrared light-controlled systems for gene transcription regulation, protein targeting and spectral multiplexing. Nat Protoc, 2018
  8. A Rac1-FMNL2 signaling module affects cell-cell contact formation independent of Cdc42 and membrane protrusions. PLoS ONE, 2018
  9. Rewiring Calcium Signaling for Precise Transcriptional Reprogramming. ACS Synth Biol, 2018
  10. Near-infrared light-controlled gene expression and protein targeting in neurons and non-neuronal cells. Chembiochem, 2018
  11. Engineering a light-activated caspase-3 for precise ablation of neurons in vivo. Proc Natl Acad Sci USA, 2017
  12. An engineered photoswitchable mammalian pyruvate kinase. FEBS J, 2017
  13. A calcium- and light-gated switch to induce gene expression in activated neurons. Nat Biotechnol, 2017
  14. A light- and calcium-gated transcription factor for imaging and manipulating activated neurons. Nat Biotechnol, 2017
  15. Rac1 switching at the right time and location is essential for Fcγ receptor-mediated phagosome formation. J Cell Sci, 2017
  16. Optical control of membrane tethering and interorganellar communication at nanoscales. Chem Sci, 2017
  17. A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat Commun, 2017
  18. Light-induced protein degradation in human-derived cells. Biochem Biophys Res Commun, 2017
  19. Near-infrared optogenetic pair for protein regulation and spectral multiplexing. Nat Chem Biol, 2017
  20. Kinetics of Endogenous CaMKII Required for Synaptic Plasticity Revealed by Optogenetic Kinase Inhibitor. Neuron, 2017
  21. Investigations of human myosin VI targeting using optogenetically controlled cargo loading. Proc Natl Acad Sci USA, 2017
  22. Engineering extrinsic disorder to control protein activity in living cells. Science, 2016
  23. Optogenetic Control of Nuclear Protein Import in Living Cells Using Light-Inducible Nuclear Localization Signals (LINuS). Curr Protoc Chem Biol, 2016
  24. Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol, 2016
  25. Optogenetic control of nuclear protein export. Nat Commun, 2016
  26. Regulation of neural gene transcription by optogenetic inhibition of the RE1-silencing transcription factor. Proc Natl Acad Sci USA, 2015
  27. Photocontrolled Exposure of Pro-apoptotic Peptide Sequences in LOV Proteins Modulates Bcl-2 Family Interactions. Chembiochem, 2015
  28. Optical Control of Peroxisomal Trafficking. ACS Synth Biol, 2015
  29. Optogenetic control of endogenous Ca(2+) channels in vivo. Nat Biotechnol, 2015
  30. An Engineered Split Intein for Photoactivated Protein Trans-Splicing. PLoS ONE, 2015
  31. Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation. PLoS ONE, 2015
  32. Junctional actin assembly is mediated by Formin-like 2 downstream of Rac1. J Cell Biol, 2015
  33. Optogenetics. Engineering of a light-gated potassium channel. Science, 2015
  34. An optogenetic upgrade for the Tet-OFF system. Biotechnol Bioeng, 2015
  35. Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins. Proc Natl Acad Sci USA, 2014
  36. Structural details of light activation of the LOV2-based photoswitch PA-Rac1. ACS Chem Biol, 2014
  37. Engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells. Nat Commun, 2014
  38. Manipulation of endogenous kinase activity in living cells using photoswitchable inhibitory peptides. ACS Synth Biol, 2014
  39. Rac1-dependent lamellipodial motility in prostate cancer PC-3 cells revealed by optogenetic control of Rac1 activity. PLoS ONE, 2014
  40. Optical control of the Ca2+ concentration in a live specimen with a genetically encoded Ca2+-releasing molecular tool. ACS Chem Biol, 2014
  41. A fully genetically encoded protein architecture for optical control of peptide ligand concentration. Nat Commun, 2014
  42. General method for regulating protein stability with light. ACS Chem Biol, 2013
  43. RasGRF2 Rac-GEF activity couples NMDA receptor calcium flux to enhanced synaptic transmission. Proc Natl Acad Sci USA, 2013
  44. An optogenetic tool for the activation of endogenous diaphanous-related formins induces thickening of stress fibers without an increase in contractility. Cytoskeleton (Hoboken), 2013
  45. Nuclear actin network assembly by formins regulates the SRF coactivator MAL. Science, 2013
  46. Light activated cell migration in synthetic extracellular matrices. Biomaterials, 2012
  47. Controlling the DNA cleavage activity of light-inducible chimeric endonucleases by bidirectional photoactivation. Bioconjug Chem, 2012
  48. Designing photoswitchable peptides using the AsLOV2 domain. Chem Biol, 2012
  49. Engineering a photoactivated caspase-7 for rapid induction of apoptosis. ACS Synth Biol, 2011
  50. A synthetic photoactivated protein to generate local or global Ca(2+) signals. Chem Biol, 2011
  51. Rationally improving LOV domain-based photoswitches. Nat Methods, 2010
  52. A genetically encoded photoactivatable Rac controls the motility of living cells. Nature, 2009
  53. Surface sites for engineering allosteric control in proteins. Science, 2008
  54. Light-activated DNA binding in a designed allosteric protein. Proc Natl Acad Sci USA, 2008
  55. Rac1 is essential in cocaine-induced structural plasticity of nucleus accumbens neurons. Nat Neurosci, 2012