AsLOV2

Related original publications

1. Ultralow Background Membrane Editors for Spatiotemporal Control of Phosphatidic Acid Metabolism and Signaling ACS Cent Sci, 2024
2. Quantitative comparison of nuclear transport inhibition by SARS coronavirus ORF6 reveals the importance of oligomerization. Proc Natl Acad Sci U S A, 2024
3. Light inducible protein degradation in E. coli with the LOVdeg tag. Elife, 2024
4. Rapid characterization of anti-CRISPR proteins and optogenetically engineered variants using a versatile plasmid interference system. Nucleic Acids Res, 2023
5. Optogenetic control of YAP reveals a dynamic communication code for stem cell fate and proliferation. Nat Commun, 2023
6. Optogenetics for sensors: On-demand fluorescent labeling of histone epigenetics. Biochem Biophys Res Commun, 2023
7. Turn-On Protein Switches for Controlling Actin Binding in Cells. bioRxiv, 2023
8. Reversible photoregulation of cell-cell adhesions with opto-E-cadherin. Nat Commun, 2023
9. Photoactivatable base editors for spatiotemporally controlled genome editing in vivo. Biomaterials, 2023
10. Cell Cycle Control by Optogenetically Regulated Cell Cycle Inhibitor Protein p21. Biology (Basel), 2023
11. Optogenetic control of Cdc48 for dynamic metabolic engineering in yeast. Metab Eng, 2023
12. Light-switchable transcription factors obtained by direct screening in mammalian cells. Nat Commun, 2023
13. Engineered allostery in light-regulated LOV-Turbo enables precise spatiotemporal control of proximity labeling in living cells. Nat Methods, 2023
14. OptIC Notch reveals mechanism that regulates receptor interactions with CSL. Development, 2023
15. Optogenetic control of YAP can enhance the rate of wound healing. Cell Mol Biol Lett, 2023
16. Engineering of NEMO as calcium indicators with large dynamics and high sensitivity. Nat Methods, 2023
17. Requirements for mammalian promoters to decode transcription factor dynamics. Nucleic Acids Res, 2023
18. Allosteric inactivation of an engineered optogenetic GTPase. Proc Natl Acad Sci U S A, 2023
19. Light inducible protein degradation in E. coli with LOVtag. bioRxiv, 2023
20. An engineered N-acyltransferase-LOV2 domain fusion protein enables light-inducible allosteric control of enzymatic activity. J Biol Chem, 2023
21. Engineering of bidirectional, cyanobacteriochrome-based light-inducible dimers (BICYCL)s. Nat Methods, 2023
22. Crosstalk between Rac and Rho GTPase activity mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles. bioRxiv, 2023
23. LILAC: enhanced actin imaging with an optogenetic Lifeact. Nat Methods, 2023
24. Optogenetic dissection of transcriptional repression in a multicellular organism. bioRxiv, 2022
25. Analysis of Slow-Cycling Variants of the Light-Inducible Nuclear Protein Export System LEXY in Mammalian Cells. ACS Synth Biol, 2022
26. Optogenetic control of RelA reveals effect of transcription factor dynamics on downstream gene expression. bioRxiv, 2022
27. A genetically encoded photo-proximity labeling approach for mapping protein territories. bioRxiv, 2022
28. Optogenetic control of YAP cellular localisation and function. EMBO Rep, 2022
29. Two Rac1 pools integrate the direction and coordination of collective cell migration. bioRxiv, 2022
30. LILAC: Enhanced actin imaging with an optogenetic Lifeact. bioRxiv, 2022
31. Optical control of protein delivery and partitioning in the nucleolus. Nucleic Acids Res, 2022
32. Local temporal Rac1-GTP nadirs and peaks restrict cell protrusions and retractions. Sci Adv, 2022
33. Optogenetic control of the Bicoid morphogen reveals fast and slow modes of gap gene regulation. Cell Rep, 2022
34. MYC amplifies gene expression through global changes in transcription factor dynamics. Cell Rep, 2022
35. Quantification of nuclear transport inhibition by SARS-CoV-2 ORF6 using a broadly applicable live-cell dose-response pipeline. bioRxiv, 2021
36. Analysis of Three Architectures for Controlling PTP1B with Light. ACS Synth Biol, 2021
37. Two-input protein logic gate for computation in living cells. Nat Commun, 2021
38. Spatial and temporal control of expression with light-gated LOV-LexA. bioRxiv, 2021
39. Requirements for mammalian promoters to decode transcription factor dynamics. bioRxiv, 2021
40. Desensitisation of Notch signalling through dynamic adaptation in the nucleus. EMBO J, 2021
41. Mechanical worrying drives cell migration in crowded environments. bioRxiv, 2021
42. Extremely rapid and reversible optogenetic perturbation of nuclear proteins in living embryos. Dev Cell, 2021
43. Mechanosensitivity of nucleocytoplasmic transport. bioRxiv, 2021
