iLID

Photoreceptor AsLOV2–SsrA
Binding partner SspB
Cofactor FMN
Source organism Avena sativa
Mode of action heterodimerization
Excitation wavelength 450 nm
Reversion wavelength dark
Excitation time seconds
Reversion time seconds to minutes (variants available)
Crystal Structure of the iLID heterodimer. (PDB ID: 4WF0)

Related original publications

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  1. Opto-RhoGEFs: an optimized optogenetic toolbox to reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier strength. bioRxiv, 2022
  2. Defunctionalizing intracellular organelles such as mitochondria and peroxisomes with engineered phospholipase A/acyltransferases. Nat Commun, 2022
  3. Visual detection of submicroscopic protein clusters with a phase-separation-based fluorescent reporter. bioRxiv, 2022
  4. Dimerization of iLID Optogenetic Proteins Observed Using 3D Single-Molecule Tracking in Live Bacterial Cells. bioRxiv, 2022
  5. Synthetic cells with self-activating optogenetic proteins communicate with natural cells. Nat Commun, 2022
  6. Motor processivity and speed determine structure and dynamics of microtubule-motor assemblies. bioRxiv, 2022
  7. mTORC2 coordinates the leading and trailing edge cytoskeletal programs during neutrophil migration. bioRxiv, 2022
  8. Persistent cell migration emerges from a coupling between protrusion dynamics and polarized trafficking. Elife, 2022
  9. A rich get richer effect governs intracellular condensate size distributions. bioRxiv, 2022
  10. Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells. Front Cell Neurosci, 2022
  11. Oncogenic protein condensates modulate cell signal perception and drug tolerance. bioRxiv, 2022
  12. Compartmentalization of telomeres through DNA-scaffolded phase separation. Dev Cell, 2022
  13. Substratum stiffness regulates Erk signaling dynamics through receptor-level control. Cell Rep, 2021
  14. Opto-Katanin: An Optogenetic Tool for Localized Microtubule Disassembly. bioRxiv, 2021
  15. Temperature-responsive optogenetic probes of cell signaling. Nat Chem Biol, 2021
  16. Gezielte Injektion von Effektoren durch Kontrolle der Proteindynamik. BIOspektrum (Heidelb), 2021
  17. An Optogenetic Toolbox for Synergistic Regulation of Protein Abundance. ACS Synth Biol, 2021
  18. A synthetic gene circuit for imaging-free detection of signaling pulses. Cell Syst, 2021
  19. Nano-optogenetic engineering of CAR T cells for precision immunotherapy with enhanced safety. Nat Nanotechnol, 2021
  20. Defunctionalizing Intracellular Organelles with Genetically-Encoded Molecular Tools Based on Engineered Phospholipase A/Acyltransferases (PLAATs). bioRxiv, 2021
  21. An active tethering mechanism controls the fate of vesicles. Nat Commun, 2021
  22. A Non-Canonical Raf Function Is Required for Dorsal-Ventral Patterning During Drosophila Embryogenesis. bioRxiv, 2021
  23. Circularly permuted AsLOV2 as an optogenetic module for engineering photoswitchable peptides. Chem Commun (Camb), 2021
  24. Mechanical Frustration of Phase Separation in the Cell Nucleus by Chromatin. Phys Rev Lett, 2021
  25. Positive feedback between the T cell kinase Zap70 and its substrate LAT acts as a clustering-dependent signaling switch. Cell Rep, 2021
  26. Cell to Cell Signaling through Light in Artificial Cell Communities: Glowing Predator Lures Prey. ACS Nano, 2021
  27. mem-iLID, a fast and economic protein purification method. Biosci Rep, 2021
  28. Optogenetic model reveals cell shape regulation through FAK and Fascin. J Cell Sci, 2021
  29. Temporal integration of inductive cues on the way to gastrulation. Proc Natl Acad Sci U S A, 2021
  30. Light-inducible deformation of mitochondria in live cells. Cell Chem Biol, 2021
  31. Engineering a Blue Light Inducible SpyTag System (BLISS). J Am Chem Soc, 2021
  32. Bioluminescent Synthetic Cells Communicate with Natural Cells and Self-Activate Light-Responsive Proteins. bioRxiv, 2021
