iLID

Related original publications

1. Dynamic Light-Induced Protein Patterns at Model Membranes. J Vis Exp, 2024
2. Asymmetric oligomerization state and sequence patterning can tune multiphase condensate miscibility. Nat Chem, 2024
3. Interplay of condensation and chromatin binding underlies BRD4 targeting. bioRxiv, 2024
4. Using split protein reassembly strategy to optically control PLD enzymatic activity. bioRxiv, 2024
5. Ultralow Background Membrane Editors for Spatiotemporal Control of Phosphatidic Acid Metabolism and Signaling ACS Cent Sci, 2024
6. Rapid and reversible dissolution of biomolecular condensates using light-controlled recruitment of a solubility tag. bioRxiv, 2024
7. Light-based juxtacrine signaling between synthetic cells. bioRxiv, 2024
8. Photoactivation of LOV domains with chemiluminescence. Chem Sci, 2023
9. A single-component, light-assisted uncaging switch for endoproteolytic release. Nat Chem Biol, 2023
10. Critical capillary waves of biomolecular condensates. bioRxiv, 2023
11. Local negative feedback of Rac activity at the leading edge underlies a pilot pseudopod-like program for amoeboid cell guidance. PLoS Biol, 2023
12. CaaX-motif-adjacent residues influence G protein gamma (Gγ) prenylation under suboptimal conditions. J Biol Chem, 2023
13. Light-activated microtubule-based two-dimensional active nematic. Soft Matter, 2023
14. Control of cell retraction and protrusion with a single protein. bioRxiv, 2023
15. Dynamics of an incoherent feedforward loop drive ERK-dependent pattern formation in the early Drosophila embryo. Development, 2023
16. C-terminal sequence stability profiling in Saccharomyces cerevisiae reveals protective protein quality control pathways. J Biol Chem, 2023
17. Spatiotemporal optical control of Gαq-PLCβ interactions. bioRxiv, 2023
18. Endoplasmic reticulum exit sites are segregated for secretion based on cargo size. bioRxiv, 2023
19. Optogenetic clustering and membrane translocation of the BcLOV4 photoreceptor. Proc Natl Acad Sci U S A, 2023
20. Opto-RhoGEFs, an optimized optogenetic toolbox to reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier strength. Elife, 2023
21. Concept and considerations of a medical device: the active noise cancelling incubator. Front Pediatr, 2023
22. Cell protrusions and contractions generate long-range membrane tension propagation. Cell, 2023
23. Actuation of single downstream nodes in growth factor network steers immune cell migration. Dev Cell, 2023
24. Engineered allostery in light-regulated LOV-Turbo enables precise spatiotemporal control of proximity labeling in living cells. Nat Methods, 2023
25. Self-Regulated and Bidirectional Communication in Synthetic Cell Communities. ACS Nano, 2023
26. Focal adhesions are controlled by microtubules through local contractility regulation. bioRxiv, 2023
27. A disordered tether to iLID improves photoswitchable protein patterning on model membranes. Chem Commun (Camb), 2023
28. Mechanosensitive mTORC2 independently coordinates leading and trailing edge polarity programs during neutrophil migration. Mol Biol Cell, 2023
29. Rac negative feedback links local PIP3 rate-of-change to dynamic control of neutrophil guidance. bioRxiv, 2023
30. Orthogonal Light-Dependent Membrane Adhesion Induces Social Self-Sorting and Member-Specific DNA Communication in Synthetic Cell Communities. Small, 2023
31. Mechanistic insights into cancer drug resistance through optogenetic PI3K signaling hyperactivation. Cell Chem Biol, 2022
32. Integrin-based adhesion compartmentalizes ALK3 of the BMPRII to control cell adhesion and migration. J Cell Biol, 2022
33. Opto-katanin, an optogenetic tool for localized, microtubule disassembly. Curr Biol, 2022
