AsLOV2

Photoreceptor AsLOV2
Binding partner /
Cofactor FMN
Source organism Avena sativa
Mode of action intramolecular conformational change
Excitation wavelength 450 nm
Reversion wavelength dark
Excitation time seconds
Reversion time tens of seconds
Crystal structure of AsLOV2, ground state. (PDB ID: 2V1A)

AsLOV2-derived switches:

Related original publications

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  1. LILAC: Enhanced actin imaging with an optogenetic Lifeact. bioRxiv, 2022
  2. Optical control of protein delivery and partitioning in the nucleolus. Nucleic Acids Res, 2022
  3. Local temporal Rac1-GTP nadirs and peaks restrict cell protrusions and retractions. Sci Adv, 2022
  4. Optogenetic control of the Bicoid morphogen reveals fast and slow modes of gap gene regulation. Cell Rep, 2022
  5. MYC amplifies gene expression through global changes in transcription factor dynamics. Cell Rep, 2022
  6. Quantification of nuclear transport inhibition by SARS-CoV-2 ORF6 using a broadly applicable live-cell dose-response pipeline. bioRxiv, 2021
  7. Analysis of Three Architectures for Controlling PTP1B with Light. ACS Synth Biol, 2021
  8. Two-input protein logic gate for computation in living cells. Nat Commun, 2021
  9. Spatial and temporal control of expression with light-gated LOV-LexA. bioRxiv, 2021
  10. Requirements for mammalian promoters to decode transcription factor dynamics. bioRxiv, 2021
  11. Optogenetic control of the Bicoid morphogen reveals fast and slow modes of gap gene regulation. bioRxiv, 2021
  12. Desensitisation of Notch signalling through dynamic adaptation in the nucleus. EMBO J, 2021
  13. Mechanical worrying drives cell migration in crowded environments. bioRxiv, 2021
  14. Extremely rapid and reversible optogenetic perturbation of nuclear proteins in living embryos. Dev Cell, 2021
  15. Mechanosensitivity of nucleocytoplasmic transport. bioRxiv, 2021
  16. Circularly permuted AsLOV2 as an optogenetic module for engineering photoswitchable peptides. Chem Commun (Camb), 2021
  17. Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells. J Vis Exp, 2021
  18. O-GlcNAc modification of nuclear pore complexes accelerates bidirectional transport. J Cell Biol, 2021
  19. Engineering a Blue Light Inducible SpyTag System (BLISS). J Am Chem Soc, 2021
  20. Rab10-Positive Tubular Structures Represent a Novel Endocytic Pathway That Diverges From Canonical Macropinocytosis in RAW264 Macrophages. Front Immunol, 2021
  21. A photo-switchable yeast isocitrate dehydrogenase to control metabolic flux through the citric acid cycle. bioRxiv, 2021
  22. Light-dependent N-end rule-mediated disruption of protein function in Saccharomyces cerevisiae and Drosophila melanogaster. PLoS Genet, 2021
  23. Circularly permuted LOV2 as a modular photoswitch for optogenetic engineering. Nat Chem Biol, 2021
  24. Rac1 activation can generate untemplated, lamellar membrane ruffles. BMC Biol, 2021
  25. Optogenetic manipulation of YAP cellular localisation and function. bioRxiv, 2021
  26. Optogenetic control of Neisseria meningitidis Cas9 genome editing using an engineered, light-switchable anti-CRISPR protein. Nucleic Acids Res, 2021
  27. Optogenetic control of calcium influx in mammalian cells. Methods Enzymol, 2021
  28. Structural Determinants for Light-Dependent Membrane Binding of a Photoswitchable Polybasic Domain. ACS Synth Biol, 2021
  29. A single-chain and fast-responding light-inducible Cre recombinase as a novel optogenetic switch. Elife, 2021
  30. Blue Light‐Operated CRISPR/Cas13b‐Mediated mRNA Knockdown (Lockdown). Adv Biol, 2021
  31. Optogenetic Control of Myocardin‐Related Transcription Factor A Subcellular Localization and Transcriptional Activity Steers Membrane Blebbing and Invasive Cancer Cell Motility. Adv Biol, 2021
  32. Design of Smart Antibody Mimetics with Photosensitive Switches. Adv Biol (Weinh), 2021
  33. High levels of Dorsal transcription factor downregulate, not promote, snail expression by regulating enhancer action. bioRxiv, 2021