  33. Circularly permuted LOV2 as a modular photoswitch for optogenetic engineering. Nat Chem Biol, 2021
  34. Endothelial cell invasion is controlled by dactylopodia. Proc Natl Acad Sci U S A, 2021
  35. Dynamics and heterogeneity of Erk-induced immediate-early gene expression. bioRxiv, 2021
  36. Optogenetic control of apical constriction induces synthetic morphogenesis in mammalian tissues. bioRxiv, 2021
  37. Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis. bioRxiv, 2021
  38. Optimized iLID Membrane Anchors for Local Optogenetic Protein Recruitment. ACS Synth Biol, 2021
  39. Coupling between protrusion dynamics and polarized trafficking steers persistent cell migration. bioRxiv, 2021
  40. T cells selectively filter oscillatory signals on the minutes timescale. Proc Natl Acad Sci U S A, 2021
  41. Optogenetic control of PRC1 reveals its role in chromosome alignment on the spindle by overlap length-dependent forces. Elife, 2021
  42. Optogenetic control of small GTPases reveals RhoA mediates intracellular calcium signaling. J Biol Chem, 2021
  43. Optogenetic inhibition and activation of Rac and Rap1 using a modified iLID system. bioRxiv, 2020
  44. Rho1 activation recapitulates early gastrulation events in the ventral, but not dorsal, epithelium of Drosophila embryos. Elife, 2020
  45. Light-inducible Deformation of Mitochondria in Live Cells. bioRxiv, 2020
  46. Clustering-based positive feedback between a kinase and its substrate enables effective T-cell receptor signaling. bioRxiv, 2020
  47. Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold. Nat Commun, 2020
  48. Optogenetic control of protein binding using light-switchable nanobodies. Nat Commun, 2020
  49. Engineering combinatorial and dynamic decoders using synthetic immediate-early genes. Commun Biol, 2020
  50. Multistimuli Sensing Adhesion Unit for the Self-Positioning of Minimal Synthetic Cells. Small, 2020
  51. Dynamic centriolar localization of Polo and Centrobin in early mitosis primes centrosome asymmetry. PLoS Biol, 2020
  52. Heterogeneous somatostatin-expressing neuron population in mouse ventral tegmental area. Elife, 2020
  53. Photo-SNAP-tag, a Light-Regulated Chemical Labeling System. ACS Chem Biol, 2020
  54. LITESEC-T3SS - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution. Nat Commun, 2020
  55. Photoactivatable Cre recombinase 3.0 for in vivo mouse applications. Nat Commun, 2020
  56. Construction of Light-Activated Neurotrophin Receptors Using the Improved Light-Induced Dimerizer (iLID). J Mol Biol, 2020
  57. An optimized toolbox for the optogenetic control of intracellular transport. J Cell Biol, 2020
  58. Implementing Optogenetic Modulation in Mechanotransduction. Phys Rev X, 2020
  59. ActuAtor, a molecular tool for generating force in living cells: Controlled deformation of intracellular structures. bioRxiv, 2020
  60. Light-inducible generation of membrane curvature in live cells with engineered BAR domain proteins. ACS Synth Biol, 2020
  61. A Live-Cell Screen for Altered Erk Dynamics Reveals Principles of Proliferative Control. Cell Syst, 2020
  62. SRRF-stream imaging of optogenetically controlled furrow formation shows localized and coordinated endocytosis and exocytosis mediating membrane remodeling. ACS Synth Biol, 2020
  63. Rapid Dynamics of Signal-Dependent Transcriptional Repression by Capicua. Dev Cell, 2020
  64. Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling. Nat Commun, 2020
  65. Tunable light and drug induced depletion of target proteins. Nat Commun, 2020
  66. Dynamic organelle distribution initiates actin-based spindle migration in mouse oocytes. Nat Commun, 2020
  67. Ras acts as a molecular switch between two forms of consolidated memory in Drosophila. Proc Natl Acad Sci USA, 2020
  68. Engineered BRET-Based Biologic Light Sources Enable Spatiotemporal Control over Diverse Optogenetic Systems. ACS Synth Biol, 2019
