AsLOV2

Photoreceptor AsLOV2
Binding partner /
Cofactor FMN
Source organism Avena sativa
Mode of action intramolecular conformational change
Excitation wavelength 450 nm
Reversion wavelength dark
Excitation time seconds
Reversion time tens of seconds
Crystal structure of AsLOV2, ground state. (PDB ID: 2V1A)

AsLOV2-derived switches:

Related original publications

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  1. Desensitisation of Notch signalling through dynamic adaptation in the nucleus. EMBO J, 2021
  2. Mechanical worrying drives cell migration in crowded environments. bioRxiv, 2021
  3. Extremely rapid and reversible optogenetic perturbation of nuclear proteins in living embryos. Dev Cell, 2021
  4. Mechanosensitivity of nucleocytoplasmic transport. bioRxiv, 2021
  5. Circularly permuted AsLOV2 as an optogenetic module for engineering photoswitchable peptides. Chem Commun (Camb), 2021
  6. Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells. J Vis Exp, 2021
  7. O-GlcNAc modification of nuclear pore complexes accelerates bidirectional transport. J Cell Biol, 2021
  8. Engineering a Blue Light Inducible SpyTag System (BLISS). J Am Chem Soc, 2021
  9. Rab10-Positive Tubular Structures Represent a Novel Endocytic Pathway That Diverges From Canonical Macropinocytosis in RAW264 Macrophages. Front Immunol, 2021
  10. A photo-switchable yeast isocitrate dehydrogenase to control metabolic flux through the citric acid cycle. bioRxiv, 2021
  11. Light-dependent N-end rule-mediated disruption of protein function in Saccharomyces cerevisiae and Drosophila melanogaster. PLoS Genet, 2021
  12. Circularly permuted LOV2 as a modular photoswitch for optogenetic engineering. Nat Chem Biol, 2021
  13. Rac1 activation can generate untemplated, lamellar membrane ruffles. BMC Biol, 2021
  14. Optogenetic manipulation of YAP cellular localisation and function. bioRxiv, 2021
  15. Structural Determinants for Light-Dependent Membrane Binding of a Photoswitchable Polybasic Domain. ACS Synth Biol, 2021
  16. A single-chain and fast-responding light-inducible Cre recombinase as a novel optogenetic switch. Elife, 2021
  17. Blue Light‐Operated CRISPR/Cas13b‐Mediated mRNA Knockdown (Lockdown). Adv Biol, 2021
  18. Optogenetic Control of Myocardin‐Related Transcription Factor A Subcellular Localization and Transcriptional Activity Steers Membrane Blebbing and Invasive Cancer Cell Motility. Adv Biol, 2021
  19. Design of Smart Antibody Mimetics with Photosensitive Switches. Adv Biol (Weinh), 2021
  20. High levels of Dorsal transcription factor downregulate, not promote, snail expression by regulating enhancer action. bioRxiv, 2021
  21. Design of smart antibody mimetics with photosensitive switches. bioRxiv, 2020
  22. Livecell reporters reveal bidirectional acceleration of nucleocytoplasmic transport by O-GlcNAc modification of the nuclear pore complex. bioRxiv, 2020
  23. An optogenetic switch for the Set2 methyltransferase provides evidence for transcription-dependent and -independent dynamics of H3K36 methylation. Genome Res, 2020
  24. Optogenetic activation of heterotrimeric G-proteins by LOV2GIVe, a rationally engineered modular protein. Elife, 2020
  25. Optogenetic Control Reveals Differential Promoter Interpretation of Transcription Factor Nuclear Translocation Dynamics. Cell Syst, 2020
  26. Combination of photoactivation with lattice light-sheet imaging reveals untemplated, lamellar ruffle generation by PA-Rac1. bioRxiv, 2020
  27. Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold. Nat Commun, 2020
  28. Optogenetic control of protein binding using light-switchable nanobodies. Nat Commun, 2020
  29. Photo-SNAP-tag, a Light-Regulated Chemical Labeling System. ACS Chem Biol, 2020