  34. Engineering of a bona fide light-operated calcium channel. Nat Commun, 2021
  35. Design of smart antibody mimetics with photosensitive switches. bioRxiv, 2020
  36. A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics. Elife, 2020
  37. Livecell reporters reveal bidirectional acceleration of nucleocytoplasmic transport by O-GlcNAc modification of the nuclear pore complex. bioRxiv, 2020
  38. An optogenetic switch for the Set2 methyltransferase provides evidence for transcription-dependent and -independent dynamics of H3K36 methylation. Genome Res, 2020
  39. Optogenetic activation of heterotrimeric G-proteins by LOV2GIVe, a rationally engineered modular protein. Elife, 2020
  40. Optogenetic Control Reveals Differential Promoter Interpretation of Transcription Factor Nuclear Translocation Dynamics. Cell Syst, 2020
  41. Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold. Nat Commun, 2020
  42. Optogenetic control of protein binding using light-switchable nanobodies. Nat Commun, 2020
  43. Photo-SNAP-tag, a Light-Regulated Chemical Labeling System. ACS Chem Biol, 2020
  44. Optogenetic Downregulation of Protein Levels to Control Programmed Cell Death in Mammalian Cells with a Dual Blue-Light Switch. Methods Mol Biol, 2020
  45. Optogenetic Control of Nucleocytoplasmic Protein Transport. Methods Mol Biol, 2020
  46. Light-Inducible CRISPR Labeling. Methods Mol Biol, 2020
  47. Long-Range Optogenetic Control of Axon Guidance Overcomes Developmental Boundaries and Defects. Dev Cell, 2020
  48. Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron. Genes Dev, 2020
  49. Combining optogenetics with sensitive FRET imaging to monitor local microtubule manipulations. Sci Rep, 2020
  50. The histone H4 basic patch regulates SAGA-mediated H2B deubiquitylation and histone acetylation. J Biol Chem, 2020
  51. Optogenetic Control for Investigating Subcellular Localization of Fyn Kinase Activity in Single Live Cells. J Mol Biol, 2020
  52. Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling. Nat Commun, 2020
  53. Engineering light-controllable CAR T cells for cancer immunotherapy. Sci Adv, 2020
  54. Optogenetic Rac1 engineered from membrane lipid-binding RGS-LOV for inducible lamellipodia formation. Photochem Photobiol Sci, 2020
  55. Minimally disruptive optical control of protein tyrosine phosphatase 1B. Nat Commun, 2020
  56. Optogenetic regulation of endogenous proteins. Nat Commun, 2020
  57. Golgi-associated microtubules are fast cargo tracks and required for persistent cell migration. EMBO Rep, 2020
  58. Optogenetic control of spine-head JNK reveals a role in dendritic spine regression. eNeuro, 2020
  59. Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms. Nat Cell Biol, 2019
  60. Directed evolution improves the catalytic efficiency of TEV protease. Nat Methods, 2019
  61. Visualization of a blue light transmission area in living animals using light-induced nuclear translocation of fluorescent proteins. Biochem Biophys Res Commun, 2019
  62. Locally Activating TrkB Receptor Generates Actin Waves and Specifies Axonal Fate. Cell Chem Biol, 2019
  63. Chemokine Receptor CCR7 Triggers an Endomembrane Signaling Complex for Spatial Rac Activation. Cell Rep, 2019
  64. Repurposing protein degradation for optogenetic modulation of protein activities. ACS Synth Biol, 2019
  65. Optogenetic Repressors of Gene Expression in Yeasts Using Light-Controlled Nuclear Localization. CMBE, 2019
  66. FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics. Nat Methods, 2019
  67. Coordination of protrusion dynamics within and between collectively migrating border cells by myosin II. Mol Biol Cell, 2019
  68. Reversible photocontrol of oxidase activity by inserting a photosensitive domain into the oxidase. BIOB, 2019
  69. VIEW-MOD: a versatile illumination engine with a modular optical design for fluorescence microscopy. Opt Express, 2019
  70. Optical control of transcription - genetically encoded photoswitchable variants of T7 RNA polymerase. Chembiochem, 2019
