AsLOV2

Photoreceptor AsLOV2
Binding partner /
Cofactor FMN
Source organism Avena sativa
Mode of action intramolecular conformational change
Excitation wavelength 450 nm
Reversion wavelength dark
Excitation time seconds
Reversion time tens of seconds
Crystal structure of AsLOV2, ground state. (PDB ID: 2V1A)

AsLOV2-derived switches:

Related original publications

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  1. Locally Activating TrkB Receptor Generates Actin Waves and Specifies Axonal Fate. Cell Chem Biol, 2019
  2. Chemokine Receptor CCR7 Triggers an Endomembrane Signaling Complex for Spatial Rac Activation. Cell Rep, 2019
  3. Repurposing protein degradation for optogenetic modulation of protein activities. ACS Synth Biol, 2019
  4. Optogenetic Repressors of Gene Expression in Yeasts Using Light-Controlled Nuclear Localization. CMBE, 2019
  5. FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics. Nat Methods, 2019
  6. Optogenetic control of protein binding using light-switchable nanobodies. bioRxiv, 2019
  7. Coordination of protrusion dynamics within and between collectively migrating border cells by myosin II. Mol Biol Cell, 2019
  8. Reversible photocontrol of oxidase activity by inserting a photosensitive domain into the oxidase. BIOB, 2019
  9. VIEW-MOD: a versatile illumination engine with a modular optical design for fluorescence microscopy. Opt Express, 2019
  10. Repurposing protein degradation for optogenetic modulation of protein activities. bioRxiv, 2019
  11. Optical control of transcription - genetically encoded photoswitchable variants of T7 RNA polymerase. Chembiochem, 2019
  12. Engineering Optogenetic Control of Endogenous p53 Protein Levels. Appl Sci, 2019
  13. Engineering proteins for allosteric control by light or ligands. Nat Protoc, 2019
  14. Optogenetic downregulation of protein levels with an ultrasensitive switch. ACS Synth Biol, 2019
  15. Optogenetic manipulation of stomatal kinetics improves carbon assimilation, water use, and growth. Science, 2019
  16. A yeast system for discovering optogenetic inhibitors of eukaryotic translation initiation. ACS Synth Biol, 2019
  17. Optogenetic control of cell morphogenesis on protein micropatterns. bioRxiv, 2019
  18. Optogenetic Navigation of Routes Leading to Protein Amyloidogenesis in Bacteria. J Mol Biol, 2019
  19. Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing. Nat Commun, 2019
  20. Engineering Improved Photoswitches for the Control of Nucleocytoplasmic Distribution. ACS Synth Biol, 2018
  21. Integrating chemical and mechanical signals through dynamic coupling between cellular protrusions and pulsed ERK activation. Nat Commun, 2018
  22. Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. Nat Methods, 2018
  23. A light-gated potassium channel for sustained neuronal inhibition. Nat Methods, 2018
  24. Cyclic Stiffness Modulation of Cell‐Laden Protein–Polymer Hydrogels in Response to User‐Specified Stimuli Including Light. Adv Biosyst, 2018
  25. Dual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells. Sci Rep, 2018
  26. Fam49/CYRI interacts with Rac1 and locally suppresses protrusions. Nat Cell Biol, 2018
  27. Discovering selective binders for photoswitchable proteins using phage display. ACS Synth Biol, 2018
  28. Near-infrared light-controlled systems for gene transcription regulation, protein targeting and spectral multiplexing. Nat Protoc, 2018
  29. A Rac1-FMNL2 signaling module affects cell-cell contact formation independent of Cdc42 and membrane protrusions. PLoS ONE, 2018
  30. Rewiring Calcium Signaling for Precise Transcriptional Reprogramming. ACS Synth Biol, 2018
  31. Near-infrared light-controlled gene expression and protein targeting in neurons and non-neuronal cells. Chembiochem, 2018
  32. Time-gated detection of protein-protein interactions with transcriptional readout. Elife, 2017
  33. Engineering a light-activated caspase-3 for precise ablation of neurons in vivo. Proc Natl Acad Sci USA, 2017
  34. An engineered photoswitchable mammalian pyruvate kinase. FEBS J, 2017
  35. A calcium- and light-gated switch to induce gene expression in activated neurons. Nat Biotechnol, 2017
  36. A light- and calcium-gated transcription factor for imaging and manipulating activated neurons. Nat Biotechnol, 2017
  37. Rac1 switching at the right time and location is essential for Fcγ receptor-mediated phagosome formation. J Cell Sci, 2017
  38. Optical control of membrane tethering and interorganellar communication at nanoscales. Chem Sci, 2017