  30. Optogenetic Downregulation of Protein Levels to Control Programmed Cell Death in Mammalian Cells with a Dual Blue-Light Switch. Methods Mol Biol, 2020
  31. Optogenetic Control of Nucleocytoplasmic Protein Transport. Methods Mol Biol, 2020
  32. Light-Inducible CRISPR Labeling. Methods Mol Biol, 2020
  33. Long-Range Optogenetic Control of Axon Guidance Overcomes Developmental Boundaries and Defects. Dev Cell, 2020
  34. A monogenic and fast-responding Light-Inducible Cre recombinase as a novel optogenetic switch. bioRxiv, 2020
  35. Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron. Genes Dev, 2020
  36. Combining optogenetics with sensitive FRET imaging to monitor local microtubule manipulations. Sci Rep, 2020
  37. The histone H4 basic patch regulates SAGA-mediated H2B deubiquitylation and histone acetylation. J Biol Chem, 2020
  38. Optogenetic Control for Investigating Subcellular Localization of Fyn Kinase Activity in Single Live Cells. J Mol Biol, 2020
  39. Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling. Nat Commun, 2020
  40. Engineering light-controllable CAR T cells for cancer immunotherapy. Sci Adv, 2020
  41. Optogenetic Rac1 engineered from membrane lipid-binding RGS-LOV for inducible lamellipodia formation. Photochem Photobiol Sci, 2020
  42. Minimally disruptive optical control of protein tyrosine phosphatase 1B. Nat Commun, 2020
  43. Optogenetic regulation of endogenous proteins. Nat Commun, 2020
  44. Golgi-associated microtubules are fast cargo tracks and required for persistent cell migration. EMBO Rep, 2020
  45. Optogenetic control of spine-head JNK reveals a role in dendritic spine regression. eNeuro, 2020
  46. Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms. Nat Cell Biol, 2019
  47. Directed evolution improves the catalytic efficiency of TEV protease. Nat Methods, 2019
  48. Optogenetic control of Neisseria meningitidis Cas9 genome editing using an engineered, light-switchable anti-CRISPR protein. bioRxiv, 2019
  49. Visualization of a blue light transmission area in living animals using light-induced nuclear translocation of fluorescent proteins. Biochem Biophys Res Commun, 2019
  50. Locally Activating TrkB Receptor Generates Actin Waves and Specifies Axonal Fate. Cell Chem Biol, 2019
  51. Chemokine Receptor CCR7 Triggers an Endomembrane Signaling Complex for Spatial Rac Activation. Cell Rep, 2019
  52. Repurposing protein degradation for optogenetic modulation of protein activities. ACS Synth Biol, 2019
  53. Optogenetic Repressors of Gene Expression in Yeasts Using Light-Controlled Nuclear Localization. CMBE, 2019
  54. FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics. Nat Methods, 2019
  55. Coordination of protrusion dynamics within and between collectively migrating border cells by myosin II. Mol Biol Cell, 2019
  56. Reversible photocontrol of oxidase activity by inserting a photosensitive domain into the oxidase. BIOB, 2019
  57. VIEW-MOD: a versatile illumination engine with a modular optical design for fluorescence microscopy. Opt Express, 2019
  58. Optical control of transcription - genetically encoded photoswitchable variants of T7 RNA polymerase. Chembiochem, 2019
  59. Engineering Optogenetic Control of Endogenous p53 Protein Levels. Appl Sci, 2019
  60. Engineering proteins for allosteric control by light or ligands. Nat Protoc, 2019
  61. Optogenetic downregulation of protein levels with an ultrasensitive switch. ACS Synth Biol, 2019
  62. Optogenetic manipulation of stomatal kinetics improves carbon assimilation, water use, and growth. Science, 2019
  63. A yeast system for discovering optogenetic inhibitors of eukaryotic translation initiation. ACS Synth Biol, 2019
  64. Optogenetic control of cell morphogenesis on protein micropatterns. bioRxiv, 2019