  69. Optimizing photoswitchable MEK. Proc Natl Acad Sci USA, 2019
  70. The importance of cell-cell interaction dynamics in bottom-up tissue engineering: Concepts of colloidal self-assembly in the fabrication of multicellular architectures. Nano Lett, 2019
  71. Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins. Nat Methods, 2019
  72. Light controlled cell-to-cell adhesion and chemical communication in minimal synthetic cells. Chem Commun (Camb), 2019
  73. High-throughput multicolor optogenetics in microwell plates. Nat Protoc, 2019
  74. Optogenetic downregulation of protein levels with an ultrasensitive switch. ACS Synth Biol, 2019
  75. Reversible induction of mitophagy by an optogenetic bimodular system. Nat Commun, 2019
  76. Physical Plasma Membrane Perturbation Using Subcellular Optogenetics Drives Integrin-Activated Cell Migration. ACS Synth Biol, 2019
  77. Signaling Dynamics Control Cell Fate in the Early Drosophila Embryo. Dev Cell, 2019
  78. A Photoactivatable Botulinum Neurotoxin for Inducible Control of Neurotransmission. Neuron, 2019
  79. Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds. Cell, 2018
  80. Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome. Cell, 2018
  81. Light-Guided Motility of a Minimal Synthetic Cell. Nano Lett, 2018
  82. Increasing spatial resolution of photoregulated GTPases through immobilized peripheral membrane proteins. Small GTPases, 2018
  83. Reversible Social Self-Sorting of Colloidal Cell-Mimics with Blue Light Switchable Proteins. ACS Synth Biol, 2018
  84. Membrane Flow Drives an Adhesion-Independent Amoeboid Cell Migration Mode. Dev Cell, 2018
  85. Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble. Elife, 2018
  86. Optogenetic reversible knocksideways, laser ablation, and photoactivation on the mitotic spindle in human cells. Methods Cell Biol, 2018
  87. Optogenetic Control of Cell Migration. Methods Mol Biol, 2018
  88. Light-activated protein interaction with high spatial subcellular confinement. Proc Natl Acad Sci USA, 2018
  89. Dynamic blue light-switchable protein patterns on giant unilamellar vesicles. Chem Commun (Camb), 2018
  90. Control of microtubule dynamics using an optogenetic microtubule plus end-F-actin cross-linker. J Cell Biol, 2017
  91. Optogenetic Control of Endoplasmic Reticulum-Mitochondria Tethering. ACS Synth Biol, 2017
  92. Time-gated detection of protein-protein interactions with transcriptional readout. Elife, 2017
  93. Intracellular production of hydrogels and synthetic RNA granules by multivalent molecular interactions. Nat Mater, 2017
  94. Vesicle Docking Is a Key Target of Local PI(4,5)P2 Metabolism in the Secretory Pathway of INS-1 Cells. Cell Rep, 2017
  95. Photocontrolled reversible self-assembly of dodecamer nitrilase. Bioresour Bioprocess, 2017
  96. Cells lay their own tracks: optogenetic Cdc42 activation stimulates fibronectin deposition supporting directed migration. J Cell Sci, 2017
  97. Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain. Nat Methods, 2017
  98. Evolution of a split RNA polymerase as a versatile biosensor platform. Nat Chem Biol, 2017
  99. The Spatiotemporal Limits of Developmental Erk Signaling. Dev Cell, 2017
  100. Tuning the Binding Affinities and Reversion Kinetics of a Light Inducible Dimer Allows Control of Transmembrane Protein Localization. Biochemistry, 2016
  101. Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol, 2016
  102. Subcellular optogenetic activation of Cdc42 controls local and distal signaling to drive immune cell migration. Mol Biol Cell, 2016
  103. Correlating in Vitro and in Vivo Activities of Light-Inducible Dimers: A Cellular Optogenetics Guide. ACS Synth Biol, 2015
  104. Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins. Proc Natl Acad Sci USA, 2014
  105. Optogenetic actuator - ERK biosensor circuits identify MAPK network nodes that shape ERK dynamics. Mol Syst Biol, 2022