iLID

Photoreceptor AsLOV2–SsrA
Binding partner SspB
Cofactor FMN
Source organism Avena sativa
Mode of action heterodimerization
Excitation wavelength 450 nm
Reversion wavelength dark
Excitation time seconds
Reversion time seconds to minutes (variants available)
Crystal Structure of the iLID heterodimer. (PDB ID: 4WF0)

Related original publications

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  1. Endogenous OptoRhoGEFs reveal biophysical principles of epithelial tissue furrowing. bioRxiv, 2024
  2. Spatiotemporal Organization of Functional Cargoes by Light-Switchable Condensation in Escherichia coli Cells. JACS Au, 2024
  3. Dynamic Light-Induced Protein Patterns at Model Membranes. J Vis Exp, 2024
  4. Asymmetric oligomerization state and sequence patterning can tune multiphase condensate miscibility. Nat Chem, 2024
  5. Simple visualization of submicroscopic protein clusters with a phase-separation-based fluorescent reporter. Cell Syst, 2024
  6. Interplay of condensation and chromatin binding underlies BRD4 targeting. bioRxiv, 2024
  7. Using split protein reassembly strategy to optically control PLD enzymatic activity. bioRxiv, 2024
  8. Ultralow Background Membrane Editors for Spatiotemporal Control of Phosphatidic Acid Metabolism and Signaling ACS Cent Sci, 2024
  9. Rapid and reversible dissolution of biomolecular condensates using light-controlled recruitment of a solubility tag. bioRxiv, 2024
  10. Light-based juxtacrine signaling between synthetic cells. bioRxiv, 2024
  11. Photoactivation of LOV domains with chemiluminescence. Chem Sci, 2023
  12. A single-component, light-assisted uncaging switch for endoproteolytic release. Nat Chem Biol, 2023
  13. Critical capillary waves of biomolecular condensates. bioRxiv, 2023
  14. Local negative feedback of Rac activity at the leading edge underlies a pilot pseudopod-like program for amoeboid cell guidance. PLoS Biol, 2023
  15. CaaX-motif-adjacent residues influence G protein gamma (Gγ) prenylation under suboptimal conditions. J Biol Chem, 2023
  16. ActuAtor, a Listeria-inspired molecular tool for physical manipulation of intracellular organizations through de novo actin polymerization. Cell Rep, 2023
  17. Light-activated microtubule-based two-dimensional active nematic. Soft Matter, 2023
  18. Control of cell retraction and protrusion with a single protein. bioRxiv, 2023
  19. Dynamics of an incoherent feedforward loop drive ERK-dependent pattern formation in the early Drosophila embryo. Development, 2023
  20. C-terminal sequence stability profiling in Saccharomyces cerevisiae reveals protective protein quality control pathways. J Biol Chem, 2023
  21. Spatiotemporal optical control of Gαq-PLCβ interactions. bioRxiv, 2023
  22. Endoplasmic reticulum exit sites are segregated for secretion based on cargo size. bioRxiv, 2023
  23. Optogenetic clustering and membrane translocation of the BcLOV4 photoreceptor. Proc Natl Acad Sci U S A, 2023
  24. Opto-RhoGEFs, an optimized optogenetic toolbox to reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier strength. Elife, 2023
  25. Concept and considerations of a medical device: the active noise cancelling incubator. Front Pediatr, 2023
  26. Cell protrusions and contractions generate long-range membrane tension propagation. Cell, 2023
  27. Actuation of single downstream nodes in growth factor network steers immune cell migration. Dev Cell, 2023
  28. Engineered allostery in light-regulated LOV-Turbo enables precise spatiotemporal control of proximity labeling in living cells. Nat Methods, 2023
  29. Self-Regulated and Bidirectional Communication in Synthetic Cell Communities. ACS Nano, 2023
  30. Focal adhesions are controlled by microtubules through local contractility regulation. bioRxiv, 2023
  31. A disordered tether to iLID improves photoswitchable protein patterning on model membranes. Chem Commun (Camb), 2023