34. Optogenetic control of apical constriction induces synthetic morphogenesis in mammalian tissues. Nat Commun, 2022
35. Defunctionalizing intracellular organelles such as mitochondria and peroxisomes with engineered phospholipase A/acyltransferases. Nat Commun, 2022
36. Visual detection of submicroscopic protein clusters with a phase-separation-based fluorescent reporter. bioRxiv, 2022
37. Dimerization of iLID Optogenetic Proteins Observed Using 3D Single-Molecule Tracking in Live Bacterial Cells. bioRxiv, 2022
38. Synthetic cells with self-activating optogenetic proteins communicate with natural cells. Nat Commun, 2022
39. Motor processivity and speed determine structure and dynamics of microtubule-motor assemblies. bioRxiv, 2022
40. mTORC2 coordinates the leading and trailing edge cytoskeletal programs during neutrophil migration. bioRxiv, 2022
41. Persistent cell migration emerges from a coupling between protrusion dynamics and polarized trafficking. Elife, 2022
42. A rich get richer effect governs intracellular condensate size distributions. bioRxiv, 2022
43. Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells. Front Cell Neurosci, 2022
44. Oncogenic protein condensates modulate cell signal perception and drug tolerance. bioRxiv, 2022
45. Compartmentalization of telomeres through DNA-scaffolded phase separation. Dev Cell, 2022
46. Substratum stiffness regulates Erk signaling dynamics through receptor-level control. Cell Rep, 2021
47. Opto-Katanin: An Optogenetic Tool for Localized Microtubule Disassembly. bioRxiv, 2021
48. Temperature-responsive optogenetic probes of cell signaling. Nat Chem Biol, 2021
49. Gezielte Injektion von Effektoren durch Kontrolle der Proteindynamik. BIOspektrum (Heidelb), 2021
50. An Optogenetic Toolbox for Synergistic Regulation of Protein Abundance. ACS Synth Biol, 2021
51. A synthetic gene circuit for imaging-free detection of signaling pulses. Cell Syst, 2021
52. Nano-optogenetic engineering of CAR T cells for precision immunotherapy with enhanced safety. Nat Nanotechnol, 2021
53. Defunctionalizing Intracellular Organelles with Genetically-Encoded Molecular Tools Based on Engineered Phospholipase A/Acyltransferases (PLAATs). bioRxiv, 2021
54. An active tethering mechanism controls the fate of vesicles. Nat Commun, 2021
55. A Non-Canonical Raf Function Is Required for Dorsal-Ventral Patterning During Drosophila Embryogenesis. bioRxiv, 2021
56. Circularly permuted AsLOV2 as an optogenetic module for engineering photoswitchable peptides. Chem Commun (Camb), 2021
57. Mechanical Frustration of Phase Separation in the Cell Nucleus by Chromatin. Phys Rev Lett, 2021
58. Positive feedback between the T cell kinase Zap70 and its substrate LAT acts as a clustering-dependent signaling switch. Cell Rep, 2021
59. Cell to Cell Signaling through Light in Artificial Cell Communities: Glowing Predator Lures Prey. ACS Nano, 2021
60. mem-iLID, a fast and economic protein purification method. Biosci Rep, 2021
61. Optogenetic model reveals cell shape regulation through FAK and Fascin. J Cell Sci, 2021
62. Temporal integration of inductive cues on the way to gastrulation. Proc Natl Acad Sci U S A, 2021
63. Light-inducible deformation of mitochondria in live cells. Cell Chem Biol, 2021
64. Engineering a Blue Light Inducible SpyTag System (BLISS). J Am Chem Soc, 2021
65. Bioluminescent Synthetic Cells Communicate with Natural Cells and Self-Activate Light-Responsive Proteins. bioRxiv, 2021
66. Circularly permuted LOV2 as a modular photoswitch for optogenetic engineering. Nat Chem Biol, 2021
67. Endothelial cell invasion is controlled by dactylopodia. Proc Natl Acad Sci U S A, 2021
68. Dynamics and heterogeneity of Erk-induced immediate-early gene expression. bioRxiv, 2021
69. Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis. bioRxiv, 2021
70. Optimized iLID Membrane Anchors for Local Optogenetic Protein Recruitment. ACS Synth Biol, 2021
71. Coupling between protrusion dynamics and polarized trafficking steers persistent cell migration. bioRxiv, 2021
72. T cells selectively filter oscillatory signals on the minutes timescale. Proc Natl Acad Sci U S A, 2021
73. Optogenetic control of PRC1 reveals its role in chromosome alignment on the spindle by overlap length-dependent forces. Elife, 2021
74. Optogenetic control of small GTPases reveals RhoA mediates intracellular calcium signaling. J Biol Chem, 2021
75. Optogenetic inhibition and activation of Rac and Rap1 using a modified iLID system. bioRxiv, 2020
76. Rho1 activation recapitulates early gastrulation events in the ventral, but not dorsal, epithelium of Drosophila embryos. Elife, 2020
77. Light-inducible Deformation of Mitochondria in Live Cells. bioRxiv, 2020
78. Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold. Nat Commun, 2020
79. Optogenetic control of protein binding using light-switchable nanobodies. Nat Commun, 2020
80. Engineering combinatorial and dynamic decoders using synthetic immediate-early genes. Commun Biol, 2020
81. Multistimuli Sensing Adhesion Unit for the Self-Positioning of Minimal Synthetic Cells. Small, 2020
82. Dynamic centriolar localization of Polo and Centrobin in early mitosis primes centrosome asymmetry. PLoS Biol, 2020
83. Heterogeneous somatostatin-expressing neuron population in mouse ventral tegmental area. Elife, 2020
84. Photo-SNAP-tag, a Light-Regulated Chemical Labeling System. ACS Chem Biol, 2020
85. LITESEC-T3SS - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution. Nat Commun, 2020
86. Photoactivatable Cre recombinase 3.0 for in vivo mouse applications. Nat Commun, 2020
87. Construction of Light-Activated Neurotrophin Receptors Using the Improved Light-Induced Dimerizer (iLID). J Mol Biol, 2020
88. An optimized toolbox for the optogenetic control of intracellular transport. J Cell Biol, 2020
89. Implementing Optogenetic Modulation in Mechanotransduction. Phys Rev X, 2020
90. ActuAtor, a molecular tool for generating force in living cells: Controlled deformation of intracellular structures. bioRxiv, 2020
91. Light-inducible generation of membrane curvature in live cells with engineered BAR domain proteins. ACS Synth Biol, 2020
92. A Live-Cell Screen for Altered Erk Dynamics Reveals Principles of Proliferative Control. Cell Syst, 2020
93. SRRF-stream imaging of optogenetically controlled furrow formation shows localized and coordinated endocytosis and exocytosis mediating membrane remodeling. ACS Synth Biol, 2020
94. Rapid Dynamics of Signal-Dependent Transcriptional Repression by Capicua. Dev Cell, 2020
95. Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling. Nat Commun, 2020
96. Tunable light and drug induced depletion of target proteins. Nat Commun, 2020
97. Dynamic organelle distribution initiates actin-based spindle migration in mouse oocytes. Nat Commun, 2020
98. Ras acts as a molecular switch between two forms of consolidated memory in Drosophila. Proc Natl Acad Sci USA, 2020
99. Engineered BRET-Based Biologic Light Sources Enable Spatiotemporal Control over Diverse Optogenetic Systems. ACS Synth Biol, 2019
100. Optimizing photoswitchable MEK. Proc Natl Acad Sci USA, 2019
101. The importance of cell-cell interaction dynamics in bottom-up tissue engineering: Concepts of colloidal self-assembly in the fabrication of multicellular architectures. Nano Lett, 2019
102. Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins. Nat Methods, 2019
103. Light controlled cell-to-cell adhesion and chemical communication in minimal synthetic cells. Chem Commun (Camb), 2019
