iLID

Photoreceptor AsLOV2–SsrA
Binding partner SspB
Cofactor FMN
Source organism Avena sativa
Mode of action heterodimerization
Excitation wavelength 450 nm
Reversion wavelength dark
Excitation time seconds
Reversion time seconds to minutes (variants available)
Crystal Structure of the iLID heterodimer. (PDB ID: 4WF0)

Related original publications

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  1. Clustering-based positive feedback between a kinase and its substrate enables effective T-cell receptor signaling. bioRxiv, 2020
  2. Endothelial cell invasiveness is controlled by myosin IIA-dependent inhibition of Arp2/3 activity. bioRxiv, 2020
  3. Optimized iLID membrane anchors for local optogenetic protein recruitment. bioRxiv, 2020
  4. Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold. Nat Commun, 2020
  5. Optogenetic control of protein binding using light-switchable nanobodies. Nat Commun, 2020
  6. Engineering combinatorial and dynamic decoders using synthetic immediate-early genes. Commun Biol, 2020
  7. Dynamic centriolar localization of Polo and Centrobin in early mitosis primes centrosome asymmetry. PLoS Biol, 2020
  8. Photo-SNAP-tag, a Light-Regulated Chemical Labeling System. ACS Chem Biol, 2020
  9. LITESEC-T3SS - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution. Nat Commun, 2020
  10. Photoactivatable Cre recombinase 3.0 for in vivo mouse applications. Nat Commun, 2020
  11. Construction of Light-Activated Neurotrophin Receptors Using the Improved Light-Induced Dimerizer (iLID). J Mol Biol, 2020
  12. An optimized toolbox for the optogenetic control of intracellular transport. J Cell Biol, 2020
  13. Implementing Optogenetic Modulation in Mechanotransduction. Phys Rev X, 2020
  14. ActuAtor, a molecular tool for generating force in living cells: Controlled deformation of intracellular structures. bioRxiv, 2020
  15. Light-inducible generation of membrane curvature in live cells with engineered BAR domain proteins. ACS Synth Biol, 2020
  16. Optogenetic control of PRC1 reveals its role in chromosome alignment at the metaphase plate. bioRxiv, 2020
  17. A Live-Cell Screen for Altered Erk Dynamics Reveals Principles of Proliferative Control. Cell Syst, 2020
  18. SRRF-stream imaging of optogenetically controlled furrow formation shows localized and coordinated endocytosis and exocytosis mediating membrane remodeling. ACS Synth Biol, 2020
  19. Rapid Dynamics of Signal-Dependent Transcriptional Repression by Capicua. Dev Cell, 2020
  20. Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling. Nat Commun, 2020
  21. Tunable light and drug induced depletion of target proteins. Nat Commun, 2020
  22. Dynamic organelle distribution initiates actin-based spindle migration in mouse oocytes. Nat Commun, 2020
  23. Ras acts as a molecular switch between two forms of consolidated memory in Drosophila. Proc Natl Acad Sci USA, 2020
  24. Engineered BRET-Based Biologic Light Sources Enable Spatiotemporal Control over Diverse Optogenetic Systems. ACS Synth Biol, 2019
  25. Optimizing photoswitchable MEK. Proc Natl Acad Sci USA, 2019
  26. The importance of cell-cell interaction dynamics in bottom-up tissue engineering: Concepts of colloidal self-assembly in the fabrication of multicellular architectures. Nano Lett, 2019
  27. Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins. Nat Methods, 2019
  28. Optogenetic rescue of a developmental patterning mutant. bioRxiv, 2019
  29. Light controlled cell-to-cell adhesion and chemical communication in minimal synthetic cells. Chem Commun (Camb), 2019
  30. High-throughput multicolor optogenetics in microwell plates. Nat Protoc, 2019
  31. Optogenetic downregulation of protein levels with an ultrasensitive switch. ACS Synth Biol, 2019
  32. Reversible induction of mitophagy by an optogenetic bimodular system. Nat Commun, 2019
  33. Physical Plasma Membrane Perturbation Using Subcellular Optogenetics Drives Integrin-Activated Cell Migration. ACS Synth Biol, 2019
  34. Signaling Dynamics Control Cell Fate in the Early Drosophila Embryo. Dev Cell, 2019
  35. Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds. Cell, 2018
  36. Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome. Cell, 2018
  37. Light-Guided Motility of a Minimal Synthetic Cell. Nano Lett, 2018
  38. Increasing spatial resolution of photoregulated GTPases through immobilized peripheral membrane proteins. Small GTPases, 2018
  39. Reversible Social Self-Sorting of Colloidal Cell-Mimics with Blue Light Switchable Proteins. ACS Synth Biol, 2018
  40. Membrane Flow Drives an Adhesion-Independent Amoeboid Cell Migration Mode. Dev Cell, 2018
  41. Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble. Elife, 2018
  42. Optogenetic reversible knocksideways, laser ablation, and photoactivation on the mitotic spindle in human cells. Methods Cell Biol, 2018
  43. Optogenetic Control of Cell Migration. Methods Mol Biol, 2018
  44. Light-activated protein interaction with high spatial subcellular confinement. Proc Natl Acad Sci USA, 2018
  45. Dynamic blue light-switchable protein patterns on giant unilamellar vesicles. Chem Commun (Camb), 2018
  46. Control of microtubule dynamics using an optogenetic microtubule plus end-F-actin cross-linker. J Cell Biol, 2017
  47. Optogenetic Control of Endoplasmic Reticulum-Mitochondria Tethering. ACS Synth Biol, 2017
  48. Time-gated detection of protein-protein interactions with transcriptional readout. Elife, 2017
  49. Intracellular production of hydrogels and synthetic RNA granules by multivalent molecular interactions. Nat Mater, 2017
  50. Vesicle Docking Is a Key Target of Local PI(4,5)P2 Metabolism in the Secretory Pathway of INS-1 Cells. Cell Rep, 2017
  51. Photocontrolled reversible self-assembly of dodecamer nitrilase. Bioresour Bioprocess, 2017
  52. Cells lay their own tracks: optogenetic Cdc42 activation stimulates fibronectin deposition supporting directed migration. J Cell Sci, 2017
  53. Evolution of a split RNA polymerase as a versatile biosensor platform. Nat Chem Biol, 2017
  54. The Spatiotemporal Limits of Developmental Erk Signaling. Dev Cell, 2017
  55. Tuning the Binding Affinities and Reversion Kinetics of a Light Inducible Dimer Allows Control of Transmembrane Protein Localization. Biochemistry, 2016
  56. Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol, 2016
  57. Subcellular optogenetic activation of Cdc42 controls local and distal signaling to drive immune cell migration. Mol Biol Cell, 2016
  58. Correlating in Vitro and in Vivo Activities of Light-Inducible Dimers: A Cellular Optogenetics Guide. ACS Synth Biol, 2015
  59. Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins. Proc Natl Acad Sci USA, 2014