  32. Mechanosensitive mTORC2 independently coordinates leading and trailing edge polarity programs during neutrophil migration. Mol Biol Cell, 2023
  33. Motor processivity and speed determine structure and dynamics of microtubule-motor assemblies. Elife, 2023
  34. Rac negative feedback links local PIP3 rate-of-change to dynamic control of neutrophil guidance. bioRxiv, 2023
  35. Orthogonal Light-Dependent Membrane Adhesion Induces Social Self-Sorting and Member-Specific DNA Communication in Synthetic Cell Communities. Small, 2023
  36. Mechanistic insights into cancer drug resistance through optogenetic PI3K signaling hyperactivation. Cell Chem Biol, 2022
  37. Integrin-based adhesion compartmentalizes ALK3 of the BMPRII to control cell adhesion and migration. J Cell Biol, 2022
  38. Opto-katanin, an optogenetic tool for localized, microtubule disassembly. Curr Biol, 2022
  39. Optogenetic control of apical constriction induces synthetic morphogenesis in mammalian tissues. Nat Commun, 2022
  40. Defunctionalizing intracellular organelles such as mitochondria and peroxisomes with engineered phospholipase A/acyltransferases. Nat Commun, 2022
  41. Dimerization of iLID Optogenetic Proteins Observed Using 3D Single-Molecule Tracking in Live Bacterial Cells. bioRxiv, 2022
  42. A non-canonical Raf function is required for dorsal-ventral patterning during Drosophila embryogenesis. Sci Rep, 2022
  43. Synthetic cells with self-activating optogenetic proteins communicate with natural cells. Nat Commun, 2022
  44. mTORC2 coordinates the leading and trailing edge cytoskeletal programs during neutrophil migration. bioRxiv, 2022
  45. Persistent cell migration emerges from a coupling between protrusion dynamics and polarized trafficking. Elife, 2022
  46. A rich get richer effect governs intracellular condensate size distributions. bioRxiv, 2022
  47. Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells. Front Cell Neurosci, 2022
  48. Oncogenic protein condensates modulate cell signal perception and drug tolerance. bioRxiv, 2022
  49. Compartmentalization of telomeres through DNA-scaffolded phase separation. Dev Cell, 2022
  50. Substratum stiffness regulates Erk signaling dynamics through receptor-level control. Cell Rep, 2021
  51. Temperature-responsive optogenetic probes of cell signaling. Nat Chem Biol, 2021
  52. Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis. Nat Commun, 2021
  53. Gezielte Injektion von Effektoren durch Kontrolle der Proteindynamik. BIOspektrum (Heidelb), 2021
  54. An Optogenetic Toolbox for Synergistic Regulation of Protein Abundance. ACS Synth Biol, 2021
  55. A synthetic gene circuit for imaging-free detection of signaling pulses. Cell Syst, 2021
  56. Nano-optogenetic engineering of CAR T cells for precision immunotherapy with enhanced safety. Nat Nanotechnol, 2021
  57. An active tethering mechanism controls the fate of vesicles. Nat Commun, 2021
  58. Circularly permuted AsLOV2 as an optogenetic module for engineering photoswitchable peptides. Chem Commun (Camb), 2021
  59. Mechanical Frustration of Phase Separation in the Cell Nucleus by Chromatin. Phys Rev Lett, 2021
  60. Positive feedback between the T cell kinase Zap70 and its substrate LAT acts as a clustering-dependent signaling switch. Cell Rep, 2021
  61. Cell to Cell Signaling through Light in Artificial Cell Communities: Glowing Predator Lures Prey. ACS Nano, 2021
  62. mem-iLID, a fast and economic protein purification method. Biosci Rep, 2021
  63. Optogenetic model reveals cell shape regulation through FAK and Fascin. J Cell Sci, 2021
  64. Temporal integration of inductive cues on the way to gastrulation. Proc Natl Acad Sci U S A, 2021
  65. Light-inducible deformation of mitochondria in live cells. Cell Chem Biol, 2021