  71. Engineering Optogenetic Control of Endogenous p53 Protein Levels. Appl Sci, 2019
  72. Engineering proteins for allosteric control by light or ligands. Nat Protoc, 2019
  73. Optogenetic downregulation of protein levels with an ultrasensitive switch. ACS Synth Biol, 2019
  74. Optogenetic manipulation of stomatal kinetics improves carbon assimilation, water use, and growth. Science, 2019
  75. A yeast system for discovering optogenetic inhibitors of eukaryotic translation initiation. ACS Synth Biol, 2019
  76. Optogenetic control of cell morphogenesis on protein micropatterns. bioRxiv, 2019
  77. Optogenetic Navigation of Routes Leading to Protein Amyloidogenesis in Bacteria. J Mol Biol, 2019
  78. Photo‐ECM: A Blue Light Photoswitchable Synthetic Extracellular Matrix Protein for Reversible Control over Cell–Matrix Adhesion. Adv Biosyst, 2019
  79. Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing. Nat Commun, 2019
  80. Engineering Improved Photoswitches for the Control of Nucleocytoplasmic Distribution. ACS Synth Biol, 2018
  81. Integrating chemical and mechanical signals through dynamic coupling between cellular protrusions and pulsed ERK activation. Nat Commun, 2018
  82. Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. Nat Methods, 2018
  83. A light-gated potassium channel for sustained neuronal inhibition. Nat Methods, 2018
  84. Optogenetic manipulation of intracellular calcium by BACCS promotes differentiation of MC3T3-E1 cells. Biochem Biophys Res Commun, 2018
  85. Cyclic Stiffness Modulation of Cell‐Laden Protein–Polymer Hydrogels in Response to User‐Specified Stimuli Including Light. Adv Biosyst, 2018
  86. Dual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells. Sci Rep, 2018
  87. Fam49/CYRI interacts with Rac1 and locally suppresses protrusions. Nat Cell Biol, 2018
  88. Discovering selective binders for photoswitchable proteins using phage display. ACS Synth Biol, 2018
  89. Near-infrared light-controlled systems for gene transcription regulation, protein targeting and spectral multiplexing. Nat Protoc, 2018
  90. A Rac1-FMNL2 signaling module affects cell-cell contact formation independent of Cdc42 and membrane protrusions. PLoS ONE, 2018
  91. Rewiring Calcium Signaling for Precise Transcriptional Reprogramming. ACS Synth Biol, 2018
  92. Near-infrared light-controlled gene expression and protein targeting in neurons and non-neuronal cells. Chembiochem, 2018
  93. Time-gated detection of protein-protein interactions with transcriptional readout. Elife, 2017
  94. Engineering a light-activated caspase-3 for precise ablation of neurons in vivo. Proc Natl Acad Sci USA, 2017
  95. An engineered photoswitchable mammalian pyruvate kinase. FEBS J, 2017
  96. A calcium- and light-gated switch to induce gene expression in activated neurons. Nat Biotechnol, 2017
  97. A light- and calcium-gated transcription factor for imaging and manipulating activated neurons. Nat Biotechnol, 2017
  98. Rac1 switching at the right time and location is essential for Fcγ receptor-mediated phagosome formation. J Cell Sci, 2017
  99. Optical control of membrane tethering and interorganellar communication at nanoscales. Chem Sci, 2017
  100. A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat Commun, 2017
  101. Light-induced protein degradation in human-derived cells. Biochem Biophys Res Commun, 2017
  102. Near-infrared optogenetic pair for protein regulation and spectral multiplexing. Nat Chem Biol, 2017
  103. Kinetics of Endogenous CaMKII Required for Synaptic Plasticity Revealed by Optogenetic Kinase Inhibitor. Neuron, 2017
  104. Investigations of human myosin VI targeting using optogenetically controlled cargo loading. Proc Natl Acad Sci USA, 2017
  105. Engineering extrinsic disorder to control protein activity in living cells. Science, 2016
  106. Optogenetic Control of Nuclear Protein Import in Living Cells Using Light-Inducible Nuclear Localization Signals (LINuS). Curr Protoc Chem Biol, 2016
  107. Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol, 2016