  65. Optogenetic Navigation of Routes Leading to Protein Amyloidogenesis in Bacteria. J Mol Biol, 2019
  66. Photo‐ECM: A Blue Light Photoswitchable Synthetic Extracellular Matrix Protein for Reversible Control over Cell–Matrix Adhesion. Adv Biosyst, 2019
  67. Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing. Nat Commun, 2019
  68. Engineering Improved Photoswitches for the Control of Nucleocytoplasmic Distribution. ACS Synth Biol, 2018
  69. Integrating chemical and mechanical signals through dynamic coupling between cellular protrusions and pulsed ERK activation. Nat Commun, 2018
  70. Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. Nat Methods, 2018
  71. A light-gated potassium channel for sustained neuronal inhibition. Nat Methods, 2018
  72. Optogenetic manipulation of intracellular calcium by BACCS promotes differentiation of MC3T3-E1 cells. Biochem Biophys Res Commun, 2018
  73. Cyclic Stiffness Modulation of Cell‐Laden Protein–Polymer Hydrogels in Response to User‐Specified Stimuli Including Light. Adv Biosyst, 2018
  74. Dual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells. Sci Rep, 2018
  75. Fam49/CYRI interacts with Rac1 and locally suppresses protrusions. Nat Cell Biol, 2018
  76. Discovering selective binders for photoswitchable proteins using phage display. ACS Synth Biol, 2018
  77. Near-infrared light-controlled systems for gene transcription regulation, protein targeting and spectral multiplexing. Nat Protoc, 2018
  78. A Rac1-FMNL2 signaling module affects cell-cell contact formation independent of Cdc42 and membrane protrusions. PLoS ONE, 2018
  79. Rewiring Calcium Signaling for Precise Transcriptional Reprogramming. ACS Synth Biol, 2018
  80. Near-infrared light-controlled gene expression and protein targeting in neurons and non-neuronal cells. Chembiochem, 2018
  81. Time-gated detection of protein-protein interactions with transcriptional readout. Elife, 2017
  82. Engineering a light-activated caspase-3 for precise ablation of neurons in vivo. Proc Natl Acad Sci USA, 2017
  83. An engineered photoswitchable mammalian pyruvate kinase. FEBS J, 2017
  84. A calcium- and light-gated switch to induce gene expression in activated neurons. Nat Biotechnol, 2017
  85. A light- and calcium-gated transcription factor for imaging and manipulating activated neurons. Nat Biotechnol, 2017
  86. Rac1 switching at the right time and location is essential for Fcγ receptor-mediated phagosome formation. J Cell Sci, 2017
  87. Optical control of membrane tethering and interorganellar communication at nanoscales. Chem Sci, 2017
  88. A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat Commun, 2017
  89. Light-induced protein degradation in human-derived cells. Biochem Biophys Res Commun, 2017
  90. Near-infrared optogenetic pair for protein regulation and spectral multiplexing. Nat Chem Biol, 2017
  91. Kinetics of Endogenous CaMKII Required for Synaptic Plasticity Revealed by Optogenetic Kinase Inhibitor. Neuron, 2017
  92. Investigations of human myosin VI targeting using optogenetically controlled cargo loading. Proc Natl Acad Sci USA, 2017
  93. Engineering extrinsic disorder to control protein activity in living cells. Science, 2016
  94. Optogenetic Control of Nuclear Protein Import in Living Cells Using Light-Inducible Nuclear Localization Signals (LINuS). Curr Protoc Chem Biol, 2016
  95. Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol, 2016
  96. Post-translational control of protein function with light using a LOV-intein fusion protein. Mol Biosyst, 2016
  97. Optogenetic control of nuclear protein export. Nat Commun, 2016
  98. Regulation of neural gene transcription by optogenetic inhibition of the RE1-silencing transcription factor. Proc Natl Acad Sci USA, 2015
