iLID

Photoreceptor AsLOV2–SsrA
Binding partner SspB
Cofactor FMN
Source organism Avena sativa
Mode of action heterodimerization
Excitation wavelength 450 nm
Reversion wavelength dark
Excitation time seconds
Reversion time seconds to minutes (variants available)
Crystal Structure of the iLID heterodimer. (PDB ID: 4WF0)

Related original publications

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  1. Dynamic organelle distribution initiates actin-based spindle migration in mouse oocytes. Nat Commun, 2020
  2. Ras acts as a molecular switch between two forms of consolidated memory in Drosophila. Proc Natl Acad Sci USA, 2020
  3. Engineered BRET-Based Biologic Light Sources Enable Spatiotemporal Control over Diverse Optogenetic Systems. ACS Synth Biol, 2019
  4. Optimizing photoswitchable MEK. Proc Natl Acad Sci USA, 2019
  5. The importance of cell-cell interaction dynamics in bottom-up tissue engineering: Concepts of colloidal self-assembly in the fabrication of multicellular architectures. Nano Lett, 2019
  6. Construction of light-activated neurotrophin receptors using the improved Light-Induced Dimerizer (iLID) . bioRxiv, 2019
  7. Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins. Nat Methods, 2019
  8. Optogenetic rescue of a developmental patterning mutant. bioRxiv, 2019
  9. Light controlled cell-to-cell adhesion and chemical communication in minimal synthetic cells. Chem Commun (Camb), 2019
  10. High-throughput multicolor optogenetics in microwell plates. Nat Protoc, 2019
  11. A live-cell screen for altered Erk dynamics reveals principles of proliferative control. bioRxiv, 2019
  12. Optogenetic downregulation of protein levels with an ultrasensitive switch. ACS Synth Biol, 2019
  13. Reversible induction of mitophagy by an optogenetic bimodular system. Nat Commun, 2019
  14. Physical Plasma Membrane Perturbation Using Subcellular Optogenetics Drives Integrin-Activated Cell Migration. ACS Synth Biol, 2019
  15. Signaling Dynamics Control Cell Fate in the Early Drosophila Embryo. Dev Cell, 2019
  16. Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds. Cell, 2018
  17. Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome. Cell, 2018
  18. Light-Guided Motility of a Minimal Synthetic Cell. Nano Lett, 2018
  19. Increasing spatial resolution of photoregulated GTPases through immobilized peripheral membrane proteins. Small GTPases, 2018
  20. Reversible Social Self-Sorting of Colloidal Cell-Mimics with Blue Light Switchable Proteins. ACS Synth Biol, 2018
  21. Membrane Flow Drives an Adhesion-Independent Amoeboid Cell Migration Mode. Dev Cell, 2018
  22. Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble. Elife, 2018
  23. Optogenetic reversible knocksideways, laser ablation, and photoactivation on the mitotic spindle in human cells. Methods Cell Biol, 2018
  24. Optogenetic Control of Cell Migration. Methods Mol Biol, 2018
  25. Light-activated protein interaction with high spatial subcellular confinement. Proc Natl Acad Sci USA, 2018
  26. Dynamic blue light-switchable protein patterns on giant unilamellar vesicles. Chem Commun (Camb), 2018
  27. Control of microtubule dynamics using an optogenetic microtubule plus end-F-actin cross-linker. J Cell Biol, 2017
  28. Optogenetic Control of Endoplasmic Reticulum-Mitochondria Tethering. ACS Synth Biol, 2017
  29. Time-gated detection of protein-protein interactions with transcriptional readout. Elife, 2017
  30. Intracellular production of hydrogels and synthetic RNA granules by multivalent molecular interactions. Nat Mater, 2017
  31. Vesicle Docking Is a Key Target of Local PI(4,5)P2 Metabolism in the Secretory Pathway of INS-1 Cells. Cell Rep, 2017
  32. Photocontrolled reversible self-assembly of dodecamer nitrilase. Bioresour Bioprocess, 2017
  33. Cells lay their own tracks: optogenetic Cdc42 activation stimulates fibronectin deposition supporting directed migration. J Cell Sci, 2017
  34. Evolution of a split RNA polymerase as a versatile biosensor platform. Nat Chem Biol, 2017
  35. The Spatiotemporal Limits of Developmental Erk Signaling. Dev Cell, 2017
  36. Tuning the Binding Affinities and Reversion Kinetics of a Light Inducible Dimer Allows Control of Transmembrane Protein Localization. Biochemistry, 2016
  37. Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol, 2016
  38. Subcellular optogenetic activation of Cdc42 controls local and distal signaling to drive immune cell migration. Mol Biol Cell, 2016
  39. Correlating in Vitro and in Vivo Activities of Light-Inducible Dimers: A Cellular Optogenetics Guide. ACS Synth Biol, 2015
  40. Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins. Proc Natl Acad Sci USA, 2014