  108. Post-translational control of protein function with light using a LOV-intein fusion protein. Mol Biosyst, 2016
  109. Optogenetic control of nuclear protein export. Nat Commun, 2016
  110. Regulation of neural gene transcription by optogenetic inhibition of the RE1-silencing transcription factor. Proc Natl Acad Sci USA, 2015
  111. Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation. Elife, 2015
  112. Photocontrolled Exposure of Pro-apoptotic Peptide Sequences in LOV Proteins Modulates Bcl-2 Family Interactions. Chembiochem, 2015
  113. Optical Control of Peroxisomal Trafficking. ACS Synth Biol, 2015
  114. Optogenetic control of endogenous Ca(2+) channels in vivo. Nat Biotechnol, 2015
  115. Labelling and optical erasure of synaptic memory traces in the motor cortex. Nature, 2015
  116. An Engineered Split Intein for Photoactivated Protein Trans-Splicing. PLoS ONE, 2015
  117. Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS. Nat Commun, 2015
  118. Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation. PLoS ONE, 2015
  119. Junctional actin assembly is mediated by Formin-like 2 downstream of Rac1. J Cell Biol, 2015
  120. Optogenetics. Engineering of a light-gated potassium channel. Science, 2015
  121. An optogenetic upgrade for the Tet-OFF system. Biotechnol Bioeng, 2015
  122. Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins. Proc Natl Acad Sci USA, 2014
  123. Structural details of light activation of the LOV2-based photoswitch PA-Rac1. ACS Chem Biol, 2014
  124. Remote control of myosin and kinesin motors using light-activated gearshifting. Nat Nanotechnol, 2014
  125. Engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells. Nat Commun, 2014
  126. Manipulation of endogenous kinase activity in living cells using photoswitchable inhibitory peptides. ACS Synth Biol, 2014
  127. Rac1-dependent lamellipodial motility in prostate cancer PC-3 cells revealed by optogenetic control of Rac1 activity. PLoS ONE, 2014
  128. Optical control of the Ca2+ concentration in a live specimen with a genetically encoded Ca2+-releasing molecular tool. ACS Chem Biol, 2014
  129. A fully genetically encoded protein architecture for optical control of peptide ligand concentration. Nat Commun, 2014
  130. General method for regulating protein stability with light. ACS Chem Biol, 2013
  131. RasGRF2 Rac-GEF activity couples NMDA receptor calcium flux to enhanced synaptic transmission. Proc Natl Acad Sci USA, 2013
  132. An optogenetic tool for the activation of endogenous diaphanous-related formins induces thickening of stress fibers without an increase in contractility. Cytoskeleton (Hoboken), 2013
  133. Nuclear actin network assembly by formins regulates the SRF coactivator MAL. Science, 2013
  134. Light activated cell migration in synthetic extracellular matrices. Biomaterials, 2012
  135. Controlling the DNA cleavage activity of light-inducible chimeric endonucleases by bidirectional photoactivation. Bioconjug Chem, 2012
  136. Designing photoswitchable peptides using the AsLOV2 domain. Chem Biol, 2012
  137. Engineering a photoactivated caspase-7 for rapid induction of apoptosis. ACS Synth Biol, 2011
  138. A synthetic photoactivated protein to generate local or global Ca(2+) signals. Chem Biol, 2011
  139. Rationally improving LOV domain-based photoswitches. Nat Methods, 2010
  140. Light-mediated activation reveals a key role for Rac in collective guidance of cell movement in vivo. Nat Cell Biol, 2010
  141. A genetically encoded photoactivatable Rac controls the motility of living cells. Nature, 2009
  142. Surface sites for engineering allosteric control in proteins. Science, 2008
  143. Light-activated DNA binding in a designed allosteric protein. Proc Natl Acad Sci USA, 2008
  144. Rac1 is essential in cocaine-induced structural plasticity of nucleus accumbens neurons. Nat Neurosci, 2012
  145. LOV2-Controlled Photoactivation of Protein Trans-Splicing. Methods Mol Biol, 2017
  146. SPARK: A Transcriptional Assay for Recording Protein-Protein Interactions in a Defined Time Window. Curr Protoc, 2021