  39. A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat Commun, 2017
  40. Light-induced protein degradation in human-derived cells. Biochem Biophys Res Commun, 2017
  41. Near-infrared optogenetic pair for protein regulation and spectral multiplexing. Nat Chem Biol, 2017
  42. Kinetics of Endogenous CaMKII Required for Synaptic Plasticity Revealed by Optogenetic Kinase Inhibitor. Neuron, 2017
  43. Investigations of human myosin VI targeting using optogenetically controlled cargo loading. Proc Natl Acad Sci USA, 2017
  44. Engineering extrinsic disorder to control protein activity in living cells. Science, 2016
  45. Optogenetic Control of Nuclear Protein Import in Living Cells Using Light-Inducible Nuclear Localization Signals (LINuS). Curr Protoc Chem Biol, 2016
  46. Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol, 2016
  47. Post-translational control of protein function with light using a LOV-intein fusion protein. Mol Biosyst, 2016
  48. Optogenetic control of nuclear protein export. Nat Commun, 2016
  49. Regulation of neural gene transcription by optogenetic inhibition of the RE1-silencing transcription factor. Proc Natl Acad Sci USA, 2015
  50. Photocontrolled Exposure of Pro-apoptotic Peptide Sequences in LOV Proteins Modulates Bcl-2 Family Interactions. Chembiochem, 2015
  51. Optical Control of Peroxisomal Trafficking. ACS Synth Biol, 2015
  52. Optogenetic control of endogenous Ca(2+) channels in vivo. Nat Biotechnol, 2015
  53. An Engineered Split Intein for Photoactivated Protein Trans-Splicing. PLoS ONE, 2015
  54. Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation. PLoS ONE, 2015
  55. Junctional actin assembly is mediated by Formin-like 2 downstream of Rac1. J Cell Biol, 2015
  56. Optogenetics. Engineering of a light-gated potassium channel. Science, 2015
  57. An optogenetic upgrade for the Tet-OFF system. Biotechnol Bioeng, 2015
  58. Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins. Proc Natl Acad Sci USA, 2014
  59. Structural details of light activation of the LOV2-based photoswitch PA-Rac1. ACS Chem Biol, 2014
  60. Remote control of myosin and kinesin motors using light-activated gearshifting. Nat Nanotechnol, 2014
  61. Engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells. Nat Commun, 2014
  62. Manipulation of endogenous kinase activity in living cells using photoswitchable inhibitory peptides. ACS Synth Biol, 2014
  63. Rac1-dependent lamellipodial motility in prostate cancer PC-3 cells revealed by optogenetic control of Rac1 activity. PLoS ONE, 2014
  64. Optical control of the Ca2+ concentration in a live specimen with a genetically encoded Ca2+-releasing molecular tool. ACS Chem Biol, 2014
  65. A fully genetically encoded protein architecture for optical control of peptide ligand concentration. Nat Commun, 2014
  66. General method for regulating protein stability with light. ACS Chem Biol, 2013
  67. RasGRF2 Rac-GEF activity couples NMDA receptor calcium flux to enhanced synaptic transmission. Proc Natl Acad Sci USA, 2013
  68. An optogenetic tool for the activation of endogenous diaphanous-related formins induces thickening of stress fibers without an increase in contractility. Cytoskeleton (Hoboken), 2013
  69. Nuclear actin network assembly by formins regulates the SRF coactivator MAL. Science, 2013
  70. Light activated cell migration in synthetic extracellular matrices. Biomaterials, 2012
  71. Controlling the DNA cleavage activity of light-inducible chimeric endonucleases by bidirectional photoactivation. Bioconjug Chem, 2012
  72. Designing photoswitchable peptides using the AsLOV2 domain. Chem Biol, 2012
  73. Engineering a photoactivated caspase-7 for rapid induction of apoptosis. ACS Synth Biol, 2011
  74. A synthetic photoactivated protein to generate local or global Ca(2+) signals. Chem Biol, 2011
  75. Rationally improving LOV domain-based photoswitches. Nat Methods, 2010
  76. Light-mediated activation reveals a key role for Rac in collective guidance of cell movement in vivo. Nat Cell Biol, 2010
  77. A genetically encoded photoactivatable Rac controls the motility of living cells. Nature, 2009
  78. Surface sites for engineering allosteric control in proteins. Science, 2008
  79. Light-activated DNA binding in a designed allosteric protein. Proc Natl Acad Sci USA, 2008
  80. Rac1 is essential in cocaine-induced structural plasticity of nucleus accumbens neurons. Nat Neurosci, 2012
  81. LOV2-Controlled Photoactivation of Protein Trans-Splicing. Methods Mol Biol, 2017