  99. Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation. Elife, 2015
  100. Photocontrolled Exposure of Pro-apoptotic Peptide Sequences in LOV Proteins Modulates Bcl-2 Family Interactions. Chembiochem, 2015
  101. Optical Control of Peroxisomal Trafficking. ACS Synth Biol, 2015
  102. Optogenetic control of endogenous Ca(2+) channels in vivo. Nat Biotechnol, 2015
  103. Labelling and optical erasure of synaptic memory traces in the motor cortex. Nature, 2015
  104. An Engineered Split Intein for Photoactivated Protein Trans-Splicing. PLoS ONE, 2015
  105. Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS. Nat Commun, 2015
  106. Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation. PLoS ONE, 2015
  107. Junctional actin assembly is mediated by Formin-like 2 downstream of Rac1. J Cell Biol, 2015
  108. Optogenetics. Engineering of a light-gated potassium channel. Science, 2015
  109. An optogenetic upgrade for the Tet-OFF system. Biotechnol Bioeng, 2015
  110. Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins. Proc Natl Acad Sci USA, 2014
  111. Structural details of light activation of the LOV2-based photoswitch PA-Rac1. ACS Chem Biol, 2014
  112. Remote control of myosin and kinesin motors using light-activated gearshifting. Nat Nanotechnol, 2014
  113. Engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells. Nat Commun, 2014
  114. Manipulation of endogenous kinase activity in living cells using photoswitchable inhibitory peptides. ACS Synth Biol, 2014
  115. Rac1-dependent lamellipodial motility in prostate cancer PC-3 cells revealed by optogenetic control of Rac1 activity. PLoS ONE, 2014
  116. Optical control of the Ca2+ concentration in a live specimen with a genetically encoded Ca2+-releasing molecular tool. ACS Chem Biol, 2014
  117. A fully genetically encoded protein architecture for optical control of peptide ligand concentration. Nat Commun, 2014
  118. General method for regulating protein stability with light. ACS Chem Biol, 2013
  119. RasGRF2 Rac-GEF activity couples NMDA receptor calcium flux to enhanced synaptic transmission. Proc Natl Acad Sci USA, 2013
  120. An optogenetic tool for the activation of endogenous diaphanous-related formins induces thickening of stress fibers without an increase in contractility. Cytoskeleton (Hoboken), 2013
  121. Nuclear actin network assembly by formins regulates the SRF coactivator MAL. Science, 2013
  122. Light activated cell migration in synthetic extracellular matrices. Biomaterials, 2012
  123. Controlling the DNA cleavage activity of light-inducible chimeric endonucleases by bidirectional photoactivation. Bioconjug Chem, 2012
  124. Designing photoswitchable peptides using the AsLOV2 domain. Chem Biol, 2012
  125. Engineering a photoactivated caspase-7 for rapid induction of apoptosis. ACS Synth Biol, 2011
  126. A synthetic photoactivated protein to generate local or global Ca(2+) signals. Chem Biol, 2011
  127. Rationally improving LOV domain-based photoswitches. Nat Methods, 2010
  128. Light-mediated activation reveals a key role for Rac in collective guidance of cell movement in vivo. Nat Cell Biol, 2010
  129. A genetically encoded photoactivatable Rac controls the motility of living cells. Nature, 2009
  130. Surface sites for engineering allosteric control in proteins. Science, 2008
  131. Light-activated DNA binding in a designed allosteric protein. Proc Natl Acad Sci USA, 2008
  132. Rac1 is essential in cocaine-induced structural plasticity of nucleus accumbens neurons. Nat Neurosci, 2012
  133. LOV2-Controlled Photoactivation of Protein Trans-Splicing. Methods Mol Biol, 2017
  134. SPARK: A Transcriptional Assay for Recording Protein-Protein Interactions in a Defined Time Window. Curr Protoc, 2021