44. Circularly permuted AsLOV2 as an optogenetic module for engineering photoswitchable peptides. Chem Commun (Camb), 2021
45. Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells. J Vis Exp, 2021
46. O-GlcNAc modification of nuclear pore complexes accelerates bidirectional transport J Cell Biol, 2021
47. Engineering a Blue Light Inducible SpyTag System (BLISS). J Am Chem Soc, 2021
48. Rab10-Positive Tubular Structures Represent a Novel Endocytic Pathway That Diverges From Canonical Macropinocytosis in RAW264 Macrophages. Front Immunol, 2021
49. A photo-switchable yeast isocitrate dehydrogenase to control metabolic flux through the citric acid cycle. bioRxiv, 2021
50. Light-dependent N-end rule-mediated disruption of protein function in Saccharomyces cerevisiae and Drosophila melanogaster. PLoS Genet, 2021
51. Circularly permuted LOV2 as a modular photoswitch for optogenetic engineering. Nat Chem Biol, 2021
52. Rac1 activation can generate untemplated, lamellar membrane ruffles. BMC Biol, 2021
53. Optogenetic control of Neisseria meningitidis Cas9 genome editing using an engineered, light-switchable anti-CRISPR protein. Nucleic Acids Res, 2021
54. Optogenetic control of calcium influx in mammalian cells. Methods Enzymol, 2021
55. Structural Determinants for Light-Dependent Membrane Binding of a Photoswitchable Polybasic Domain. ACS Synth Biol, 2021
56. A single-chain and fast-responding light-inducible Cre recombinase as a novel optogenetic switch. Elife, 2021
57. Blue Light‐Operated CRISPR/Cas13b‐Mediated mRNA Knockdown (Lockdown). Adv Biol, 2021
58. Optogenetic Control of Myocardin‐Related Transcription Factor A Subcellular Localization and Transcriptional Activity Steers Membrane Blebbing and Invasive Cancer Cell Motility. Adv Biol, 2021
59. Design of Smart Antibody Mimetics with Photosensitive Switches. Adv Biol (Weinh), 2021
60. High levels of Dorsal transcription factor downregulate, not promote, snail expression by regulating enhancer action. bioRxiv, 2021
61. Engineering of a bona fide light-operated calcium channel. Nat Commun, 2021
62. Design of smart antibody mimetics with photosensitive switches. bioRxiv, 2020
63. A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics. Elife, 2020
64. Livecell reporters reveal bidirectional acceleration of nucleocytoplasmic transport by O-GlcNAc modification of the nuclear pore complex. bioRxiv, 2020
65. An optogenetic switch for the Set2 methyltransferase provides evidence for transcription-dependent and -independent dynamics of H3K36 methylation. Genome Res, 2020
66. Optogenetic activation of heterotrimeric G-proteins by LOV2GIVe, a rationally engineered modular protein. Elife, 2020
67. Optogenetic Control Reveals Differential Promoter Interpretation of Transcription Factor Nuclear Translocation Dynamics. Cell Syst, 2020
68. Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold. Nat Commun, 2020
69. Optogenetic control of protein binding using light-switchable nanobodies. Nat Commun, 2020
70. Photo-SNAP-tag, a Light-Regulated Chemical Labeling System. ACS Chem Biol, 2020
71. Optogenetic Downregulation of Protein Levels to Control Programmed Cell Death in Mammalian Cells with a Dual Blue-Light Switch. Methods Mol Biol, 2020
72. Optogenetic Control of Nucleocytoplasmic Protein Transport. Methods Mol Biol, 2020
73. Light-Inducible CRISPR Labeling. Methods Mol Biol, 2020
74. Long-Range Optogenetic Control of Axon Guidance Overcomes Developmental Boundaries and Defects. Dev Cell, 2020
75. Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron. Genes Dev, 2020
76. Combining optogenetics with sensitive FRET imaging to monitor local microtubule manipulations. Sci Rep, 2020
77. The histone H4 basic patch regulates SAGA-mediated H2B deubiquitylation and histone acetylation. J Biol Chem, 2020
78. Optogenetic Control for Investigating Subcellular Localization of Fyn Kinase Activity in Single Live Cells. J Mol Biol, 2020
79. Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling. Nat Commun, 2020
80. Engineering light-controllable CAR T cells for cancer immunotherapy. Sci Adv, 2020
81. Optogenetic Rac1 engineered from membrane lipid-binding RGS-LOV for inducible lamellipodia formation. Photochem Photobiol Sci, 2020
82. Minimally disruptive optical control of protein tyrosine phosphatase 1B. Nat Commun, 2020
83. Optogenetic regulation of endogenous proteins. Nat Commun, 2020
84. Golgi-associated microtubules are fast cargo tracks and required for persistent cell migration. EMBO Rep, 2020
85. Optogenetic control of spine-head JNK reveals a role in dendritic spine regression. eNeuro, 2020
86. Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms. Nat Cell Biol, 2019