  66. Engineering a Blue Light Inducible SpyTag System (BLISS). J Am Chem Soc, 2021
  67. Bioluminescent Synthetic Cells Communicate with Natural Cells and Self-Activate Light-Responsive Proteins. bioRxiv, 2021
  68. Circularly permuted LOV2 as a modular photoswitch for optogenetic engineering. Nat Chem Biol, 2021
  69. Endothelial cell invasion is controlled by dactylopodia. Proc Natl Acad Sci U S A, 2021
  70. Dynamics and heterogeneity of Erk-induced immediate-early gene expression. bioRxiv, 2021
  71. Optimized iLID Membrane Anchors for Local Optogenetic Protein Recruitment. ACS Synth Biol, 2021
  72. T cells selectively filter oscillatory signals on the minutes timescale. Proc Natl Acad Sci U S A, 2021
  73. Optogenetic control of PRC1 reveals its role in chromosome alignment on the spindle by overlap length-dependent forces. Elife, 2021
  74. Optogenetic control of small GTPases reveals RhoA mediates intracellular calcium signaling. J Biol Chem, 2021
  75. Optogenetic inhibition and activation of Rac and Rap1 using a modified iLID system. bioRxiv, 2020
  76. Rho1 activation recapitulates early gastrulation events in the ventral, but not dorsal, epithelium of Drosophila embryos. Elife, 2020
  77. Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold. Nat Commun, 2020
  78. Optogenetic control of protein binding using light-switchable nanobodies. Nat Commun, 2020
  79. Engineering combinatorial and dynamic decoders using synthetic immediate-early genes. Commun Biol, 2020
  80. Multistimuli Sensing Adhesion Unit for the Self-Positioning of Minimal Synthetic Cells. Small, 2020
  81. Dynamic centriolar localization of Polo and Centrobin in early mitosis primes centrosome asymmetry. PLoS Biol, 2020
  82. Heterogeneous somatostatin-expressing neuron population in mouse ventral tegmental area. Elife, 2020
  83. Photo-SNAP-tag, a Light-Regulated Chemical Labeling System. ACS Chem Biol, 2020
  84. LITESEC-T3SS - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution. Nat Commun, 2020
  85. Photoactivatable Cre recombinase 3.0 for in vivo mouse applications. Nat Commun, 2020
  86. Construction of Light-Activated Neurotrophin Receptors Using the Improved Light-Induced Dimerizer (iLID). J Mol Biol, 2020
  87. An optimized toolbox for the optogenetic control of intracellular transport. J Cell Biol, 2020
  88. Implementing Optogenetic Modulation in Mechanotransduction. Phys Rev X, 2020
  89. Light-inducible generation of membrane curvature in live cells with engineered BAR domain proteins. ACS Synth Biol, 2020
  90. A Live-Cell Screen for Altered Erk Dynamics Reveals Principles of Proliferative Control. Cell Syst, 2020
  91. SRRF-stream imaging of optogenetically controlled furrow formation shows localized and coordinated endocytosis and exocytosis mediating membrane remodeling. ACS Synth Biol, 2020
  92. Rapid Dynamics of Signal-Dependent Transcriptional Repression by Capicua. Dev Cell, 2020
  93. Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling. Nat Commun, 2020
  94. Tunable light and drug induced depletion of target proteins. Nat Commun, 2020
  95. Dynamic organelle distribution initiates actin-based spindle migration in mouse oocytes. Nat Commun, 2020
  96. Ras acts as a molecular switch between two forms of consolidated memory in Drosophila. Proc Natl Acad Sci USA, 2020
  97. Engineered BRET-Based Biologic Light Sources Enable Spatiotemporal Control over Diverse Optogenetic Systems. ACS Synth Biol, 2019
  98. Optimizing photoswitchable MEK. Proc Natl Acad Sci USA, 2019
  99. The importance of cell-cell interaction dynamics in bottom-up tissue engineering: Concepts of colloidal self-assembly in the fabrication of multicellular architectures. Nano Lett, 2019
  100. Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins. Nat Methods, 2019
  101. Light controlled cell-to-cell adhesion and chemical communication in minimal synthetic cells. Chem Commun (Camb), 2019