104. High-throughput multicolor optogenetics in microwell plates. Nat Protoc, 2019
105. Optogenetic downregulation of protein levels with an ultrasensitive switch. ACS Synth Biol, 2019
106. Reversible induction of mitophagy by an optogenetic bimodular system. Nat Commun, 2019
107. Physical Plasma Membrane Perturbation Using Subcellular Optogenetics Drives Integrin-Activated Cell Migration. ACS Synth Biol, 2019
108. Signaling Dynamics Control Cell Fate in the Early Drosophila Embryo. Dev Cell, 2019
109. A Photoactivatable Botulinum Neurotoxin for Inducible Control of Neurotransmission. Neuron, 2019
110. Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds. Cell, 2018
111. Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome. Cell, 2018
112. Light-Guided Motility of a Minimal Synthetic Cell. Nano Lett, 2018
113. Increasing spatial resolution of photoregulated GTPases through immobilized peripheral membrane proteins. Small GTPases, 2018
114. Reversible Social Self-Sorting of Colloidal Cell-Mimics with Blue Light Switchable Proteins. ACS Synth Biol, 2018
115. Membrane Flow Drives an Adhesion-Independent Amoeboid Cell Migration Mode. Dev Cell, 2018
116. Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble. Elife, 2018
117. Optogenetic reversible knocksideways, laser ablation, and photoactivation on the mitotic spindle in human cells. Methods Cell Biol, 2018
118. Optogenetic Control of Cell Migration. Methods Mol Biol, 2018
119. Light-activated protein interaction with high spatial subcellular confinement. Proc Natl Acad Sci USA, 2018
120. Dynamic blue light-switchable protein patterns on giant unilamellar vesicles. Chem Commun (Camb), 2018
121. Control of microtubule dynamics using an optogenetic microtubule plus end-F-actin cross-linker. J Cell Biol, 2017
122. Optogenetic Control of Endoplasmic Reticulum-Mitochondria Tethering. ACS Synth Biol, 2017
123. Time-gated detection of protein-protein interactions with transcriptional readout. Elife, 2017
124. Intracellular production of hydrogels and synthetic RNA granules by multivalent molecular interactions. Nat Mater, 2017
125. Vesicle Docking Is a Key Target of Local PI(4,5)P2 Metabolism in the Secretory Pathway of INS-1 Cells. Cell Rep, 2017
126. Photocontrolled reversible self-assembly of dodecamer nitrilase. Bioresour Bioprocess, 2017
127. Cells lay their own tracks: optogenetic Cdc42 activation stimulates fibronectin deposition supporting directed migration. J Cell Sci, 2017
128. Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain. Nat Methods, 2017
129. Evolution of a split RNA polymerase as a versatile biosensor platform. Nat Chem Biol, 2017
130. The Spatiotemporal Limits of Developmental Erk Signaling. Dev Cell, 2017
131. Tuning the Binding Affinities and Reversion Kinetics of a Light Inducible Dimer Allows Control of Transmembrane Protein Localization. Biochemistry, 2016
132. Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol, 2016
133. Subcellular optogenetic activation of Cdc42 controls local and distal signaling to drive immune cell migration. Mol Biol Cell, 2016
134. Correlating in Vitro and in Vivo Activities of Light-Inducible Dimers: A Cellular Optogenetics Guide. ACS Synth Biol, 2015
135. Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins. Proc Natl Acad Sci USA, 2014
136. Optogenetic actuator - ERK biosensor circuits identify MAPK network nodes that shape ERK dynamics. Mol Syst Biol, 2022
137. Using Optogenetics to Spatially Control Cortical Dynein Activity in Mitotic Human Cells. Methods Mol Biol, 2023
138. Application of Optogenetics to Probe the Signaling Dynamics of Cell Fate Decision-Making. Methods Mol Biol, 2023
139. Nano-optogenetic CAR-T Cell Immunotherapy. Methods Mol Biol, 2024