iLID

Photoreceptor AsLOV2–SsrA
Binding partner SspB
Cofactor FMN
Source organism Avena sativa
Mode of action heterodimerization
Excitation wavelength 450 nm
Reversion wavelength dark
Excitation time seconds
Reversion time seconds to minutes (variants available)
Crystal Structure of the iLID heterodimer. (PDB ID: 4WF0)

Related original publications

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  1. An Optogenetic Toolbox for Synergistic Regulation of Protein Abundance. ACS Synth Biol, 2021
  2. Defunctionalizing Intracellular Organelles with Genetically-Encoded Molecular Tools Based on Engineered Phospholipase A/Acyltransferases (PLAATs). bioRxiv, 2021
  3. An active tethering mechanism controls the fate of vesicles. Nat Commun, 2021
  4. A Non-Canonical Raf Function Is Required for Dorsal-Ventral Patterning During Drosophila Embryogenesis. bioRxiv, 2021
  5. Optogenetic actuator/biosensor circuits for large-scale interrogation of ERK dynamics identify sources of MAPK signaling robustness. bioRxiv, 2021
  6. Circularly permuted AsLOV2 as an optogenetic module for engineering photoswitchable peptides. Chem Commun (Camb), 2021
  7. Mechanical Frustration of Phase Separation in the Cell Nucleus by Chromatin. Phys Rev Lett, 2021
  8. Positive feedback between the T cell kinase Zap70 and its substrate LAT acts as a clustering-dependent signaling switch. Cell Rep, 2021
  9. mem-iLID, a fast and economic protein purification method. Biosci Rep, 2021
  10. Optogenetic model reveals cell shape regulation through FAK and Fascin. J Cell Sci, 2021
  11. Temporal integration of inductive cues on the way to gastrulation. Proc Natl Acad Sci U S A, 2021
  12. Light-inducible deformation of mitochondria in live cells. Cell Chem Biol, 2021
  13. Engineering a Blue Light Inducible SpyTag System (BLISS). J Am Chem Soc, 2021
  14. Bioluminescent Synthetic Cells Communicate with Natural Cells and Self-Activate Light-Responsive Proteins. bioRxiv, 2021
  15. Circularly permuted LOV2 as a modular photoswitch for optogenetic engineering. Nat Chem Biol, 2021
  16. Dynamics and heterogeneity of Erk-induced immediate-early gene expression. bioRxiv, 2021
  17. Optogenetic control of apical constriction induces synthetic morphogenesis in mammalian tissues. bioRxiv, 2021
  18. Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis. bioRxiv, 2021
  19. Optimized iLID Membrane Anchors for Local Optogenetic Protein Recruitment. ACS Synth Biol, 2021
  20. Coupling between protrusion dynamics and polarized trafficking steers persistent cell migration. bioRxiv, 2021
  21. T cells selectively filter oscillatory signals on the minutes timescale. Proc Natl Acad Sci U S A, 2021
  22. Optogenetic control of PRC1 reveals its role in chromosome alignment on the spindle by overlap length-dependent forces. Elife, 2021
  23. Optogenetic control of small GTPases reveals RhoA mediates intracellular calcium signaling. J Biol Chem, 2021
  24. A synthetic gene circuit for imaging-free detection of dynamic cell signaling. bioRxiv, 2021
  25. Mechanical frustration of phase separation in the cell nucleus by chromatin. bioRxiv, 2020
  26. Optogenetic inhibition and activation of Rac and Rap1 using a modified iLID system. bioRxiv, 2020
  27. Rho1 activation recapitulates early gastrulation events in the ventral, but not dorsal, epithelium of Drosophila embryos. Elife, 2020
  28. Light-inducible Deformation of Mitochondria in Live Cells. bioRxiv, 2020
  29. Clustering-based positive feedback between a kinase and its substrate enables effective T-cell receptor signaling. bioRxiv, 2020
  30. Endothelial cell invasiveness is controlled by myosin IIA-dependent inhibition of Arp2/3 activity. bioRxiv, 2020
  31. Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold. Nat Commun, 2020
  32. Optogenetic control of protein binding using light-switchable nanobodies. Nat Commun, 2020
  33. Engineering combinatorial and dynamic decoders using synthetic immediate-early genes. Commun Biol, 2020
  34. Dynamic centriolar localization of Polo and Centrobin in early mitosis primes centrosome asymmetry. PLoS Biol, 2020
  35. Heterogeneous somatostatin-expressing neuron population in mouse ventral tegmental area. Elife, 2020
  36. Photo-SNAP-tag, a Light-Regulated Chemical Labeling System. ACS Chem Biol, 2020
  37. LITESEC-T3SS - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution. Nat Commun, 2020
  38. Photoactivatable Cre recombinase 3.0 for in vivo mouse applications. Nat Commun, 2020
  39. Construction of Light-Activated Neurotrophin Receptors Using the Improved Light-Induced Dimerizer (iLID). J Mol Biol, 2020
  40. An optimized toolbox for the optogenetic control of intracellular transport. J Cell Biol, 2020
  41. Implementing Optogenetic Modulation in Mechanotransduction. Phys Rev X, 2020