  102. High-throughput multicolor optogenetics in microwell plates. Nat Protoc, 2019
  103. Optogenetic downregulation of protein levels with an ultrasensitive switch. ACS Synth Biol, 2019
  104. Reversible induction of mitophagy by an optogenetic bimodular system. Nat Commun, 2019
  105. Physical Plasma Membrane Perturbation Using Subcellular Optogenetics Drives Integrin-Activated Cell Migration. ACS Synth Biol, 2019
  106. Signaling Dynamics Control Cell Fate in the Early Drosophila Embryo. Dev Cell, 2019
  107. A Photoactivatable Botulinum Neurotoxin for Inducible Control of Neurotransmission. Neuron, 2019
  108. Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds. Cell, 2018
  109. Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome. Cell, 2018
  110. Light-Guided Motility of a Minimal Synthetic Cell. Nano Lett, 2018
  111. Increasing spatial resolution of photoregulated GTPases through immobilized peripheral membrane proteins. Small GTPases, 2018
  112. Reversible Social Self-Sorting of Colloidal Cell-Mimics with Blue Light Switchable Proteins. ACS Synth Biol, 2018
  113. Membrane Flow Drives an Adhesion-Independent Amoeboid Cell Migration Mode. Dev Cell, 2018
  114. Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble. Elife, 2018
  115. Optogenetic reversible knocksideways, laser ablation, and photoactivation on the mitotic spindle in human cells. Methods Cell Biol, 2018
  116. Optogenetic Control of Cell Migration. Methods Mol Biol, 2018
  117. Light-activated protein interaction with high spatial subcellular confinement. Proc Natl Acad Sci USA, 2018
  118. Dynamic blue light-switchable protein patterns on giant unilamellar vesicles. Chem Commun (Camb), 2018
  119. Control of microtubule dynamics using an optogenetic microtubule plus end-F-actin cross-linker. J Cell Biol, 2017
  120. Optogenetic Control of Endoplasmic Reticulum-Mitochondria Tethering. ACS Synth Biol, 2017
  121. Time-gated detection of protein-protein interactions with transcriptional readout. Elife, 2017
  122. Intracellular production of hydrogels and synthetic RNA granules by multivalent molecular interactions. Nat Mater, 2017
  123. Vesicle Docking Is a Key Target of Local PI(4,5)P2 Metabolism in the Secretory Pathway of INS-1 Cells. Cell Rep, 2017
  124. Photocontrolled reversible self-assembly of dodecamer nitrilase. Bioresour Bioprocess, 2017
  125. Cells lay their own tracks: optogenetic Cdc42 activation stimulates fibronectin deposition supporting directed migration. J Cell Sci, 2017
  126. Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain. Nat Methods, 2017
  127. Evolution of a split RNA polymerase as a versatile biosensor platform. Nat Chem Biol, 2017
  128. The Spatiotemporal Limits of Developmental Erk Signaling. Dev Cell, 2017
  129. Tuning the Binding Affinities and Reversion Kinetics of a Light Inducible Dimer Allows Control of Transmembrane Protein Localization. Biochemistry, 2016
  130. Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol, 2016
  131. Subcellular optogenetic activation of Cdc42 controls local and distal signaling to drive immune cell migration. Mol Biol Cell, 2016
  132. Correlating in Vitro and in Vivo Activities of Light-Inducible Dimers: A Cellular Optogenetics Guide. ACS Synth Biol, 2015
  133. Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins. Proc Natl Acad Sci USA, 2014
  134. Optogenetic actuator - ERK biosensor circuits identify MAPK network nodes that shape ERK dynamics. Mol Syst Biol, 2022
  135. Using Optogenetics to Spatially Control Cortical Dynein Activity in Mitotic Human Cells. Methods Mol Biol, 2023
  136. Application of Optogenetics to Probe the Signaling Dynamics of Cell Fate Decision-Making. Methods Mol Biol, 2023
  137. Nano-optogenetic CAR-T Cell Immunotherapy. Methods Mol Biol, 2024