87. Directed evolution improves the catalytic efficiency of TEV protease. Nat Methods, 2019
88. Visualization of a blue light transmission area in living animals using light-induced nuclear translocation of fluorescent proteins. Biochem Biophys Res Commun, 2019
89. Locally Activating TrkB Receptor Generates Actin Waves and Specifies Axonal Fate. Cell Chem Biol, 2019
90. Chemokine Receptor CCR7 Triggers an Endomembrane Signaling Complex for Spatial Rac Activation. Cell Rep, 2019
91. Repurposing protein degradation for optogenetic modulation of protein activities. ACS Synth Biol, 2019
92. Optogenetic Repressors of Gene Expression in Yeasts Using Light-Controlled Nuclear Localization. CMBE, 2019
93. FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics. Nat Methods, 2019
94. Coordination of protrusion dynamics within and between collectively migrating border cells by myosin II. Mol Biol Cell, 2019
95. Reversible photocontrol of oxidase activity by inserting a photosensitive domain into the oxidase. BIOB, 2019
96. VIEW-MOD: a versatile illumination engine with a modular optical design for fluorescence microscopy. Opt Express, 2019
97. Optical control of transcription - genetically encoded photoswitchable variants of T7 RNA polymerase. Chembiochem, 2019
98. Engineering Optogenetic Control of Endogenous p53 Protein Levels. Appl Sci, 2019
99. Engineering proteins for allosteric control by light or ligands. Nat Protoc, 2019
100. Optogenetic downregulation of protein levels with an ultrasensitive switch. ACS Synth Biol, 2019
101. Optogenetic manipulation of stomatal kinetics improves carbon assimilation, water use, and growth. Science, 2019
102. A yeast system for discovering optogenetic inhibitors of eukaryotic translation initiation. ACS Synth Biol, 2019
103. Optogenetic control of cell morphogenesis on protein micropatterns. bioRxiv, 2019
104. Optogenetic Navigation of Routes Leading to Protein Amyloidogenesis in Bacteria. J Mol Biol, 2019
105. Photo‐ECM: A Blue Light Photoswitchable Synthetic Extracellular Matrix Protein for Reversible Control over Cell–Matrix Adhesion. Adv Biosyst, 2019
106. Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing. Nat Commun, 2019
107. Engineering Improved Photoswitches for the Control of Nucleocytoplasmic Distribution. ACS Synth Biol, 2018
108. Integrating chemical and mechanical signals through dynamic coupling between cellular protrusions and pulsed ERK activation. Nat Commun, 2018
109. Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. Nat Methods, 2018
110. A light-gated potassium channel for sustained neuronal inhibition. Nat Methods, 2018
111. Optogenetic manipulation of intracellular calcium by BACCS promotes differentiation of MC3T3-E1 cells. Biochem Biophys Res Commun, 2018
112. Cyclic Stiffness Modulation of Cell‐Laden Protein–Polymer Hydrogels in Response to User‐Specified Stimuli Including Light. Adv Biosyst, 2018
113. Dual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells. Sci Rep, 2018
114. Fam49/CYRI interacts with Rac1 and locally suppresses protrusions. Nat Cell Biol, 2018
115. Discovering selective binders for photoswitchable proteins using phage display. ACS Synth Biol, 2018
116. Near-infrared light-controlled systems for gene transcription regulation, protein targeting and spectral multiplexing. Nat Protoc, 2018
117. A Rac1-FMNL2 signaling module affects cell-cell contact formation independent of Cdc42 and membrane protrusions. PLoS ONE, 2018
118. Rewiring Calcium Signaling for Precise Transcriptional Reprogramming. ACS Synth Biol, 2018
119. Near-infrared light-controlled gene expression and protein targeting in neurons and non-neuronal cells. Chembiochem, 2018
120. Time-gated detection of protein-protein interactions with transcriptional readout. Elife, 2017
121. Engineering a light-activated caspase-3 for precise ablation of neurons in vivo. Proc Natl Acad Sci USA, 2017
122. An engineered photoswitchable mammalian pyruvate kinase. FEBS J, 2017
123. A calcium- and light-gated switch to induce gene expression in activated neurons. Nat Biotechnol, 2017
124. A light- and calcium-gated transcription factor for imaging and manipulating activated neurons. Nat Biotechnol, 2017
125. Rac1 switching at the right time and location is essential for Fcγ receptor-mediated phagosome formation. J Cell Sci, 2017
126. Optical control of membrane tethering and interorganellar communication at nanoscales. Chem Sci, 2017
127. A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat Commun, 2017
128. Light-induced protein degradation in human-derived cells. Biochem Biophys Res Commun, 2017