  42. ActuAtor, a molecular tool for generating force in living cells: Controlled deformation of intracellular structures. bioRxiv, 2020
  43. Light-inducible generation of membrane curvature in live cells with engineered BAR domain proteins. ACS Synth Biol, 2020
  44. A Live-Cell Screen for Altered Erk Dynamics Reveals Principles of Proliferative Control. Cell Syst, 2020
  45. SRRF-stream imaging of optogenetically controlled furrow formation shows localized and coordinated endocytosis and exocytosis mediating membrane remodeling. ACS Synth Biol, 2020
  46. Rapid Dynamics of Signal-Dependent Transcriptional Repression by Capicua. Dev Cell, 2020
  47. Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling. Nat Commun, 2020
  48. Tunable light and drug induced depletion of target proteins. Nat Commun, 2020
  49. Dynamic organelle distribution initiates actin-based spindle migration in mouse oocytes. Nat Commun, 2020
  50. Ras acts as a molecular switch between two forms of consolidated memory in Drosophila. Proc Natl Acad Sci USA, 2020
  51. Engineered BRET-Based Biologic Light Sources Enable Spatiotemporal Control over Diverse Optogenetic Systems. ACS Synth Biol, 2019
  52. Optimizing photoswitchable MEK. Proc Natl Acad Sci USA, 2019
  53. The importance of cell-cell interaction dynamics in bottom-up tissue engineering: Concepts of colloidal self-assembly in the fabrication of multicellular architectures. Nano Lett, 2019
  54. Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins. Nat Methods, 2019
  55. Light controlled cell-to-cell adhesion and chemical communication in minimal synthetic cells. Chem Commun (Camb), 2019
  56. High-throughput multicolor optogenetics in microwell plates. Nat Protoc, 2019
  57. Optogenetic downregulation of protein levels with an ultrasensitive switch. ACS Synth Biol, 2019
  58. Reversible induction of mitophagy by an optogenetic bimodular system. Nat Commun, 2019
  59. Physical Plasma Membrane Perturbation Using Subcellular Optogenetics Drives Integrin-Activated Cell Migration. ACS Synth Biol, 2019
  60. Signaling Dynamics Control Cell Fate in the Early Drosophila Embryo. Dev Cell, 2019
  61. Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds. Cell, 2018
  62. Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome. Cell, 2018
  63. Light-Guided Motility of a Minimal Synthetic Cell. Nano Lett, 2018
  64. Increasing spatial resolution of photoregulated GTPases through immobilized peripheral membrane proteins. Small GTPases, 2018
  65. Reversible Social Self-Sorting of Colloidal Cell-Mimics with Blue Light Switchable Proteins. ACS Synth Biol, 2018
  66. Membrane Flow Drives an Adhesion-Independent Amoeboid Cell Migration Mode. Dev Cell, 2018
  67. Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble. Elife, 2018
  68. Optogenetic reversible knocksideways, laser ablation, and photoactivation on the mitotic spindle in human cells. Methods Cell Biol, 2018
  69. Optogenetic Control of Cell Migration. Methods Mol Biol, 2018
  70. Light-activated protein interaction with high spatial subcellular confinement. Proc Natl Acad Sci USA, 2018
  71. Dynamic blue light-switchable protein patterns on giant unilamellar vesicles. Chem Commun (Camb), 2018
  72. Control of microtubule dynamics using an optogenetic microtubule plus end-F-actin cross-linker. J Cell Biol, 2017
  73. Optogenetic Control of Endoplasmic Reticulum-Mitochondria Tethering. ACS Synth Biol, 2017
  74. Time-gated detection of protein-protein interactions with transcriptional readout. Elife, 2017
  75. Intracellular production of hydrogels and synthetic RNA granules by multivalent molecular interactions. Nat Mater, 2017
  76. Vesicle Docking Is a Key Target of Local PI(4,5)P2 Metabolism in the Secretory Pathway of INS-1 Cells. Cell Rep, 2017
  77. Photocontrolled reversible self-assembly of dodecamer nitrilase. Bioresour Bioprocess, 2017
  78. Cells lay their own tracks: optogenetic Cdc42 activation stimulates fibronectin deposition supporting directed migration. J Cell Sci, 2017
  79. Evolution of a split RNA polymerase as a versatile biosensor platform. Nat Chem Biol, 2017
  80. The Spatiotemporal Limits of Developmental Erk Signaling. Dev Cell, 2017
  81. Tuning the Binding Affinities and Reversion Kinetics of a Light Inducible Dimer Allows Control of Transmembrane Protein Localization. Biochemistry, 2016
  82. Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol, 2016
  83. Subcellular optogenetic activation of Cdc42 controls local and distal signaling to drive immune cell migration. Mol Biol Cell, 2016
  84. Correlating in Vitro and in Vivo Activities of Light-Inducible Dimers: A Cellular Optogenetics Guide. ACS Synth Biol, 2015
  85. Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins. Proc Natl Acad Sci USA, 2014