129. Near-infrared optogenetic pair for protein regulation and spectral multiplexing. Nat Chem Biol, 2017
130. Kinetics of Endogenous CaMKII Required for Synaptic Plasticity Revealed by Optogenetic Kinase Inhibitor. Neuron, 2017
131. Investigations of human myosin VI targeting using optogenetically controlled cargo loading. Proc Natl Acad Sci USA, 2017
132. Engineering extrinsic disorder to control protein activity in living cells. Science, 2016
133. Optogenetic Control of Nuclear Protein Import in Living Cells Using Light-Inducible Nuclear Localization Signals (LINuS). Curr Protoc Chem Biol, 2016
134. Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol, 2016
135. Post-translational control of protein function with light using a LOV-intein fusion protein. Mol Biosyst, 2016
136. Optogenetic control of nuclear protein export. Nat Commun, 2016
137. Regulation of neural gene transcription by optogenetic inhibition of the RE1-silencing transcription factor. Proc Natl Acad Sci USA, 2015
138. Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation. Elife, 2015
139. Photocontrolled Exposure of Pro-apoptotic Peptide Sequences in LOV Proteins Modulates Bcl-2 Family Interactions. Chembiochem, 2015
140. Optical Control of Peroxisomal Trafficking. ACS Synth Biol, 2015
141. Optogenetic control of endogenous Ca(2+) channels in vivo. Nat Biotechnol, 2015
142. Labelling and optical erasure of synaptic memory traces in the motor cortex. Nature, 2015
143. An Engineered Split Intein for Photoactivated Protein Trans-Splicing. PLoS ONE, 2015
144. Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS. Nat Commun, 2015
145. Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation. PLoS ONE, 2015
146. Junctional actin assembly is mediated by Formin-like 2 downstream of Rac1. J Cell Biol, 2015
147. Optogenetics. Engineering of a light-gated potassium channel. Science, 2015
148. An optogenetic upgrade for the Tet-OFF system. Biotechnol Bioeng, 2015
149. Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins. Proc Natl Acad Sci USA, 2014
150. Structural details of light activation of the LOV2-based photoswitch PA-Rac1. ACS Chem Biol, 2014
151. Remote control of myosin and kinesin motors using light-activated gearshifting. Nat Nanotechnol, 2014
152. Engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells. Nat Commun, 2014
153. Manipulation of endogenous kinase activity in living cells using photoswitchable inhibitory peptides. ACS Synth Biol, 2014
154. Rac1-dependent lamellipodial motility in prostate cancer PC-3 cells revealed by optogenetic control of Rac1 activity. PLoS ONE, 2014
155. Optical control of the Ca2+ concentration in a live specimen with a genetically encoded Ca2+-releasing molecular tool. ACS Chem Biol, 2014
156. A fully genetically encoded protein architecture for optical control of peptide ligand concentration. Nat Commun, 2014
157. General method for regulating protein stability with light. ACS Chem Biol, 2013
158. RasGRF2 Rac-GEF activity couples NMDA receptor calcium flux to enhanced synaptic transmission. Proc Natl Acad Sci USA, 2013
159. An optogenetic tool for the activation of endogenous diaphanous-related formins induces thickening of stress fibers without an increase in contractility. Cytoskeleton (Hoboken), 2013
160. Nuclear actin network assembly by formins regulates the SRF coactivator MAL. Science, 2013
161. Light activated cell migration in synthetic extracellular matrices. Biomaterials, 2012
162. Controlling the DNA cleavage activity of light-inducible chimeric endonucleases by bidirectional photoactivation. Bioconjug Chem, 2012
163. Designing photoswitchable peptides using the AsLOV2 domain. Chem Biol, 2012
164. Engineering a photoactivated caspase-7 for rapid induction of apoptosis. ACS Synth Biol, 2011
165. A synthetic photoactivated protein to generate local or global Ca(2+) signals. Chem Biol, 2011
166. Rationally improving LOV domain-based photoswitches. Nat Methods, 2010
167. Light-mediated activation reveals a key role for Rac in collective guidance of cell movement in vivo. Nat Cell Biol, 2010
168. A genetically encoded photoactivatable Rac controls the motility of living cells. Nature, 2009
169. Surface sites for engineering allosteric control in proteins. Science, 2008
170. Light-activated DNA binding in a designed allosteric protein. Proc Natl Acad Sci USA, 2008
171. Rac1 is essential in cocaine-induced structural plasticity of nucleus accumbens neurons. Nat Neurosci, 2012
172. LOV2-Controlled Photoactivation of Protein Trans-Splicing. Methods Mol Biol, 2017
173. SPARK: A Transcriptional Assay for Recording Protein-Protein Interactions in a Defined Time Window. Curr Protoc, 2021